Salinisphaera halophila sp. nov., a moderately halophilic bacterium isolated from brine of a salt well

2012 ◽  
Vol 62 (Pt_9) ◽  
pp. 2174-2179 ◽  
Author(s):  
Yun-Jiao Zhang ◽  
Shu-Kun Tang ◽  
Rong Shi ◽  
Hans-Peter Klenk ◽  
Chao Chen ◽  
...  
Keyword(s):  
Type Species ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Content Type ◽  
Moderately Halophilic ◽  
Link Type ◽  
Moderately Halophilic Bacterium ◽  
Sequence Similarities

A Gram-stain-negative, moderately halophilic, strictly aerobic bacterium, designated YIM 95161T, was isolated from brine of a salt well in Yunnan province, China, and subjected to a taxonomic study using a polyphasic approach. Cells of strain YIM 95161T were short rods, approximately 0.9–1.4 µm long and 0.4–0.6 µm wide. Strain YIM 95161T grew at 15–40 °C (optimum, 25–30 °C), 6–29 % (w/v) NaCl (optimum, 14–19 %) and at pH 5.0–8.0 (optimum, pH 7.0). The predominant isoprenoid quinone was Q-8. The major fatty acids (>10 %) were summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c) and C14 : 0. The polar lipids consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unknown phosphoglycolipid and two unknown lipids. The DNA G+C content was 69.5 %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 95161T was a member of the genus Salinisphaera and exhibited sequence similarities of 96.7 %, 95.6 % and 95.4 % to Salinisphaera shabanensis E1L3AT, Salinisphaera dokdonensis CL-ES53T and Salinisphaera hydrothermalis EPR70T, respectively. On the basis of phylogenetic, physiological and chemotaxonomic analysis, strain YIM 95161T represents a novel species of the genus Salinisphaera , for which the name Salinisphaera halophila sp. nov. is proposed. The type strain is YIM 95161T ( = CCTCC AB 2011132T = JCM 17431T).

scholarly journals Aquisalimonas lutea sp. nov., a moderately halophilic bacterium from a saltern

2015 ◽  
Vol 65 (Pt_4) ◽  
pp. 1354-1359 ◽  
Author(s):  
Carmen Infante-Domínguez ◽  
Cristina Sánchez-Porro ◽  
Antonio Ventosa
Keyword(s):  
Dna Hybridization ◽  
Type Species ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Content Type ◽  
Moderately Halophilic ◽  
Link Type ◽  
Moderately Halophilic Bacterium

A yellow-pigmented, motile, Gram-stain-negative, moderately halophilic and strictly aerobic bacterium, designated BA42AL-1T, was isolated from water of a saltern of Santa Pola, Alicante, Spain. Strain BA42AL-1T grew in media containing 5–20 % (w/v) salts (optimum 7.5 % salts). It grew between pH 6.0 and 9.0 (optimally at pH 7.5) and at 15–45 °C (optimally at 37 °C). Phylogenetic analysis based on the comparison of 16S rRNA gene sequences revealed that strain BA42AL-1T is a member of the genus Aquisalimonas . The closest relatives to this strain were Aquisalimonas halophila YIM 95345T and Aquisalimonas asiatica CG12T with sequence similarities of 99.4 % and 97.0 %, respectively. DNA–DNA hybridization between the novel isolate and Aquisalimonas halophila YIM 95345T revealed a relatedness of 54 %. The major fatty acids of strain BA42AL-1T were C18 : 1ω6c/C18 : 1ω7c, C19 : 0 cyclo ω8c and C16 : 0, and lower contents of C12 : 0 and C18 : 0. The polar lipid pattern of strain BA42AL-1T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine, two glycolipids, a lipid and four unknown phospholipids. The G+C content of the genomic DNA of this strain was 65.0 mol%. Based on the DNA–DNA hybridization, phenotypic, chemotaxonomic and phylogenetic data presented in this study, strain BA42AL-1T is proposed as a novel species of the genus Aquisalimonas , for which the name Aquisalimonas lutea sp. nov. is suggested. The type strain is BA42AL-1T ( = CCM 8472T = CECT 8326T = LMG 27614T).

