scholarly journals Modified agar dilution method for rapid antibiotic susceptibility testing of anaerobic bacteria.

1978 ◽  
Vol 13 (3) ◽  
pp. 383-388 ◽  
Author(s):  
C W Hanson ◽  
W J Martin
Keyword(s):  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Anaerobic Bacteria ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Antibiotic Susceptibility Testing ◽  
Agar Dilution

scholarly journals COLISTIN RESISTANCE IN CARBAPENEM-RESISTANT KLEBSIELLA PNEUMONIAE STRAINS

2017 ◽  
Vol 10 (9) ◽  
pp. 70
Author(s):  
Beena Hosdurg Bhaskar ◽  
Shalini Shenoy Mulki ◽  
Sangeeta Joshi ◽  
Ranjeeta Adhikari ◽  
Bhavana Malavalli Venkatesh
Keyword(s):  
Antibiotic Susceptibility ◽  
Antimicrobial Agents ◽  
Susceptibility Testing ◽  
Ethylenediaminetetraacetic Acid ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Antibiotic Susceptibility Testing ◽  
Carbapenem Resistant

Objective: There is an increasing use of colistin consequent to increase in the infections caused by carbapenem-resistant Klebsiella pneumoniae.The present study was conducted to determine the minimum inhibitory concentration (MIC) of colistin and the resistance pattern of colistin in carbapenem-resistant K. pneumoniae (CRKP) strains in our intensive care unit (ICU).Methods: Antibiotic susceptibility testing for other antimicrobial agents was done by Kirby-Bauer disk diffusion method. MIC of colistin was determined by agar dilution method. The results of antibiotic susceptibility testing were interpreted as per Clinical Laboratory Standard Institute guidelines 2016 and MIC of colistin were interpreted as per European Committee on Antimicrobial susceptibility testing. The carbapenem resistance was phenotypically detected by modified hodge test and imipenem/imipenem ethylenediaminetetraacetic acid disk method.Results: Out of 518 K. pneumoniae, 329 were resistant to carbapenems, and 91 isolates showed resistance to colistin. The MIC of colistin ranged between 4 and >512 ug/ml and MIC90 was 16 ug/L and MIC50 was 4 ug/ml. A majority of the colistin-resistant isolates were found in multidisciplinary ICU (85/91).Conclusion: The emergence of colistin-resistant strains is a major problem due to limited treatment options for infections caused by CRKP carbapenemase producing K. pneumoniae. Colistin should not be used alone, combination therapy should be preferred.

scholarly journals Cefotetan Susceptibility Testing Against Anaerobic Bacteria From Obstetrical and Gynecologic Sources: Comparison of Five Different Methods

1993 ◽  
Vol 1 (1) ◽  
pp. 23-26
Author(s):  
Maurizio Maccato ◽  
Gerald Riddle ◽  
Sebastian Faro
Keyword(s):  
Susceptibility Testing ◽  
Anaerobic Bacteria ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Gradient System ◽  
Minimal Inhibitory Concentrations ◽  
Gradient Technique ◽  
Doubling Dilution ◽  
Agar Dilution ◽  
Elution Method

Five different antibiotic susceptibility methods were utilized to test the effectiveness of cefotetan against 200 anaerobic bacteria recovered from patients with obstetrical or gynecological infections. The object of this study was to determine if a more economical and rapid method for anaerobic susceptibility testing was as acceptable as the reference agar dilution method. The five methods were: 1) broth disk elution, 2) microbroth technique, 3) a commercially available microbroth technique, 4) a commercially available spiral gradient technique, and 5) reference agar dilution. The minimal inhibitory concentrations (MICs) calculated from the spiral gradient technique were equal to or within one doubling dilution of the reference system in 99.5% of cases, while the percentage for the commercially available microbroth system was 96.8%, very similar to the microbroth technique used in our laboratory that yielded a percentage of 96.3. The disk elution method correlated to the reference agar dilution method in 95.3% cases. While the overall agreement between these techniques is good, especially for the spiral gradient system, clustering of certain organisms near the breakpoint of the antibiotic tested results in variability in the labeling of these organisms as susceptible or resistant. This problem appears to be particularly significant for the disk elution method. Therefore, further refinements in these methods of suscleptibility testing are needed in order to provide a more clinically useful assessment of the susceptibility or resistance of certain bacterial isolates.

