Halomonas huangheensis sp. nov., a moderately halophilic bacterium isolated from a saline–alkali soil

2014 ◽  
Vol 64 (Pt_3) ◽  
pp. 915-920 ◽  
Author(s):  
Chaohua Miao ◽  
Fangfang Jia ◽  
Yusong Wan ◽  
Wei Zhang ◽  
Min Lin ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Content Type ◽  
Moderately Halophilic ◽  
Link Type ◽  
Alkali Soil ◽  
Moderately Halophilic Bacterium

A novel, Gram-stain-negative, aerobic, rod-shaped, non-motile and moderately halophilic bacterium, designated strain BJGMM-B45T, was isolated from a saline–alkali soil collected from Shandong Province, China. Growth of strain BJGMM-B45T occurred at 10–45 °C (optimum, 30 °C) and pH 5.0–12.0 (optimum, pH 7.0) on Luria–Bertani agar medium with 1–20 % (w/v) NaCl (optimum, 7–10 %). The predominant respiratory quinone was Q-9. The major cellular fatty acids (>5 %) were C18 : 1ω7c, C16 : 0, C19 : 0 cyclo ω8c, summed feature 3, C12 : 0 3-OH and C12 : 0. The genomic DNA G+C content was 57.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BJGMM-B45T belonged to the genus Halomonas in the class Gammaproteobacteria . The closest relatives were Halomonas cupida DSM 4740T (98.2 % 16S rRNA gene sequence similarity) and Halomonas denitrificans M29T (97.8 %). Levels of DNA–DNA relatedness between strain BJGMM-B45T and Halomonas cupida CGMCC 1.2312T and Halomonas denitrificans DSM 18045T were 57.0 and 58.9 %, respectively. On the basis of phenotypic, chemotaxonomic and phylogenetic features, strain BJGMM-B45T is considered to represent a novel species of the genus Halomonas , for which the name Halomonas huangheensis sp. nov. is proposed. The type strain is BJGMM-B45T ( = ACCC 05850T = KCTC 32409T).

scholarly journals Halomonas ramblicola sp. nov., a moderately halophilic bacterium from Rambla Salada, a Mediterranean hypersaline rambla

2012 ◽  
Vol 62 (Pt_12) ◽  
pp. 2903-2909 ◽  
Author(s):  
Rocío Luque ◽  
Victoria Béjar ◽  
Emilia Quesada ◽  
Fernando Martínez-Checa ◽  
Inmaculada Llamas
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Sequence Similarity ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Content Type ◽  
Moderately Halophilic ◽  
Link Type ◽  
Moderately Halophilic Bacterium

A moderately halophilic bacterium (strain RS-16T) was isolated from saline soil in Rambla Salada, a Mediterranean hypersaline rambla in Murcia, south-east Spain. Cells of strain RS-16T were Gram-negative rods, oxidase-negative and motile by peritrichous flagella. Strain RS-16T required NaCl for growth, and grew between 1 % and 30 % (w/v) NaCl (optimum, 5–7.5 %), at temperatures of between 4 °C and 41 °C (optimum, 32–37 °C), and at pH values of between 5 and 10 (optimum, pH 7). Strain RS-16T was chemo-organotrophic and its metabolism was respiratory with oxygen and nitrate as terminal electron acceptors. It produced acids from d-glucose and myo-inositol, accumulated poly-β-hydroxyalkanoate granules and produced cream colonies on MY 7.5 % (w/v). The DNA G+C content of strain RS-16T was 56.2 mol%. A comparison of 16S rRNA gene sequences confirmed the relationship of strain RS-16T to species of the genus Halomonas . The most phylogenetically related species was Halomonas cerina SP4T (97.4 %16S rRNA gene sequence similarity). In DNA–DNA hybridization assays strain RS-16T showed DNA–DNA relatedness values of 62.7±3.09 %, 64.5±1.97 % and 64.7±1.74 % to Halomonas cerina CECT 7282T, Halomonas cerina CECT 7284 and Halomonas cerina CECT 7283, respectively. The major fatty acids of strain RS-16T were C18 : 1ω7c and C16 : 0, and the predominant respiratory lipoquinone was ubiquinone, with nine isoprene units (Q-9). On the basis of these data, strain RS-16T is considered to represent a novel species of the genus Halomonas , for which the name Halomonas ramblicola sp. nov. is proposed. The type strain is RS-16T ( = CECT 7896T = LMG 26647T).

