Plasma Lecithin Cholesterol Acyltransferase Activity in Severely Burned Patients

1982 ◽  
Vol 3 (3) ◽  
pp. 150-153
Author(s):  
Jun Sasaki ◽  
G. L. Cottam ◽  
Charles R. Baxter
Keyword(s):  
Cholesterol Acyltransferase ◽  
Acyltransferase Activity ◽  
Lecithin Cholesterol Acyltransferase

Effect of Ajuga iva on enzymes involved in the metabolism of cholesterol, in rat fed a cholesterol-enriched diet

2019 ◽  
Vol 50 (2) ◽  
pp. 303-313
Author(s):  
Sherazede Bouderbala ◽  
Malika Bouchenak
Keyword(s):  
Design Methodology ◽  
Cholesterol Metabolism ◽  
Cholesterol Acyltransferase ◽  
Cholesteryl Esters ◽  
Unesterified Cholesterol ◽  
Acyltransferase Activity ◽  
Content Type ◽  
Lecithin Cholesterol Acyltransferase ◽  
Male Wistar Rats ◽  
Adaptation Phase

Purpose This study aims to investigate the effect of Ajuga iva (Ai) on enzymes involved in the metabolism of cholesterol, in rat fed a cholesterol-enriched diet. Design/methodology/approach Male Wistar rats (n = 12), weighing 120 ± 5 g were fed on 1 per cent cholesterol-enriched diet [hypercholesterolemic (HC)] for 15 days (d15). After this adaptation phase, HC rats (total cholesterol = 6.5 ± 0.6 mmol/L) were divided into two groups fed the same diet and treated (Ai-HC) or not with (HC) with Ai for d15. Findings At day 15, in Ai-HC group compared to HC, serum triacylglycerol (TG) values were 1.4-fold lower (p = 0.002), whereas unesterified cholesterol (UC) contents were 1.8-fold higher (p = 0.0001). Serum phospholipids (PL) and cholesteryl esters (CE) contents and liver TG, UC, PL and CE values were not sensitive to Ai. TC/HDL-C and LDL-HDL1-C/HDL-C ratios were, respectively, 1.8- and 4-fold lower (p = 0.006 and p = 0.04). HDL2-C and HDL3-C amounts were enhanced by 40 and 74 per cent, respectively (p = 0.003 and p = 0.0001). HDL3-UC was 1.6-fold higher (p = 0.006); whereas PL contents were 1.4-fold lower (p = 0.003). HDL3-apo and HDL2-CE contents were similar between groups. A decreased of hydroxy-methyl-glutaryl-coenzyme A reductase and cholesterol 7α-hydroxylase activities (−44 and −25 per cent; p = 0.003 and p = 0.02, respectively) were noted. Lecithin: cholesterol acyltransferase activity was 1.5-fold higher (p = 0.001). Originality/value In HC rat, Ai is able to induce hypotriglyceridemia. However, it turns out that Ai may reduce cardiovascular risk by decreasing the reports of atherogenicity and modifying the activities of enzymes involved in the cholesterol metabolism.

The association between lecithin–cholesterol acyltransferase activity and fatty liver index

2019 ◽  
Vol 56 (5) ◽  
pp. 583-592 ◽  
Author(s):  
Jelena Janac ◽  
Aleksandra Zeljkovic ◽  
Zorana Jelic-Ivanovic ◽  
Vesna Dimitrijevic-Sreckovic ◽  
Milica Miljkovic ◽  
...  
Keyword(s):  
High Density Lipoprotein ◽  
Fatty Liver ◽  
Cholesterol Acyltransferase ◽  
Density Lipoprotein ◽  
High Density ◽  
Paraoxonase 1 ◽  
Acyltransferase Activity ◽  
Lecithin Cholesterol Acyltransferase ◽  
Liver Index ◽  
Fatty Liver Index

