Agar dilution method for determination of antibiotic susceptibility of Ureaplasma urealyticum

1986 ◽  
Vol 5 (Supplement) ◽  
pp. S332-334 ◽  
Author(s):  
GEORGE E. KENNY ◽  
FRANK D. CARTWRIGHT ◽  
MARILYN C. ROBERTS
Keyword(s):  
Antibiotic Susceptibility ◽  
Ureaplasma Urealyticum ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Agar Dilution

Assessment of Antibiotic Susceptibility of Ureaplasma urealyticum from Prostitutes and Outpatient Clinic Patients Using the E-Test and Agar Dilution Method

Chemotherapy ◽  
2003 ◽  
Vol 49 (1-2) ◽  
pp. 39-43 ◽  
Author(s):  
Hüseyin Çakan ◽  
Erdal Polat ◽  
Bekir Kocazeybek ◽  
Pelin Öcal ◽  
İsmail Çepni ◽  
...  
Keyword(s):  
Outpatient Clinic ◽  
Antibiotic Susceptibility ◽  
Ureaplasma Urealyticum ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Agar Dilution

scholarly journals In Vitro Antifungal Activity of Polysulfides-Rich Essential Oil of Ferula Latisecta Fruits against Human Pathogenic Dermatophytes

2008 ◽  
Vol 3 (9) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Mehrdad Iranshahi ◽  
Abdolmajid Fata ◽  
Bahareh Emami ◽  
Bibi Mohadeseh Jalalzadeh Shahri ◽  
Bibi Sedigheh Fazly Bazzaz
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Agar Dilution ◽  
Sulfur Containing ◽  
Type Strains ◽  
In Vitro Antifungal Activity

The increase in dermatophytoses and the fact that some patients do not respond well to therapy make it necessary to find new antifungal agents. As part of our ongoing studies on medicinal plants from Iran, we studied antidermatophytic activities of Ferula latisecta essential oil, which had shown considerable antifungal activity in preliminary antimicrobial screening. Antifungal activity was evaluated by determination of MIC values using the agar dilution method on type strains of Candida albicans and dermatophytes. The composition of the oil was characterized by GC and GC/MS analyses. The essential oil was rich in polysulfides (75.2%) and exhibited good activity against Trichophyton rubrum and T. verrucosom for about three weeks, with a MIC value 96 μg/mL. The oil showed antifungal activity, especially against dermatophytes, and the activity is probably related to the sulfur-containing components of the oil. This study has identified that the polysulfides-rich essential oil of Ferula latisecta fruits has activity against a range of human pathogenic dermatophytes, justifying future clinical trials to validate its use as a therapeutic alternative for dermatophytosis.

scholarly journals In Vitro Antimicrobial Susceptibility Testing of Helicobacter felis, H. bizzozeronii, and H. salomonis

2005 ◽  
Vol 49 (7) ◽  
pp. 2997-3000 ◽  
Author(s):  
K. Van den Bulck ◽  
A. Decostere ◽  
I. Gruntar ◽  
M. Baele ◽  
B. Krt ◽  
...  
Keyword(s):  
Antimicrobial Agent ◽  
Antimicrobial Agents ◽  
Susceptibility Testing ◽  
Acquired Resistance ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Helicobacter Felis ◽  
Agar Dilution

ABSTRACT The susceptibilities of Helicobacter felis (15 strains), H. bizzozeronii (7 strains), and H. salomonis (3 strains) to 10 antimicrobial agents were investigated by determination of the MIC using the agar dilution method. No consistent differences were noticed between the different Helicobacter species, which were all highly susceptible to ampicillin, clarithromycin, tetracycline, tylosin, enrofloxacin, gentamicin, and neomycin, as demonstrated by low MICs. Higher MICs were obtained for lincomycin (up to 8 μg/ml) and spectinomycin (up to 4 μg/ml). Two H. felis strains showed a MIC of 16 μg/ml for metronidazole, suggesting acquired resistance to this antimicrobial agent.

scholarly journals Differences in the Molecular Epidemiology and Antibiotic Susceptibility of Clostridium difficile Isolates in Pediatric and Adult Patients