Saliterribacillus persicus gen. nov., sp. nov., a moderately halophilic bacterium isolated from a hypersaline lake

2013 ◽  
Vol 63 (Pt_1) ◽  
pp. 345-351 ◽  
Author(s):  
Mohammad Ali Amoozegar ◽  
Maryam Bagheri ◽  
Maryam Didari ◽  
Seyed Abolhassan Shahzedeh Fazeli ◽  
Peter Schumann ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Halophilic Bacterium ◽  
Hypersaline Lake ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Moderately Halophilic Bacterium ◽  
The Family

A novel Gram-positive, moderately halophilic bacterium, designated strain X4BT, was isolated from soil around the hypersaline lake Aran-Bidgol in Iran and characterized taxonomically using a polyphasic approach. Cells of strain X4BT were motile rods and formed ellipsoidal endospores at a terminal or subterminal position in swollen sporangia. Strain X4BT was a strictly aerobic bacterium, catalase- and oxidase-positive. The strain was able to grow at NaCl concentrations of 0.5–22.5 % (w/v), with optimum growth occurring at 7.5 % (w/v) NaCl. The optimum temperature and pH for growth were 35 °C and pH 7.0. Analysis of 16S rRNA gene sequence revealed that strain X4BT is a member of the family Bacillaceae , constituting a novel phyletic lineage within this family. Highest sequence similarities were obtained with the 16S rRNA gene sequences of the type strains of Sediminibacillus albus (96.0 %), Paraliobacillus ryukyuensis (95.9 %), Paraliobacillus quinghaiensis (95.8 %) and Sediminibacillus halophilus (95.7 %), respectively. The DNA G+C content of this novel isolate was 35.2 mol%. The major cellular fatty acids of strain X4BT were anteiso-C15 : 0 and anteiso-C17 : 0 and its polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, two aminolipids, an aminophospholipid and an unknown phospholipid. The isoprenoid quinones were MK-7 (89 %) and MK-6 (11 %). The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. On the basis of 16S rRNA gene sequence analysis in combination with chemotaxonomic and phenotypic data, strain X4BT represents a novel species in a new genus in the family Bacillaceae , order Bacillales for which the name Saliterribacillus persicus gen. nov., sp. nov. is proposed. The type strain of the type species (Saliterribacillus persicus) is X4BT ( = IBRC-M 10629T = KCTC 13827T).

Marinococcus tarijensis sp. nov., a moderately halophilic bacterium isolated from a salt mine

2013 ◽  
Vol 63 (Pt_9) ◽  
pp. 3319-3323 ◽  
Author(s):  
Andrea Balderrama-Subieta ◽  
Daniel Guzmán ◽  
Hiroaki Minegishi ◽  
Akinobu Echigo ◽  
Yasuhiro Shimane ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Sequence Similarity ◽  
Halophilic Bacterium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Moderately Halophilic Bacterium