Background Non-alcoholic fatty liver disease is a frequent ailment with known complications, including those within the cardiovascular system. Associations between several indicators of high-density lipoprotein metabolism and function with clinical and laboratory parameters for the assessment of fatty liver index, a surrogate marker of non-alcoholic fatty liver disease, were evaluated. Methods The study comprised 130 patients classified according to fatty liver index values: fatty liver index < 30, fatty liver index 30–59 (the intermediate group) and fatty liver index ⩾ 60. Lecithin–cholesterol acyltransferase and cholesteryl ester transfer protein activities were determined. Paraoxonase 1 concentration and its activity, paraoxonase 3 concentration and high-density lipoprotein subclass distribution were assessed. Results Increased lecithin–cholesterol acyltransferase activity correlated with increased fatty liver index ( P < 0.001). Paraoxonase 3 concentration was lower in the fatty liver index ⩾ 60 group compared with the fatty liver index < 30 group ( P < 0.05). Cholesteryl ester transfer protein activity, paraoxonase 1 concentration and its activity did not significantly differ across the fatty liver index groups. The relative proportion of small-sized high-density lipoprotein 3 subclass was higher in the fatty liver index ⩾ 60 group compared with the other two fatty liver index groups ( P < 0.01). Lecithin–cholesterol acyltransferase activity positively associated with the fatty liver index ⩾ 60 group and remained significant after adjustment for other potential confounders. Only the triglyceride concentration remained significantly associated with lecithin–cholesterol acyltransferase activity when the parameters that constitute the fatty liver index equation were examined. Conclusions Higher lecithin–cholesterol acyltransferase activity is associated with elevated fatty liver index values. Significant independent association between triglycerides and lecithin–cholesterol acyltransferase activity might indicate a role of hypertriglyceridaemia in alterations of lecithin–cholesterol acyltransferase activity in individuals with elevated fatty liver index.

Investigation of Serum Lipoproteins and Apoproteins in Abetalipoproteinaemia

1974 ◽  
Vol 46 (4) ◽  
pp. 457-468
Author(s):  
G. Kostner ◽  
A. Holasek ◽  
H. G. Bohlmann ◽  
H. Thiede
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Cholesterol Acyltransferase ◽  
Normal Subjects ◽  
Normal Serum ◽  
High Density ◽  
High Density Lipoproteins ◽  
Total Serum ◽  
Serum Lipoproteins ◽  
Acyltransferase Activity ◽  
Lecithin Cholesterol Acyltransferase

1. The serum lipoproteins, apoproteins and lecithin—cholesterol acyltransferase activity of three patients with abetalipoproteinaemia have been studied. 2. Concentration of lipoproteins with a density of less than 1·063 was found to be 5–6 mg/100 ml of plasma. The protein part consisted only of apoAI polypeptides. 3. Concentration of total serum high-density lipoproteins was 90–100 mg/100 ml. After total delipidization of this fraction, apoproteins were separated by column chromatography. All apoA and most of the apoC peptides could be demonstrated by immunochemical methods and polyacrylamide gel electrophoresis. 4. Quantification of individual polypeptides indicated that the proportions apoAI: apoAII: apoAIII in high-density lipoproteins were not different from those observed in normal subjects. ApoCIII1 was absent in the patients' serum. In the remaining apoC peptides differences in the distribution compared with normal subjects could be demonstrated. 5. With antibodies to lipoprotein B, acetylated lipoprotein B or apolipoprotein B no reaction could be observed with abetalipoproteinaemic serum or any density fraction tested including density 1·23 infranate. 6. Isoelectric focussing of prestained lipoproteins in complete patients' serum revealed the absence of four major lipoprotein families present in normal serum. 7. Lecithin—cholesterol acyltransferase activity of abetalipoproteinaemic serum was reduced to a value of about 50% of normal but lipoproteins of patients' serum could be utilized as a substrate only to a minor degree. The maximum activity was observed only if lipoproteins of normal serum were added to the assay system.

Decreased lecithin: Cholesterol acyltransferase activity in the plasma of hypercholesterolemic pigs

Lipids ◽  
1992 ◽  
Vol 27 (4) ◽  
pp. 266-269 ◽  
Author(s):  
Andras G. Lacko ◽  
S-M. Lee ◽  
Iraj Mirshahi ◽  
Judith Hasler-Rapacz ◽  
Bhalchandra J. Kudchodkar
Keyword(s):  
Cholesterol Acyltransferase ◽  
Acyltransferase Activity ◽  
Lecithin Cholesterol Acyltransferase
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