Human neutralizing human immunodeficiency virustype 2-specific Fab molecules generated by phage display

A panel of human immunodeficiency virus type 2 (HIV-2)-neutralizing, recombinant Fab fragments was generated by using the phage display technique. The combinatorial library was derived from an asymptomatic, HIV-2-seropositive individual and constructed on the surface of filamentous phage by using the pComb3 phagemid vector and then screened against native HIV-2 envelope glycoprotein (gp125). Ten of 30 Fab fragments generated displayed strong reactivity in an ELISA and were therefore selected for further study. Six of these possessed neutralizing capacity, with titres varying from 20 to 80 against the homologous HIV-2 strain, and one also had a weak neutralizing capacity against a heterologous HIV-2 isolate, K135. Sequencing of the heavy chain CDR3 regions showed that the gp125-specific Fabs represented individual clones. These reagents may be useful for studies on the conformational structures of the HIV-2 envelope antigens and their immunogenicity, which may help in vaccine design. Furthermore, the cloned Fab genes may be transformed into whole IgG for eukaryotic expression, and as such used for therapeutic and immunoprophylactic studies in HIV-2-infected macaques and, possibly, for human immunoprophylaxis against HIV-2.

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Specific Fab fragments recovered by phage display technique recognizing human spermatozoa

International Journal of Andrology ◽

10.1111/j.1365-2605.2008.00876.x ◽

2009 ◽

Vol 32 (5) ◽

pp. 442-452 ◽

Cited By ~ 3

Author(s):

Dorota Fiszer ◽

Małgorzata Pupecka ◽

Katarzyna Schmidt ◽

Natalia Rozwadowska ◽

Marzena Kamieniczna ◽

...

Keyword(s):

Phage Display ◽

Human Spermatozoa ◽

Fab Fragments ◽

Phage Display Technique ◽

Display Technique

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The phage display technique and transfusion medicine

Transfusion ◽

10.1046/j.1537-2995.2001.41010001.x ◽

2001 ◽

Vol 41 (1) ◽

pp. 1-3 ◽

Cited By ~ 3

Author(s):

Petr Jarolim

Keyword(s):

Phage Display ◽

Transfusion Medicine ◽

Phage Display Technique ◽

Display Technique

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Development of Human Antibody Inhibiting RNase H Activity of Polymerase of Hepatitis B Virus Using Phage Display Technique

Immune Network ◽

10.4110/in.2004.4.1.16 ◽

2004 ◽

Vol 4 (1) ◽

pp. 16

Author(s):

Seong-Rak Lee ◽

Eun-Kyoung Song ◽

Young-Joo Jeong ◽

Young-Yi Lee ◽

Ik-Jung Kim ◽

...

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Phage Display ◽

Rnase H ◽

Human Antibody ◽

Phage Display Technique ◽

B Virus ◽

Display Technique

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A Novel Method forin SituDetection of Hydrolyzable Casein Fragments in a Cheese Matrix by Antibody Phage Display Technique and CLSM

Journal of Agricultural and Food Chemistry ◽

10.1021/jf901026m ◽

2009 ◽

Vol 57 (21) ◽

pp. 10049-10054 ◽

Cited By ~ 3

Author(s):

Zhi Duan ◽

Dagmar Adeline Brüggemann ◽

Henrik Siegumfeldt

Keyword(s):

Phage Display ◽

Phage Display Technique ◽

Antibody Phage ◽

Antibody Phage Display ◽

Novel Method ◽

Display Technique

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Phage-based Nanomedicines as New Immune Therapeutic Agents for Breast Cancer

Current Pharmaceutical Design ◽

10.2174/1381612824666180327152117 ◽

2018 ◽

Vol 24 (11) ◽

pp. 1195-1203 ◽

Cited By ~ 3

Author(s):

Atefeh Arab ◽

Rezvan Yazdian Robati ◽

Jessica Nicastro ◽

Roderick Slavcev ◽

Javad Behravan

Keyword(s):

Breast Cancer ◽

Phage Display ◽

Biomedical Applications ◽

Therapeutic Agents ◽

Molecular Method ◽

Specific Antibodies ◽

Phage Display Technique ◽

Specific Antigens ◽

Protein Libraries ◽

Display Technique

Despite years of investigation, breast cancer remains a major cause of death worldwide. Phage display is a powerful molecular method in which peptide and protein libraries can be displayed via genetic fusions on the surface of phages. This approach has tremendous potential for biomedical applications and has already facilitated the discovery of specific antibodies, specific antigens, and peptides with potential roles in the diagnosis and treatment of malignancies including breast cancer. In this review, we discuss the new and the latest advancements in the applications of the phage display technique in the provision of immune therapeutics for breast cancer.

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