Effects of voluntary ethanol intake on the expression of Ca2+/calmodulin-dependent protein kinase IV and on CREB expression and phosphorylation in the rat nucleus accumbens

Neuroreport ◽  
2001 ◽  
Vol 12 (18) ◽  
pp. 4133-4137 ◽  
Author(s):  
Kaushik Misra ◽  
Adip Roy ◽  
Subhash C. Pandey
Keyword(s):  
Nucleus Accumbens ◽  
Protein Kinase ◽  
Ethanol Intake ◽  
Dependent Protein Kinase ◽  
Dependent Protein ◽  
Rat Nucleus Accumbens

8-pCPT, an Epac activator, impairs conditioned place preference based on nucleus accumbens amphetamine in rats

2013 ◽  
Vol 26 (2) ◽  
pp. 104-111 ◽  
Author(s):  
Sung Woo Park ◽  
Ali Roohbakhsh ◽  
Richard J. Beninger
Keyword(s):  
Nucleus Accumbens ◽  
Protein Kinase ◽  
Dopamine Receptor ◽  
Conditioned Place Preference ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Place Preference ◽  
Dependent Protein Kinase ◽  
Cyclic Monophosphate ◽  
Dependent Protein

ObjectivesDopamine receptor-mediated 3′,5′-cyclic adenosine monophosphate (cAMP)-dependent intracellular signalling is important for reward-related learning. cAMP activates cAMP-dependent protein kinase (PKA) and exchange protein directly activated by cAMP (Epac). We tested the hypothesis that reward-related learning may be mediated by Epac.MethodsWe evaluated conditioned place preference (CPP) on the basis of nucleus accumbens (NAc) injections of amphetamine (20 μg/0.5 μl/side) plus Sp-adenosine 3′,5′-cyclic monophosphorothioate triethylamanine (Sp-cAMPS) (0.1, 1.0, 10, 15, 20 μg/0.5 μl/side), an activator of both PKA and Epac, or amphetamine (20 μg) plus 8-(4-chlorophenylthio)-2′-O-methyladenosine-3′,5′-cyclic monophosphate (8-pCPT) (0.73, 1.27, 1.45, 2.89, 5.78, 11.56 μg/0.5 μl/side), an activator of Epac.ResultsIn agreement with previous results, Sp-cAMPS dose-dependently impaired CPP. 8-pCPT impaired CPP at one dose (1.45 μg/0.5 μl/side) and we replicated this effect three times.ConclusionThe results implicate Epac in the acquisition of reward-related learning.

Sign in / Sign up

Export Citation Format

Share Document