Ornithinibacillus halophilus sp. nov., a moderately halophilic, Gram-stain-positive, endospore-forming bacterium from a hypersaline lake

2013 ◽  
Vol 63 (Pt_3) ◽  
pp. 844-848 ◽  
Author(s):  
Maryam Bagheri ◽  
Mohammad Ali Amoozegar ◽  
Peter Schumann ◽  
Maryam Didari ◽  
Malihe Mehrshad ◽  
...  
Keyword(s):  
Type Species ◽  
Halophilic Bacterium ◽  
Hypersaline Lake ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Gram Stain ◽  
Content Type ◽  
Moderately Halophilic ◽  
Link Type ◽  
Moderately Halophilic Bacterium

A novel Gram-stain-positive, moderately halophilic bacterium, designated strain G8BT, was isolated from water of the hypersaline lake Aran-Bidgol in Iran and characterized taxonomically using a polyphasic approach. Cells of strain G8BT were rod-shaped, motile and produced oval endospores at a terminal position in swollen sporangia. Strain G8BT was strictly aerobic, catalase-positive and oxidase-negative. The strain was able to grow at NaCl concentrations of 0.5–12.5 % (w/v), with optimum growth occurring at 5–7.5 % (w/v) NaCl. The optimum temperature and pH for growth were 35–40 °C and pH 7.5–8.0, respectively. On the basis of 16S rRNA gene sequence analysis, strain G8BT was shown to belong to the genus Ornithinibacillus within the phylum Firmicutes and showed closest phylogenetic similarity with Ornithinibacillus bavariensis WSBC 24001T (97.6 %). The DNA G+C content of strain G8BT was 36.9 mol%. The major cellular fatty acids of strain G8BT were anteiso-C15 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C16 : 0, and its polar lipid pattern consisted of phosphatidylglycerol, diphosphatidylglycerol, four unknown phospholipids and an unknown aminolipid. The isoprenoid quinones were MK-7 (98 %) and MK-8 (2 %). Strain G8BT contained a peptidoglycan of type A4β, l-Orn–d-Asp. All these features confirmed the placement of isolate G8BT within the genus Ornithinibacillus . DNA–DNA hybridization experiments revealed a low level of relatedness (6 %) between strain G8BT and Ornithinibacillus bavariensis DSM 15681T. On the basis of evidence from this study, a novel species of the genus Ornithinibacillus , Ornithinibacillus halophilus sp. nov., is proposed, with strain G8BT ( = IBRC-M 10683T = KCTC 13822T) as the type strain.

scholarly journals Fodinicurvata halophila sp. nov., a moderately halophilic bacterium from a marine saltern

2015 ◽  
Vol 65 (Pt_3) ◽  
pp. 766-771 ◽  
Author(s):  
Carmen Infante-Dominguez ◽  
Paul A. Lawson ◽  
Crystal N. Johnson ◽  
Cristina Sánchez-Porro ◽  
Antonio Ventosa
Keyword(s):  
Type Species ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
The Novel ◽  
Content Type ◽  
Moderately Halophilic ◽  
Link Type ◽  
Moderately Halophilic Bacterium ◽  
Lipid Pattern ◽  
Sequence Similarities