A Gram-stain-positive, coccoid-shaped, halophilic bacterium, strain SR-1T, was isolated from a salt crystal obtained from a mine located in Tarija, Bolivia. The strain was investigated using a polyphasic approach. The optimum conditions for growth of strain SR-1T were reached at 5 % (w/v) NaCl, pH 7.6 and 37–40 °C. The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The isoprenoid quinone was MK-7. The major cellular fatty acids of strain SR-1T were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The DNA G+C content of strain SR-1T was 48.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed a close relationship between strain SR-1T and Marinococcus halophilus JCM 2479T (99.7 % 16S rRNA gene sequence similarity), Marinococcus halotolerans KCTC 19045T (99.4 %) and Marinococcus luteus KCTC 13214T (99.8 %). However, strain SR-1T also showed low levels of DNA–DNA relatedness with these reference strains (47, 61 and 58 %, respectively). On the basis of phenotypic differences and DNA–DNA hybridization results, strain SR-1T is considered to represent a novel species of the genus Marinococcus , for which the name Marinococcus tarijensis sp. nov. is proposed. The type strain is SR-1T ( = LMG 26930T = CECT 8130T).

Halobacillus fulvus sp. nov., a moderately halophilic bacterium isolated from shrimp paste (Ka-pi) in Thailand

2021 ◽  
Vol 71 (11) ◽  
Author(s):  
Auttaporn Booncharoen ◽  
Wonnop Visessanguan ◽  
Nattakorn Kuncharoen ◽  
Supalurk Yiamsombut ◽  
Pannita Santiyanont ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Type Species ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Content Type ◽  
Moderately Halophilic ◽  
Link Type ◽  
Moderately Halophilic Bacterium

An aerobic, Gram-stain-positive, endospore-forming, rod-shaped and moderately halophilic strain SKP4-6T, was isolated from shrimp paste (Ka-pi) collected from Samut Sakhon Province, Thailand. Phylogenetic analysis revealed that strain SKP4-6T belonged to the genus Halobacillus and was most closely related to Halobacillus salinus JCM 11546T (98.6 %), Halobacillus locisalis KCTC 3788T (98.6 %) and Halobacillus yeomjeoni KCTC 3957T (98.6 %) based on 16S rRNA gene sequence similarity. The digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain SKP4-6T and its related species were 18.2–19.3 % and 69.84–84.51 %, respectively, which were lower than the threshold recommended for species delineation. The strain grew optimally at 30–40 °C, at pH 7.0 and with 10–15 % (w/v) NaCl. It contained l-Orn–d-Asp in the cell wall peptidoglycan. The DNA G+C content was 44.8 mol%. The major fatty acids were iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The predominant isoprenoid quinone was MK-7. Phosphatidylglycerol and diphosphatidylglycerol were present as major polar lipids. Based on this polyphasic approach, digital DNA–DNA relatedness and ANI values, strain SKP4-6T represents a novel species of the genus Halobacillus , for which the name Halobacillus fulvus sp. nov. is proposed. The type strain is SKP4-6T (=JCM 32624T=TISTR 2595T).

Ornithinibacillus halophilus sp. nov., a moderately halophilic, Gram-stain-positive, endospore-forming bacterium from a hypersaline lake

2013 ◽  
Vol 63 (Pt_3) ◽  
pp. 844-848 ◽  
Author(s):  
Maryam Bagheri ◽  
Mohammad Ali Amoozegar ◽  
Peter Schumann ◽  
Maryam Didari ◽  
Malihe Mehrshad ◽  
...  
Keyword(s):  
Type Species ◽  
Halophilic Bacterium ◽  
Hypersaline Lake ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Gram Stain ◽  
Content Type ◽  
Moderately Halophilic ◽  
Link Type ◽  
Moderately Halophilic Bacterium