scholarly journals Susceptibility Testing of Anaerobic Bacteria: Evaluation of the Redesigned (Version 96) bioMérieux ATB ANA Device

1999 ◽  
Vol 37 (6) ◽  
pp. 1824-1828 ◽  
Author(s):  
L. Dubreuil ◽  
I. Houcke ◽  
E. Singer
Keyword(s):  
Susceptibility Testing ◽  
Clinical Laboratory ◽  
Anaerobic Bacteria ◽  
Reference Method ◽  
National Committee ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
French Society ◽  
Antibiotic Susceptibilities ◽  
Agar Dilution

We compared the susceptibility results for 200 clinical anaerobes with nine antibiotics obtained by using a new ATB ANA (bioMérieux) device against those obtained by the National Committee for Clinical Laboratory Standards (NCCLS) standard agar dilution method. For better evaluation of the device, we added some resistant Bacteroides fragilis group strains from our own collection: 3, 6, and 12 strains that were resistant to imipenem, ticarcillin plus clavulanic acid, and co-amoxiclav, respectively, and 2 other strains with decreased susceptibility to metronidazole. For some strains that did not grow on ATB S medium, tests were performed by using West-Wilkins medium supplemented with 1.5% agar. The new ATB ANA device made clinical categorization of the investigated strains possible, according to French (Committee of the Antibiogram of the French Society of Microbiology) or U.S. (NCCLS) breakpoints, with the following respective results: category agreement, 94.3 and 94.9%; minor errors, 4.8 and 3.8%; major errors, 0.4 and 0.8%; and very major errors 4.6 and 4.2%. The ATB ANA device was able to detect low-level metronidazole-resistant B. fragilis strains according to the French breakpoints but not the NCCLS ones. For B. fragilis and β-lactamase-positive Prevotellastrains, the clustering effect of amoxicillin MICs around the French breakpoints led to more frequent minor errors. ATB ANA is a very convenient method to determine the antibiotic susceptibilities of anaerobes. Results obtained by ATB ANA correlated well with those obtained by the reference method.

scholarly journals Inaccuracy of the Disk Diffusion Method Compared with the Agar Dilution Method for Susceptibility Testing of Campylobacter spp.

2011 ◽  
Vol 50 (1) ◽  
pp. 52-56 ◽  
Author(s):  
Mirva Lehtopolku ◽  
Pirkko Kotilainen ◽  
Pauli Puukka ◽  
Ulla-Maija Nakari ◽  
Anja Siitonen ◽  
...  
Keyword(s):  
Susceptibility Testing ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Disk Diffusion Method ◽  
Agar Dilution ◽  
Campylobacter Spp

Improvement of a disk diffusion method for antibiotic susceptibility testing of anaerobic bacteria. French recommendations revisited for 2020

Anaerobe ◽  
2020 ◽  
Vol 64 ◽  
pp. 102213
Author(s):  
L. Dubreuil ◽  
F. Jehl ◽  
C. Cattoen ◽  
R. Bonnet ◽  
J.P. Bru ◽  
...  
Keyword(s):  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Anaerobic Bacteria ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Antibiotic Susceptibility Testing ◽  
Disk Diffusion Method

Agar dilution method for determination of antibiotic susceptibility of Ureaplasma urealyticum

1986 ◽  
Vol 5 (Supplement) ◽  
pp. S332-334 ◽  
Author(s):  
GEORGE E. KENNY ◽  
FRANK D. CARTWRIGHT ◽  
MARILYN C. ROBERTS
Keyword(s):  
Antibiotic Susceptibility ◽  
Ureaplasma Urealyticum ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Agar Dilution
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