A Gram-stain-negative, rod-shaped, facultatively anaerobic, moderately halophilic bacterium, designated strain BA45ALT, was isolated from water of a saltern located in Santa Pola, Alicante, Spain. Cells were motile, and catalase- and oxidase-positive. Strain BA45ALT grew at temperatures in the range 14–45 °C (optimally at 37 °C), at pH 5.0–9.0 (optimally at pH 7.5), and in media containing 5–20 % (w/v) salts [optimally in media containing 10 % (w/v) salts]. Phylogenetic analysis based on the comparison of 16S rRNA gene sequences revealed that strain BA45ALT is a member of the genus Fodinicurvata . The closest relatives to the novel strain were Fodinicurvata fenggangensis YIM D812T and Fodinicurvata sediminis YIM D82T with sequence similarities of 98.2 % and 97.4 %, respectively. DNA–DNA hybridization between the novel isolate and these phylogenetically related species revealed relatedness values of 30 % and 15 %, respectively, with respect to the aforementioned species. The major cellular fatty acids of strain BA45ALT were C18 : 1ω7c, C16 : 0 and iso-C15 : 0. The G+C content of the genomic DNA of strain BA45ALT was 58.0 mol%, and the polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine and a number of unknown phospholipids and lipids. Based on phenotypic, chemotaxonomic and phylogenetic data presented in this study, strain BA45ALT constituted a novel species of the genus Fodinicurvata , for which the name Fodinicurvata halophila sp. nov. is suggested. The type strain is BA45ALT ( = CCM 8504T = CECT 8472T = JCM 19075T = LMG 27945T).

scholarly journals Gracilibacillus bigeumensis sp. nov., a moderately halophilic bacterium from solar saltern soil

2012 ◽  
Vol 62 (Pt_8) ◽  
pp. 1857-1863 ◽  
Author(s):  
Pankyung Kim ◽  
Jae-Chan Lee ◽  
Dong-Jin Park ◽  
Kee-Sun Shin ◽  
Jeong-Yoon Kim ◽  
...  
Keyword(s):  
Type Species ◽  
Halophilic Bacterium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Solar Saltern ◽  
Content Type ◽  
Moderately Halophilic ◽  
Link Type ◽  
Moderately Halophilic Bacterium

A Gram-staining-positive, moderately halophilic bacterium, designated strain BH097T, was isolated from solar saltern soil of Bigeum Island in south-west Korea. Cells were motile rods, producing spherical endospores at a terminal position in swollen sporangia. Strain BH097T was strictly aerobic, grew at pH 5.5–9.5 (optimum, pH 8.0), at 10–52 °C (optimum, 37 °C) and at salinities of 1–22 % (w/v) NaCl (optimum, 7 % NaCl). On the basis of 16S rRNA gene sequence analysis, strain BH097T was shown to belong to the genus Gracilibacillus within the phylum Firmicutes , and showed closest sequence similarity to Gracilibacillus saliphilus DSM 19802T (95.8 %), Gracilibacillus thailandensis TP2-8T (95.6 %), Gracilibacillus boraciitolerans DSM 17256T (95.5 %), ‘Gracilibacillus quinghaiensis’ DSM 17858 (95.4 %) and Gracilibacillus halophilus DSM 17856T (95.2 %). The DNA G+C content of this novel isolate was 37.9 mol%. The major cellular fatty acids of strain BH097T were anteiso-C15 : 0, iso-C15 : 0 and C16 : 0, and its polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol two unknown phospholipids and a glycolipid. The isoprenoid quinone was MK-7, and the peptidoglycan type was A1γ, with meso-diaminopimelic acid as the diagnostic diamino acid. On the basis of polyphasic evidence from this study, strain BH097T represents a novel species of the genus Gracilibacillus for which the name Gracilibacillus bigeumensis sp. nov. is proposed. The type strain is BH097T ( = KCTC 13130T = DSM 19028T).

Halobacillus fulvus sp. nov., a moderately halophilic bacterium isolated from shrimp paste (Ka-pi) in Thailand

2021 ◽  
Vol 71 (11) ◽  
Author(s):  
Auttaporn Booncharoen ◽  
Wonnop Visessanguan ◽  
Nattakorn Kuncharoen ◽  
Supalurk Yiamsombut ◽  
Pannita Santiyanont ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Type Species ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Content Type ◽  
Moderately Halophilic ◽  
Link Type ◽  
Moderately Halophilic Bacterium