A novel Gram-stain-positive, moderately halophilic bacterium, designated strain G8BT, was isolated from water of the hypersaline lake Aran-Bidgol in Iran and characterized taxonomically using a polyphasic approach. Cells of strain G8BT were rod-shaped, motile and produced oval endospores at a terminal position in swollen sporangia. Strain G8BT was strictly aerobic, catalase-positive and oxidase-negative. The strain was able to grow at NaCl concentrations of 0.5–12.5 % (w/v), with optimum growth occurring at 5–7.5 % (w/v) NaCl. The optimum temperature and pH for growth were 35–40 °C and pH 7.5–8.0, respectively. On the basis of 16S rRNA gene sequence analysis, strain G8BT was shown to belong to the genus Ornithinibacillus within the phylum Firmicutes and showed closest phylogenetic similarity with Ornithinibacillus bavariensis WSBC 24001T (97.6 %). The DNA G+C content of strain G8BT was 36.9 mol%. The major cellular fatty acids of strain G8BT were anteiso-C15 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C16 : 0, and its polar lipid pattern consisted of phosphatidylglycerol, diphosphatidylglycerol, four unknown phospholipids and an unknown aminolipid. The isoprenoid quinones were MK-7 (98 %) and MK-8 (2 %). Strain G8BT contained a peptidoglycan of type A4β, l-Orn–d-Asp. All these features confirmed the placement of isolate G8BT within the genus Ornithinibacillus . DNA–DNA hybridization experiments revealed a low level of relatedness (6 %) between strain G8BT and Ornithinibacillus bavariensis DSM 15681T. On the basis of evidence from this study, a novel species of the genus Ornithinibacillus , Ornithinibacillus halophilus sp. nov., is proposed, with strain G8BT ( = IBRC-M 10683T = KCTC 13822T) as the type strain.

scholarly journals Aquisalimonas lutea sp. nov., a moderately halophilic bacterium from a saltern

2015 ◽  
Vol 65 (Pt_4) ◽  
pp. 1354-1359 ◽  
Author(s):  
Carmen Infante-Domínguez ◽  
Cristina Sánchez-Porro ◽  
Antonio Ventosa
Keyword(s):  
Dna Hybridization ◽  
Type Species ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Content Type ◽  
Moderately Halophilic ◽  
Link Type ◽  
Moderately Halophilic Bacterium

A yellow-pigmented, motile, Gram-stain-negative, moderately halophilic and strictly aerobic bacterium, designated BA42AL-1T, was isolated from water of a saltern of Santa Pola, Alicante, Spain. Strain BA42AL-1T grew in media containing 5–20 % (w/v) salts (optimum 7.5 % salts). It grew between pH 6.0 and 9.0 (optimally at pH 7.5) and at 15–45 °C (optimally at 37 °C). Phylogenetic analysis based on the comparison of 16S rRNA gene sequences revealed that strain BA42AL-1T is a member of the genus Aquisalimonas . The closest relatives to this strain were Aquisalimonas halophila YIM 95345T and Aquisalimonas asiatica CG12T with sequence similarities of 99.4 % and 97.0 %, respectively. DNA–DNA hybridization between the novel isolate and Aquisalimonas halophila YIM 95345T revealed a relatedness of 54 %. The major fatty acids of strain BA42AL-1T were C18 : 1ω6c/C18 : 1ω7c, C19 : 0 cyclo ω8c and C16 : 0, and lower contents of C12 : 0 and C18 : 0. The polar lipid pattern of strain BA42AL-1T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine, two glycolipids, a lipid and four unknown phospholipids. The G+C content of the genomic DNA of this strain was 65.0 mol%. Based on the DNA–DNA hybridization, phenotypic, chemotaxonomic and phylogenetic data presented in this study, strain BA42AL-1T is proposed as a novel species of the genus Aquisalimonas , for which the name Aquisalimonas lutea sp. nov. is suggested. The type strain is BA42AL-1T ( = CCM 8472T = CECT 8326T = LMG 27614T).

scholarly journals Halomonas icarae sp. nov., a moderately halophilic bacterium isolated from beach soil in India

2020 ◽  
Author(s):  
Kuppusamy Pandiyan ◽  
Prity Kushwaha ◽  
Samadhan Y. Bagul ◽  
Hillol Chakdar ◽  
Munusamy Madhaiyan ◽  
...  
Keyword(s):  
16S Rrna ◽  
Type Species ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Aerobic Bacterium ◽  
Content Type ◽  
Moderately Halophilic ◽  
Link Type ◽  
Moderately Halophilic Bacterium

