scholarly journals Functional and molecular characterization of the transcriptional regulatory region of Tcp-10bt, a testes-expressed gene from the t complex responder locus

Development ◽  
1993 ◽  
Vol 117 (1) ◽  
pp. 89-95 ◽  
Author(s):  
U.K. Ewulonu ◽  
T.J. Buratynski ◽  
J.C. Schimenti
Keyword(s):  
Germ Cell ◽  
Molecular Characterization ◽  
Regulatory Region ◽  
Transmission Ratio Distortion ◽  
Cell Protein ◽  
Specific Expression ◽  
T Complex ◽  
Transcriptional Regulatory ◽  
Ratio Distortion

Mouse t haplotypes contain several mutant alleles that disrupt spermatogenesis. Their phenotypes include sterility, reduced fertility and transmission ratio distortion (TRD). The substantial genetic analyses of these mutant alleles, coupled with intensive physical characterization of the t complex, provides a fertile ground for identifying and understanding genes essential to male gametogenesis. The t complex responder (Tcr) locus plays a central role in this process, interacting with other t haplotype-encoded genes to mediate TRD. A candidate responder gene, Tcp-10bt, has been cloned and subjected to molecular characterization. Here, we define the transcriptional regulatory regions of this gene in transgenic mice. A 1.6 kb (but not 0.6 kb) DNA fragment upstream of the transcription start site contains all the regulatory signals for appropriate temporal and germ cell-specific expression of this gene. Two smaller fragments within this region bound specifically to nuclear factor(s) from germ cell protein extracts in gel shift assays. This work is a step towards understanding the mechanism of Tcp-10bt regulated expression and may ultimately help reveal a common regulatory pathway shared by other similarly expressed spermatogenic genes.

scholarly journals A structural gene (Tcp-1) within the mouse t complex is separable from effects on tail length and lethality but may be associated with effects on spermatogenesis

1981 ◽  
Vol 38 (2) ◽  
pp. 115-123 ◽  
Author(s):  
Lee M. Silver
Keyword(s):  
Genetic Basis ◽  
Genetic Factors ◽  
Tail Length ◽  
Transmission Ratio Distortion ◽  
Transmission Ratio ◽  
Cell Protein ◽  
Wild Type ◽  
T Complex ◽  
Interaction Factor ◽  
Ratio Distortion

SUMMARYThe Tcp-1 gene is located within the t complex and codes for a major testicular cell protein called p63/6.9. All wild-type chromosomes carry the Tcp-1b allele which codes for a basic form of this protein, while all complete t haplotypes carry the Tcp-1a allele which codes for an acidic form of this protein. It is not clear whether the Tcp-1 gene is associated with phenotypic effects of t haplotypes on embryogenesis and/or spermatogenesis, since the genetic basis for these effects is extremely complex. The elegant analysis of Lyon & Mason (1977) has allowed the identification and separation of a family of genetic factors which interact to produce the observed phenotypes associated with various combinations of t haplotypes. The data summarized in this report indicate that the Tcp-1a locus is separable from all of the identified t haplotype factors except for one; a complete correlation has been obtained between Tcp-1a and a proximal t haplotype factor which is involved in effects on transmission ratio distortion. Two other novel points emerge from this analysis. First, it appears that the tail interaction factor and the proximal sperm factors represent distinct genetic loci. Second, the accumulated data lead to the proposal that the TOrl chromosome carries a short segment of t haplotype chromatin containing Tcp-1a and proximal sperm factors involved in transmission ratio distortion and sterility.

scholarly journals Gene dosage effects on transmission ratio distortion and fertility in mice that carry t haplotypes

1989 ◽  
Vol 54 (3) ◽  
pp. 221-225 ◽  
Author(s):  
Lee M. Silver
Keyword(s):  
Gene Dosage ◽  
Transmission Ratio Distortion ◽  
Transmission Ratio ◽  
Double Dose ◽  
Absolute Level ◽  
T Complex ◽  
Gene Dosage Effects ◽  
Ratio Distortion ◽  
The Individual ◽  
Very High

SummaryComplete t haplotypes can be transmitted at distorted ratios from heterozygous +/t male mice as a consequence of t-specific alleles at a series of t complex distorter loci (Tcd-1t through Tcd-4t) and a t complex responder locus. Partial t haplotypes that lack the Tcd-2t allele cannot be transmitted at the very high ratios characteristic of complete t haplotypes. The breeding studies reported here tested the possibility that the absence of Tcd-2t could be compensated for by the presence of double doses of other Tcdt alleles. The results indicate that a double dose of Tcd-4t alone will not work, but that a double dose of both Tcd-1t and Tcd-4t can promote a very high transmission ratio in the absence of Tcd-2t. These results suggest that the extent to which transmission ratios are distorted is dependent upon the absolute level of expression of the individual Tcd genes. Further studies of genotypic effects on transmission ratio distortion, as well as fertility, lead to the suggestion of a fifth t complex distorter (Tcd-5) locus within t haplotypes.

Molecular Characterization of the hnRNP A2/B1 Proteins: Tissue-Specific Expression and Novel Isoforms

1999 ◽  
Vol 246 (2) ◽  
pp. 399-411 ◽  
Author(s):  
Hiroshi Kamma ◽  
Hisashi Horiguchi ◽  
Lili Wan ◽  
Miwa Matsui ◽  
Masachika Fujiwara ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Novel Isoforms

Germ Cell-Specific Expression and Biochemical Characterization of Mouse PAD6.

2008 ◽  
Vol 78 (Suppl_1) ◽  
pp. 297-297
Author(s):  
Keiichiro Yogo ◽  
Grisnarong Wongbandue ◽  
Akihito Ishigami ◽  
Hidenari Takahara ◽  
Tetsuya Kohsaka
Keyword(s):  
Germ Cell ◽  
Biochemical Characterization ◽  
Specific Expression

Characterization of the 5′-flanking transcriptional regulatory region of the human Fcγ receptor gene, Fcγ RIIA

1992 ◽  
Vol 29 (10) ◽  
pp. 1165-1174 ◽  
Author(s):  
Steven E. McKenzie ◽  
Margaret A. Keller ◽  
Diana L. Cassel ◽  
Alan D. Schreiber ◽  
Elias Schwartz ◽  
...  
Keyword(s):  
Receptor Gene ◽  
Regulatory Region ◽  
Fcγ Receptor ◽  
Transcriptional Regulatory ◽  
Transcriptional Regulatory Region
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