An aerobic, Gram-stain-positive, endospore-forming, rod-shaped and moderately halophilic strain SKP4-6T, was isolated from shrimp paste (Ka-pi) collected from Samut Sakhon Province, Thailand. Phylogenetic analysis revealed that strain SKP4-6T belonged to the genus Halobacillus and was most closely related to Halobacillus salinus JCM 11546T (98.6 %), Halobacillus locisalis KCTC 3788T (98.6 %) and Halobacillus yeomjeoni KCTC 3957T (98.6 %) based on 16S rRNA gene sequence similarity. The digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain SKP4-6T and its related species were 18.2–19.3 % and 69.84–84.51 %, respectively, which were lower than the threshold recommended for species delineation. The strain grew optimally at 30–40 °C, at pH 7.0 and with 10–15 % (w/v) NaCl. It contained l-Orn–d-Asp in the cell wall peptidoglycan. The DNA G+C content was 44.8 mol%. The major fatty acids were iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The predominant isoprenoid quinone was MK-7. Phosphatidylglycerol and diphosphatidylglycerol were present as major polar lipids. Based on this polyphasic approach, digital DNA–DNA relatedness and ANI values, strain SKP4-6T represents a novel species of the genus Halobacillus , for which the name Halobacillus fulvus sp. nov. is proposed. The type strain is SKP4-6T (=JCM 32624T=TISTR 2595T).

scholarly journals Halomonas icarae sp. nov., a moderately halophilic bacterium isolated from beach soil in India

2020 ◽  
Author(s):  
Kuppusamy Pandiyan ◽  
Prity Kushwaha ◽  
Samadhan Y. Bagul ◽  
Hillol Chakdar ◽  
Munusamy Madhaiyan ◽  
...  
Keyword(s):  
16S Rrna ◽  
Type Species ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Aerobic Bacterium ◽  
Content Type ◽  
Moderately Halophilic ◽  
Link Type ◽  
Moderately Halophilic Bacterium

A moderately halophilic, Gram-stain-negative, aerobic bacterium, strain D1-1T, belonging to the genus Halomonas , was isolated from soil sampled at Pentha beach, Odisha, India. Phylogenetic trees reconstructed based on 16S rRNA genes and multilocus sequence analysis of gyrB and rpoD genes revealed that strain D1-1T belonged to the genus Halomonas and was most closely related to Halomonas alimentaria YKJ-16T (98.1 %) followed by Halomonas ventosae Al12T (97.5 %), Halomonas sediminicola CPS11T (97.5 %), Halomonas fontilapidosi 5CRT (97.4 %) and Halomonas halodenitrificans DSM 735T (97.2 %) on the basis of 16S rRNA gene sequence similarity. Sequence identities with other species within the genus were lower than 97.0 %. The digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values of 22.4–30 % and 79.5–85.4 % with close relatives of H. halodenitrificans DSM 735T, H . alimentaria YKJ-16T, H. ventosae Al12T and H. fontilapidosi 5CRT were lower than the threshold recommended for species delineation (70 % and 95–96 % for dDDH and ANI, respectively). Further, strain D1-1T formed yellow-coloured colonies; cells were rod-shaped, motile with optimum growth at 30 °C (range, 4–45 °C) and 2–8 % NaCl (w/v; grew up to 24 % NaCl). The major fatty acids were summed feature 8 (C18 : 1  ω7c/C18 : 1  ω6c), summed feature 3 (C16 : 1  ω7c/C16 : 1  ω6c) and C16 : 0 and the main respiratory quinone was ubiquinone Q-9 in line with description of the genus. Based on its chemotaxonomic and phylogenetic characteristics and genome uniqueness, strain D1-1T represents a novel species in the genus Halomonas , for which we propose the name Halomonas icarae sp. nov., within the family Halomonadaceae . The type strain is D1-1T (=JCM 33602T=KACC 21317T=NAIMCC-B-2254T).

scholarly journals Flaviflexus ciconiae sp. nov., isolated from the faeces of the oriental stork, Ciconia boyciana