A moderately halophilic, Gram-stain-negative, aerobic bacterium, strain D1-1T, belonging to the genus Halomonas , was isolated from soil sampled at Pentha beach, Odisha, India. Phylogenetic trees reconstructed based on 16S rRNA genes and multilocus sequence analysis of gyrB and rpoD genes revealed that strain D1-1T belonged to the genus Halomonas and was most closely related to Halomonas alimentaria YKJ-16T (98.1 %) followed by Halomonas ventosae Al12T (97.5 %), Halomonas sediminicola CPS11T (97.5 %), Halomonas fontilapidosi 5CRT (97.4 %) and Halomonas halodenitrificans DSM 735T (97.2 %) on the basis of 16S rRNA gene sequence similarity. Sequence identities with other species within the genus were lower than 97.0 %. The digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values of 22.4–30 % and 79.5–85.4 % with close relatives of H. halodenitrificans DSM 735T, H . alimentaria YKJ-16T, H. ventosae Al12T and H. fontilapidosi 5CRT were lower than the threshold recommended for species delineation (70 % and 95–96 % for dDDH and ANI, respectively). Further, strain D1-1T formed yellow-coloured colonies; cells were rod-shaped, motile with optimum growth at 30 °C (range, 4–45 °C) and 2–8 % NaCl (w/v; grew up to 24 % NaCl). The major fatty acids were summed feature 8 (C18 : 1  ω7c/C18 : 1  ω6c), summed feature 3 (C16 : 1  ω7c/C16 : 1  ω6c) and C16 : 0 and the main respiratory quinone was ubiquinone Q-9 in line with description of the genus. Based on its chemotaxonomic and phylogenetic characteristics and genome uniqueness, strain D1-1T represents a novel species in the genus Halomonas , for which we propose the name Halomonas icarae sp. nov., within the family Halomonadaceae . The type strain is D1-1T (=JCM 33602T=KACC 21317T=NAIMCC-B-2254T).

scholarly journals Alkalibacillus almallahensis sp. nov., a halophilic bacterium isolated from an inland solar saltern

2014 ◽  
Vol 64 (Pt_6) ◽  
pp. 2066-2071 ◽  
Author(s):  
Azahara Pérez-Davó ◽  
Margarita Aguilera ◽  
Alberto Ramos-Cormenzana ◽  
Mercedes Monteoliva-Sánchez
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Related Species ◽  
Type Species ◽  
Halophilic Bacterium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Content Type ◽  
Link Type

A halophilic, Gram-staining-positive, non-motile, endospore forming rod-shaped bacterial strain, S1LM8T, was isolated from a sediment sample collected from an inland solar saltern located in La Malahá, Granada (Spain). Growth was observed in media containing 7.5–30 % total salts (optimum 15 % total salts), at pH 7–10 (optimum pH 8) and at 15–50 °C (optimum 35–38 °C). The predominant isoprenoid quinone was MK-7. It contained A1γ-type peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid. The major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The G+C content of its genomic DNA was 38.2 mol%. The affiliation of strain S1LM8T with the species of the genus Alkalibacillus was determined by 16S rRNA gene sequence comparison. The most closely related species were Alkalibacillus halophilus YIM 012T with 99.8 % similarity, Alkalibacillus salilacus BH163T with 99.8 % similarity and Alkalibacillus flavidus ISL-17T with 98.1 % similarity between their 16S rRNA gene sequences. However, DNA–DNA relatedness between the novel isolate and the related species of the genus Alkalibacillus was less than 34 %. Based on the phylogenetic, phenotypic and chemotaxonomic features, a novel species, Alkalibacillus almallahensis sp. nov. is proposed. The type strain is S1LM8T ( = CECT 8373T = DSM 27545T).

