Interrater Variability in Diagnosis of Cervical Biopsies from Women with HIV-1: Results from the Womenʼs Interagency HIV Study

2000 ◽  
Vol 4 (4) ◽  
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Lynn Kirstein ◽  
Teresa Darragh ◽  
Pincas Bitterman ◽  
Mary Sidawy ◽  
...  
2000 ◽  
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Pincas Bitterman ◽  
Mary Sidawy ◽  
...  

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Howard Minkoff

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Howard Minkoff ◽  
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Salih Yasin ◽  
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AIDS ◽  
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Angela M. Caliendo ◽  
...  

Background. Fastidious bacteria have been associated with bacterial vaginosis (BV) using PCR methods. We assessed the prevalence of these bacteria in HIV-1 infected women and their relationship with vaginal pH and shedding of HIV-1 RNA.Methods. 64 cervicovaginal lavage (CVL) samples were collected from 51 women. Vaginal microbiota were characterized using 8 bacterium-specific quantitative PCR assays.Results. Women with the fastidious bacteria Bacterial Vaginosis Associated Bacterium (BVAB) 1, 2, and 3 showed a trend to increased HIV-1 shedding (OR 2.59–3.07,P=.14–.17). Absence ofLactobacillus crispatus(P<.005) and presence of BVAB2 (P<.001) were associated with elevated vaginal pH. BVAB1, 2, and 3 were highly specific indicators of BV in HIV-infected women, with specificities of 89%–93%.Conclusions. Fastidious bacteria (BVAB 1, 2, and 3) remain specific indicators of BV in HIV-infected women, and BVAB2 may contribute to the elevated vaginal pH that is a hallmark of this syndrome.


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