Characterization of a Defective Form of Tomato Spotted Wilt Virus

ABSTRACT Tomato spotted wilt virus (TSWV), a member of the Tospovirus genus within the Bunyaviridae, is an economically important plant pathogen with a worldwide distribution. TSWV is transmitted to plants via thrips (Thysanoptera: Thripidae), which transmit the virus in a persistent propagative manner. The envelope glycoproteins, GN and GC, are critical for the infection of thrips, but they are not required for the initial infection of plants. Thus, it is assumed that the envelope glycoproteins play important roles in the entry of TSWV into the insect midgut, the first site of infection. To directly test the hypothesis that GN plays a role in TSWV acquisition by thrips, we expressed and purified a soluble, recombinant form of the GN protein (GN-S). The expression of GN-S allowed us to examine the function of GN in the absence of other viral proteins. We detected specific binding to thrips midguts when purified GN-S was fed to thrips in an in vivo binding assay. The TSWV nucleocapsid protein and human cytomegalovirus glycoprotein B did not bind to thrips midguts, indicating that the GN-S-thrips midgut interaction is specific. TSWV acquisition inhibition assays revealed that thrips that were concomitantly fed purified TSWV and GN-S had reduced amounts of virus in their midguts compared to thrips that were fed TSWV only. Our findings that GN-S binds to larval thrips guts and decreases TSWV acquisition provide evidence that GN may serve as a viral ligand that mediates the attachment of TSWV to receptors displayed on the epithelial cells of the thrips midgut.

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Identification and characterization of host proteins interacting with NSm, the Tomato spotted wilt virus movement protein

Plant Protection Science ◽

10.17221/10331-pps ◽

2002 ◽

Vol 38 (SI 1 - 6th Conf EFPP 2002) ◽

pp. S108-S111

Author(s):

M. Paape ◽

S. Nell ◽

S. von Bargen ◽

J.-W. Kellmann

Keyword(s):

Tomato Spotted Wilt Virus ◽

Movement Protein ◽

Host Proteins ◽

Tomato Spotted Wilt ◽

J Domain ◽

Wilt Virus ◽

Identification And Characterization ◽

Two Hybrid ◽

Interaction Trap

To search for host proteins involved in systemic spreading of Tomato spotted wilt virus (TSWV), the virus-encoded NSm movement protein has been utilized as a bait in yeast two-hybrid interaction trap assays. J-domain chaperones from different host species and a protein denominated At-4/1 from Arabidopsis thaliana showing homologies to myosins and kinesins were identified as NSm-interacting partners. In this communication we illustrate that following TSWV infection, J-domain proteins accumulated in systemically infected leaves of A. thaliana, whereas At-4/1 was constitutively detected in leaves of A. thaliana and Nicotiana rustica.

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Molecular Characterization of Tomato Spotted Wilt Virus Defective Interfering RNAs and Detection of Truncated L Proteins

Virology ◽

10.1006/viro.1998.9271 ◽

1998 ◽

Vol 248 (2) ◽

pp. 342-356 ◽

Cited By ~ 14

Author(s):

Alice K. Inoue-Nagata ◽

Richard Kormelink ◽

Jean-Yves Sgro ◽

Tatsuya Nagata ◽

Elliot W. Kitajima ◽

...

Keyword(s):

Molecular Characterization ◽

Tomato Spotted Wilt Virus ◽

Tomato Spotted Wilt ◽

L Proteins ◽

Wilt Virus

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Characterization of the Nucleic Acid Binding Properties of Tomato Spotted Wilt Virus Nucleocapsid Protein

Virology ◽

10.1006/viro.1998.9223 ◽

1998 ◽

Vol 248 (1) ◽

pp. 6-11 ◽

Cited By ~ 54

Author(s):

K.E. Richmond ◽

K. Chenault ◽

J.L. Sherwood ◽

T.L. German

Keyword(s):

Nucleic Acid ◽

Nucleocapsid Protein ◽

Tomato Spotted Wilt Virus ◽

Nucleic Acid Binding ◽

Binding Properties ◽

Tomato Spotted Wilt ◽

Wilt Virus

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Status of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolates

Acta Virologica ◽

10.4149/av_2011_04_337 ◽

2012 ◽

Vol 55 (04) ◽

pp. 337-347 ◽

Cited By ~ 8

Author(s):

I. STANKOVIĆ ◽

A. BULAJIĆ ◽

A. VUČUROVIĆ ◽

D. RISTIĆ ◽

K. MILOJEVIĆ ◽

...

Keyword(s):

Molecular Characterization ◽

Tomato Spotted Wilt Virus ◽

Tomato Spotted Wilt ◽

Wilt Virus ◽

Virus Isolates

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Variability in Field Response of Peanut Genotypes from the U.S. and China to Tomato Spotted Wilt Virus and Leaf Spots

Peanut Science ◽

10.3146/ps11-6.1 ◽

2012 ◽

Vol 39 (1) ◽

pp. 30-37 ◽

Cited By ~ 9

Author(s):

Yan Li ◽

Albert K. Culbreath ◽

Charles Y. Chen ◽

Steve J. Knapp ◽

C. Corley Holbrook ◽

...

Keyword(s):

Leaf Spot ◽

Tomato Spotted Wilt Virus ◽

Field Trials ◽

Breeding Lines ◽

Leaf Spots ◽

Tomato Spotted Wilt ◽

Total Incidence ◽

Wilt Virus ◽

Selection Of

Abstract Tomato spotted wilt, caused by Tomato spotted wilt virus (TSWV) and transmitted by thrips, and early leaf spot and late leaf spot are among the most important diseases of peanut in the southeastern United States. The objective of this study was to compare field susceptibility of diverse peanut lines to TSWV and leaf spot pathogens for selection of lines for mapping population development. In field trials in 2007 and 2008, 22 genotypes were evaluated for reactions to TSWV and leaf spots. Early leaf spot was the predominate pathogen in both years. There was a near-continuous range of spotted wilt from 18% to 79% for the total incidence rating with any symptoms caused by TSWV. In general, NC94022, ‘Georganic’, C689-6-2, ‘Georgia-01R’, C724-19-25, TifGP-1, C11-154-61, C12-3-114-58, and ‘Tifguard’ were among the most resistant genotypes to TSWV, whereas GT-C20, GT-C9 and PE-2 were the most susceptible. Final percentage of defoliation by leaf spots ranged from 10% to 97% for both years. Genotypes C689-2, Georgia-01R, C12-3-114-58, C11-154-61, Tifguard and Georganic showed resistance to leaf spots, whereas ‘NC-6’, ‘Spancross’, GT-C9, GT-C20 and PE-2 were susceptible to leaf spots. There were 3 cultivars and 3 breeding lines classified as resistant to both TSWV and leaf spots; and there were 3 genotypes from China susceptible to both TSWV and leaf spots. These phenotypic disease reaction data can be used in conjunction with genetic characterization of these genotypes for development of recombinant inbred line populations in efforts to develop markers for resistance to TSWV and leaf spots.

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