2020 ◽  
Vol 70 (10) ◽  
pp. 5439-5444 ◽  
Author(s):  
Jae-Yun Lee ◽  
Woorim Kang ◽  
Pil Soo Kim ◽  
So-Yeon Lee ◽  
Na-Ri Shin ◽  
...  
Keyword(s):  
Type Species ◽  
Gene Sequence ◽  
Novel Species ◽  
Optimal Growth ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Content Type ◽  
Link Type ◽  
Sequence Similarities

A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48T, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea. Optimal growth of strain H23T48T was observed at 30–37 °C, pH 8 and with 3 % (w/v) NaCl. 16S rRNA gene sequence-based phylogenetic analysis revealed that strain H23T48T was closely related to the genus Flaviflexus , with 97.0 and 96.7 % sequence similarities to Flaviflexus salsibiostraticola EBR4-1-2T and Flaviflexus huanghaiensis H5T, respectively. Strain H23T48T possessed MK-9(H4) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1  ω9c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids. The polar lipids included phosphatidylglycerol, two unidentified lipids, six unidentified phospholipids and two unidentified glycophospholipids. The amino acid composition of the cell-wall peptidoglycan was l-alanine, l-lysine, d-glutamic acid, l-aspartic acid and glycine. The genomic G+C content of strain H23T48T is 59.5 mol% and the average nucleotide identity value between H23T48T and F. salsibiostraticola KCT C33148T (=EBR4-1-2T) is 75.5 %. Based on the obtained data, strain H23T48T represents a novel species of the genus Flaviflexus , for which the name Flaviflexus ciconiae sp. nov. is proposed. The type strain is H23T48T (=KCTC 49253T=JCM 33282T).

scholarly journals Aquisalimonas halophila sp. nov., a moderately halophilic bacterium isolated from a hypersaline mine

2014 ◽  
Vol 64 (Pt_7) ◽  
pp. 2210-2216 ◽  
Author(s):  
Yun-Jiao Zhang ◽  
Man Jia ◽  
Yong-Chun Ma ◽  
Kai-Yang Lu ◽  
Fei Tian ◽  
...  
Keyword(s):  
Type Strain ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Content Type ◽  
Moderately Halophilic ◽  
Link Type ◽  
Moderately Halophilic Bacterium

A Gram-negative, moderately halophilic, strictly aerobic strain, designated YIM 95345T, was isolated from a soil sample of a hypersaline mine in Yunnan province, PR China, and subjected to a polyphasic taxonomic study. Strain YIM 95345T grew at 15–45 °C (optimum 30–35 °C), 3.0–23.0 % (w/v) NaCl (optimum 10.0–11.0 %, w/v) and pH 6.0–9.0 (optimum pH 7.0–8.0). Phylogenetic analyses based on 16S rRNA gene sequences revealed that the organism belongs to the genus Aquisalimonas and exhibited sequence similarity of 96.6 % to the sole type strain Aquisalimonas asiatica CG12T. The predominant isoprenoid quinone was Q-8 and the major fatty acids were C16 : 0, C19 : 0 cyclo ω8c and C18 : 1ω7c. The polar lipids consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, three aminolipids and three unidentified phospholipids. The G+C content of the genomic DNA was 59.4 mol%. Based on the results of our comparative phylogenetic, chemotaxonomic and physiological analyses, the new isolate is assigned to a novel species of the genus Aquisalimonas , for which the name Aquisalimonas halophila sp. nov. is proposed, with the type strain YIM 95345T ( = DSM 25902T = CCTCC AB 2012043T).

