Evaluation of Agar Dilution Method for Determination of Sensitivity of Bacteria to Antibiotics.

1953 ◽  
Vol 82 (4) ◽  
pp. 574-577 ◽  
Author(s):  
W. E. Schatten ◽  
R. F. Parker
Keyword(s):  
Dilution Method ◽  
Agar Dilution Method ◽  
Agar Dilution

scholarly journals In Vitro Antifungal Activity of Polysulfides-Rich Essential Oil of Ferula Latisecta Fruits against Human Pathogenic Dermatophytes

2008 ◽  
Vol 3 (9) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Mehrdad Iranshahi ◽  
Abdolmajid Fata ◽  
Bahareh Emami ◽  
Bibi Mohadeseh Jalalzadeh Shahri ◽  
Bibi Sedigheh Fazly Bazzaz
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Agar Dilution ◽  
Sulfur Containing ◽  
Type Strains ◽  
In Vitro Antifungal Activity

The increase in dermatophytoses and the fact that some patients do not respond well to therapy make it necessary to find new antifungal agents. As part of our ongoing studies on medicinal plants from Iran, we studied antidermatophytic activities of Ferula latisecta essential oil, which had shown considerable antifungal activity in preliminary antimicrobial screening. Antifungal activity was evaluated by determination of MIC values using the agar dilution method on type strains of Candida albicans and dermatophytes. The composition of the oil was characterized by GC and GC/MS analyses. The essential oil was rich in polysulfides (75.2%) and exhibited good activity against Trichophyton rubrum and T. verrucosom for about three weeks, with a MIC value 96 μg/mL. The oil showed antifungal activity, especially against dermatophytes, and the activity is probably related to the sulfur-containing components of the oil. This study has identified that the polysulfides-rich essential oil of Ferula latisecta fruits has activity against a range of human pathogenic dermatophytes, justifying future clinical trials to validate its use as a therapeutic alternative for dermatophytosis.

scholarly journals In Vitro Antimicrobial Susceptibility Testing of Helicobacter felis, H. bizzozeronii, and H. salomonis

2005 ◽  
Vol 49 (7) ◽  
pp. 2997-3000 ◽  
Author(s):  
K. Van den Bulck ◽  
A. Decostere ◽  
I. Gruntar ◽  
M. Baele ◽  
B. Krt ◽  
...  
Keyword(s):  
Antimicrobial Agent ◽  
Antimicrobial Agents ◽  
Susceptibility Testing ◽  
Acquired Resistance ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Helicobacter Felis ◽  
Agar Dilution

ABSTRACT The susceptibilities of Helicobacter felis (15 strains), H. bizzozeronii (7 strains), and H. salomonis (3 strains) to 10 antimicrobial agents were investigated by determination of the MIC using the agar dilution method. No consistent differences were noticed between the different Helicobacter species, which were all highly susceptible to ampicillin, clarithromycin, tetracycline, tylosin, enrofloxacin, gentamicin, and neomycin, as demonstrated by low MICs. Higher MICs were obtained for lincomycin (up to 8 μg/ml) and spectinomycin (up to 4 μg/ml). Two H. felis strains showed a MIC of 16 μg/ml for metronidazole, suggesting acquired resistance to this antimicrobial agent.

Screening of antifungal and antimycotoxigenic activity of plant phenolic extracts

2008 ◽  
Vol 1 (2) ◽  
pp. 139-146 ◽  
Author(s):  
M. dos Santos Oliveira ◽  
E. Badiale Furlong
Keyword(s):  
Culture Medium ◽  
Orange Peel ◽  
Inhibitory Effect ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Potato Peel ◽  
Fungal Development ◽  
Agar Dilution ◽  
Aflatoxin B

The antifungal and antimycotoxigenic activities of extracts from edible plants were tested by the agar dilution method using the growth diameter of Aspergillus flavus as response and the determination of aflatoxins B1 and B2 in the culture medium. On the 7th incubation day, the greatest fungal inhibitions were reached by the extracts from potato peel; rice and wheat; lemon peel and pulp; eggplant peel; orange peel and pulp; and apple pulp. After the 14-day incubation, the extracts from banana (30 µg phenol/ml agar), eggplant (30 µg phenol/ml agar), and potato (50 and 67 µg phenol/ml agar) pulp reduced the production of aflatoxin B1 by 3.2%/µg phenol/ml agar, 2.9%/µg phenol/ml agar, 1.8%/µg phenol/ml agar and 0.85%/µg phenol/ml agar, respectively, in relation to the control. The extracts from the other vegetables fully inhibited the synthesis of the mycotoxin. These results point to the studied plants and their residues as potential sources of phenolic compounds that may have an inhibitory effect on fungal development and the production of mycotoxins in food.

scholarly journals In Vitro Activities of Ceftobiprole, Tigecycline, Daptomycin, and 19 Other Antimicrobials against Methicillin-Resistant Staphylococcus aureus Strains from a National Survey of Belgian Hospitals

2006 ◽  
Vol 50 (8) ◽  
pp. 2680-2685 ◽  
Author(s):  
Olivier Denis ◽  
Ariane Deplano ◽  
Claire Nonhoff ◽  
Marie Hallin ◽  
Raf De Ryck ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Agents ◽  
Therapeutic Potential ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Methicillin Resistant ◽  
Excellent Activity ◽  
Agar Dilution

ABSTRACT The in vitro activities of 22 antimicrobial agents, including ceftobiprole, daptomycin, and tigecycline, against 511 methicillin-resistant Staphylococcus aureus (MRSA) isolates from 112 Belgian hospitals were studied by using the CLSI agar dilution method. Isolates were characterized by pulsed-field gel electrophoresis (PFGE) analysis and by PCR detection of determinants of resistance to aminoglycosides, macrolides-lincosamides-streptogramins, and tetracyclines. A representative set of isolates with different PFGE genotypes was further characterized by multilocus sequence typing, determination of staphylococcal cassette chromosome mec (SCCmec) type, and multiplex PCR for toxic shock syndrome type 1 (TSST-1) and Panton-Valentine leukocidin genes. MRSA isolates belonged to nine epidemic MRSA clones, of which sequence type 45 (ST45)-SCCmec IV and ST8-SCCmec IV were predominant, accounting for 49 and 20% of isolates, respectively. The distribution of antimicrobial resistance and TSST-1 genes was strongly linked to clonal types. Ceftobiprole, daptomycin, and tigecycline showed high activity against all isolates of these sporadic and epidemic MRSA clones, as indicated by MIC90s of 2 mg/liter, 0.5 mg/liter, and 0.25 mg/liter, respectively. The MIC distribution of daptomycin and tigecycline was not different in isolates with decreased susceptibility to glycopeptides or tetracyclines, respectively. Ceftobiprole MICs were not correlated with oxacillin and cefoxitin MICs. These data indicate excellent activity of the newly developed agents ceftobiprole, daptomycin, and tigecycline against MRSA isolates recently recovered from hospitalized patients in Belgium, supporting their therapeutic potential for nosocomial MRSA infections.

Sign in / Sign up

Export Citation Format

Share Document