2016 ◽  
Vol 60 (8) ◽  
pp. 4896-4900 ◽  
Author(s):  
Larry K. Kociolek ◽  
Dale N. Gerding ◽  
James R. Osmolski ◽  
Sameer J. Patel ◽  
David R. Snydman ◽  
...  
Keyword(s):  
Clostridium Difficile ◽  
Antibiotic Susceptibility ◽  
Restriction Endonuclease Analysis ◽  
Adult Patients ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Content Type ◽  
Agar Dilution ◽  
Rising Incidence ◽  
Antibiotics Susceptibility

ABSTRACTThe rising incidence ofClostridium difficileinfections (CDIs) in adults is partly related to the global spread of fluoroquinolone-resistant strains, namely, BI/NAP1/027. Although CDIs are also increasingly diagnosed in children, BI/NAP1/027 is relatively uncommon in children. Little is known about the antibiotic susceptibility of pediatric CDI isolates.C. difficilewas cultured fromtcdB-positive stools collected from children diagnosed with CDI between December 2012 and December 2013 at an academic children's hospital. CDI isolates were grouped by restriction endonuclease analysis (REA). MICs were measured by agar dilution method for 7 antibiotics. Susceptibility breakpoints were based on guidelines from CLSI and/or the European Committee on Antimicrobial Susceptibility Testing (EUCAST). MICs and REA groupings ofC. difficileisolates from 74 adult patients (29 isolates underwent REA) from a temporally and geographically similar adult cohort were compared to those of pediatric isolates. Among 122 pediatric and 74 adult isolates, respectively, the rates of resistance were as follows: metronidazole, 0% and 0%; vancomycin, 0% and 8% (P= 0.003); rifaximin, 1.6% and 6.7% (P= 0.11); clindamycin, 18.9% and 25.3% (P= 0.29); and moxifloxacin, 2.5% and 36% (P= <0.0001). Only 1 of 122 (0.8%) BI/NAP1/027 isolates was identified among the children, compared to 9 of 29 (31%) isolates identified among the adults (P= <0.0001). The 3 moxifloxacin-resistant pediatric isolates were of REA groups BI and CF and a nonspecific group. The 2 rifaximin-resistant pediatric isolates were of REA groups DH and Y. The 21 clindamycin-resistant pediatric isolates were distributed among 9 REA groups (groups A, CF, DH, G, L, M, and Y and 2 unique nonspecific REA groups). These data suggest that a diverse array of relatively antibiotic-susceptibleC. difficilestrains predominate in a cohort of children with CDI compared to adults.

Screening of antifungal and antimycotoxigenic activity of plant phenolic extracts

2008 ◽  
Vol 1 (2) ◽  
pp. 139-146 ◽  
Author(s):  
M. dos Santos Oliveira ◽  
E. Badiale Furlong
Keyword(s):  
Culture Medium ◽  
Orange Peel ◽  
Inhibitory Effect ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Potato Peel ◽  
Fungal Development ◽  
Agar Dilution ◽  
Aflatoxin B

The antifungal and antimycotoxigenic activities of extracts from edible plants were tested by the agar dilution method using the growth diameter of Aspergillus flavus as response and the determination of aflatoxins B1 and B2 in the culture medium. On the 7th incubation day, the greatest fungal inhibitions were reached by the extracts from potato peel; rice and wheat; lemon peel and pulp; eggplant peel; orange peel and pulp; and apple pulp. After the 14-day incubation, the extracts from banana (30 µg phenol/ml agar), eggplant (30 µg phenol/ml agar), and potato (50 and 67 µg phenol/ml agar) pulp reduced the production of aflatoxin B1 by 3.2%/µg phenol/ml agar, 2.9%/µg phenol/ml agar, 1.8%/µg phenol/ml agar and 0.85%/µg phenol/ml agar, respectively, in relation to the control. The extracts from the other vegetables fully inhibited the synthesis of the mycotoxin. These results point to the studied plants and their residues as potential sources of phenolic compounds that may have an inhibitory effect on fungal development and the production of mycotoxins in food.

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