Bacillus persicus sp. nov., a halophilic bacterium from a hypersaline lake

2013 ◽  
Vol 63 (Pt_4) ◽  
pp. 1229-1234 ◽  
Author(s):  
Maryam Didari ◽  
Mohammad Ali Amoozegar ◽  
Maryam Bagheri ◽  
Maliheh Mehrshad ◽  
Peter Schumann ◽  
...  
Keyword(s):  
Type Species ◽  
Halophilic Bacterium ◽  
Diaminopimelic Acid ◽  
Hypersaline Lake ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Content Type ◽  
Link Type ◽  
Slightly Halophilic Bacterium

A novel Gram-positive, slightly halophilic bacterium, designated strain B48T, was isolated from soil around the hypersaline lake Aran-Bidgol in Iran and characterized taxonomically using a polyphasic approach. Cells of strain B48T were non-motile rods and produced ellipsoidal endospores at a central or subterminal position in swollen sporangia. Strain B48T was a strictly aerobic bacterium, catalase- and oxidase-positive. The strain was able to grow at NaCl concentrations of 0.5–10.0 % (w/v), with optimum growth occurring at 2.5 % (w/v) NaCl. The optimum temperature and pH for growth were 35 °C and pH 7.5–8.0, respectively. On the basis of 16S rRNA gene sequence analysis, strain B48T was shown to belong to the genus Bacillus within the phylum Firmicutes and showed the closest phylogenetic similarity to the species Bacillus foraminis CV53T (97.4 %) and Bacillus purgationiresistens DS22T (96.9 %). The DNA G+C content of this new isolate was 40.1 mol%. The major cellular fatty acids of strain B48T were iso-C15 : 0 and anteiso-C15 : 0, and its polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an aminophospholipid and two unknown phospholipids. The only quinone present was menaquinone 7 (MK-7). The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. All these features confirm the placement of isolate B48T within the genus Bacillus . DNA–DNA hybridization experiments revealed a low level of relatedness between strain B48T and Bacillus foraminis IBRC-M 10625T (8.1 %). On the basis of polyphasic evidence from this study, a new species of the genus Bacillus , Bacillus persicus sp. nov., is proposed, with strain B48T ( = IBRC-M 10115T = DSM 25386T = CECT 8001T) as the type strain.

Halomonas huangheensis sp. nov., a moderately halophilic bacterium isolated from a saline–alkali soil

2014 ◽  
Vol 64 (Pt_3) ◽  
pp. 915-920 ◽  
Author(s):  
Chaohua Miao ◽  
Fangfang Jia ◽  
Yusong Wan ◽  
Wei Zhang ◽  
Min Lin ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Content Type ◽  
Moderately Halophilic ◽  
Link Type ◽  
Alkali Soil ◽  
Moderately Halophilic Bacterium

A novel, Gram-stain-negative, aerobic, rod-shaped, non-motile and moderately halophilic bacterium, designated strain BJGMM-B45T, was isolated from a saline–alkali soil collected from Shandong Province, China. Growth of strain BJGMM-B45T occurred at 10–45 °C (optimum, 30 °C) and pH 5.0–12.0 (optimum, pH 7.0) on Luria–Bertani agar medium with 1–20 % (w/v) NaCl (optimum, 7–10 %). The predominant respiratory quinone was Q-9. The major cellular fatty acids (>5 %) were C18 : 1ω7c, C16 : 0, C19 : 0 cyclo ω8c, summed feature 3, C12 : 0 3-OH and C12 : 0. The genomic DNA G+C content was 57.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BJGMM-B45T belonged to the genus Halomonas in the class Gammaproteobacteria . The closest relatives were Halomonas cupida DSM 4740T (98.2 % 16S rRNA gene sequence similarity) and Halomonas denitrificans M29T (97.8 %). Levels of DNA–DNA relatedness between strain BJGMM-B45T and Halomonas cupida CGMCC 1.2312T and Halomonas denitrificans DSM 18045T were 57.0 and 58.9 %, respectively. On the basis of phenotypic, chemotaxonomic and phylogenetic features, strain BJGMM-B45T is considered to represent a novel species of the genus Halomonas , for which the name Halomonas huangheensis sp. nov. is proposed. The type strain is BJGMM-B45T ( = ACCC 05850T = KCTC 32409T).

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