Orthogonal Polarization Spectral Imaging Versus Intravital Fluorescent Microscopy for Microvascular Studies in Wounds

2002 ◽  
Vol 48 (6) ◽  
pp. 646-653 ◽  
Author(s):  
S. Langer ◽  
F. Born ◽  
R. Hatz ◽  
P. Biberthaler ◽  
K. Messmer
Keyword(s):  
Fluorescent Microscopy ◽  
Spectral Imaging ◽  
Orthogonal Polarization ◽  
Orthogonal Polarization Spectral ◽  
Intravital Fluorescent Microscopy

Intraoperative Detection of Early Microvasospasm in Patients with Subarachnoid Hemorrhage by Using Orthogonal Polarization Spectral Imaging

Neurosurgery ◽  
2003 ◽  
Vol 52 (6) ◽  
pp. 1307-1317 ◽  
Author(s):  
Eberhard Uhl ◽  
Jens Lehmberg ◽  
Hans-Jakob Steiger ◽  
Konrad Messmer
Keyword(s):  
Subarachnoid Hemorrhage ◽  
Quantitative Analysis ◽  
Spectral Imaging ◽  
Capillary Density ◽  
Orthogonal Polarization ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative ◽  
Cell Velocity ◽  
Orthogonal Polarization Spectral ◽  
Red Blood Cell Velocity

Abstract OBJECTIVE Changes of major cerebral vessels in patients with subarachnoid hemorrhage (SAH) are well known from routine cerebral angiography. Data on changes in the microcirculation do not exist. This study sought to provide a qualitative and quantitative analysis of the cortical microcirculation after SAH. METHODS By means of orthogonal polarization spectral imaging, a qualitative and quantitative analysis of cortical microcirculation was performed during aneurysm surgery in 3 patients with an incidental intracerebral aneurysm and 10 patients with SAH. Vessel diameters, red blood cell velocity, and functional capillary density were analyzed before and after the aneurysm was clipped. RESULTS Initial capillary density in patients with an incidental aneurysm was 91.5 ± 36.5 cm−1 (mean ± standard deviation) compared with 30.5 ± 13.8 in patients with SAH (P < 0.05). In patients with SAH, capillary density increased significantly to 53.9 ± 29.1 cm−1 (P < 0.05) during the operation, as did the frequency of venules with a red blood cell velocity greater than 2 mm/s (P < 0.05). No significant change of arteriolar or venular diameters was observed. However, in patients with SAH, mono- and multisegmental microvasospasms in arterioles were observed, with a reduction of vessel diameters up to 75.1%. CONCLUSION Orthogonal polarization spectral imaging is a suitable method to study cerebral microcirculation during surgery. In patients with SAH, capillary density is significantly decreased and small arteries and arterioles of the cortical surface exhibit vasospasm that cannot be detected by angiography or transcranial Doppler sonography. These changes may contribute to the initial clinical symptoms and may have an influence on the clinical postoperative course.

scholarly journals Noninvasive Orthogonal Polarization Spectral Imaging as Applied to Microvascular Studies in Mice

2004 ◽  
Vol 5 (3) ◽  
pp. 211-217 ◽  
Author(s):  
P. Nivoit ◽  
A. M. Chevrier ◽  
M. Lagarde ◽  
C. Renaudin ◽  
N. Wiernsperger
Keyword(s):  
Spectral Imaging ◽  
Diabetic Microangiopathy ◽  
Orthogonal Polarization ◽  
High Contrast ◽  
Hormonal Stimulation ◽  
Orthogonal Polarization Spectral ◽  
Before And After ◽  
Topical Applications

In vivo observations of the mouse microcirculation can hardly be performed due to technical difficulties, limiting the knowledge that could be obtained from gene manipulated mice models. The aim of the present study was to check the applicability of a novel optical system, the orthogonal polarization spectral technology, to study the mouse microcirculation. In anaesthetized mice, the spinotrapezius muscle microcirculation was observed in situ. The diameter of precapillary arterioles was measured before and after a pharmacological or hormonal stimulation. High-contrast images of the muscle microcirculation were obtained and significant vasodilatation of arterioles was observed after topical applications of acetylcholine, sodium nitroprusside, and insulin. As compared to conventional techniques, orthogonal polarization spectral imaging makes it possible to assess and study microvascular beds in mice, which were inaccessible until now, allowing the use of gene manipulated mice to investigate, for example, the mechanisms involved in the development of diabetic microangiopathy.

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