scholarly journals Fodinicurvata halophila sp. nov., a moderately halophilic bacterium from a marine saltern

2015 ◽  
Vol 65 (Pt_3) ◽  
pp. 766-771 ◽  
Author(s):  
Carmen Infante-Dominguez ◽  
Paul A. Lawson ◽  
Crystal N. Johnson ◽  
Cristina Sánchez-Porro ◽  
Antonio Ventosa
Keyword(s):  
Type Species ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
The Novel ◽  
Content Type ◽  
Moderately Halophilic ◽  
Link Type ◽  
Moderately Halophilic Bacterium ◽  
Lipid Pattern ◽  
Sequence Similarities

A Gram-stain-negative, rod-shaped, facultatively anaerobic, moderately halophilic bacterium, designated strain BA45ALT, was isolated from water of a saltern located in Santa Pola, Alicante, Spain. Cells were motile, and catalase- and oxidase-positive. Strain BA45ALT grew at temperatures in the range 14–45 °C (optimally at 37 °C), at pH 5.0–9.0 (optimally at pH 7.5), and in media containing 5–20 % (w/v) salts [optimally in media containing 10 % (w/v) salts]. Phylogenetic analysis based on the comparison of 16S rRNA gene sequences revealed that strain BA45ALT is a member of the genus Fodinicurvata . The closest relatives to the novel strain were Fodinicurvata fenggangensis YIM D812T and Fodinicurvata sediminis YIM D82T with sequence similarities of 98.2 % and 97.4 %, respectively. DNA–DNA hybridization between the novel isolate and these phylogenetically related species revealed relatedness values of 30 % and 15 %, respectively, with respect to the aforementioned species. The major cellular fatty acids of strain BA45ALT were C18 : 1ω7c, C16 : 0 and iso-C15 : 0. The G+C content of the genomic DNA of strain BA45ALT was 58.0 mol%, and the polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine and a number of unknown phospholipids and lipids. Based on phenotypic, chemotaxonomic and phylogenetic data presented in this study, strain BA45ALT constituted a novel species of the genus Fodinicurvata , for which the name Fodinicurvata halophila sp. nov. is suggested. The type strain is BA45ALT ( = CCM 8504T = CECT 8472T = JCM 19075T = LMG 27945T).

scholarly journals Pseudomonas salegens sp. nov., a halophilic member of the genus Pseudomonas isolated from a wetland

2014 ◽  
Vol 64 (Pt_10) ◽  
pp. 3565-3570 ◽  
Author(s):  
Mohammad Ali Amoozegar ◽  
Azadeh Shahinpei ◽  
Abbas Akhavan Sepahy ◽  
Ali Makhdoumi-Kakhki ◽  
Shima Sadat Seyedmahdi ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Sequence Similarity ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Ph Range ◽  
Slightly Halophilic Bacterium

A novel Gram-stain-negative, aerobic, non-endospore-forming, non-pigmented, rod-shaped, slightly halophilic bacterium, designated GBPy5T, was isolated from aquatic plants of the Gomishan wetland, Iran. Cells of strain GBPy5T were motile. Growth occurred with between 1 and 10 % (w/v) NaCl and the isolate grew optimally with 3 % (w/v) NaCl. The optimum pH and temperature for growth of the strain were pH 8.0 and 30 °C, respectively, while it was able to grow over a pH range of 6.5–9.0 and a temperature range of 4–35 °C. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain GBPy5T is a member of the genus Pseudomonas forming a monophyletic branch. The novel strain exhibited 16S rRNA gene sequence similarity of 95.4 % with type strains of Pseudomonas guariconensis PCAVU11T and Pseudomonas sabulinigri J64T, respectively. The major cellular fatty acids of the isolate were C18 : 1ω7c (37.8 %), C16 : 0 (14.9 %), C16 : 1ω7c (12.9 %), C12 : 0 3-OH (7.1 %) and C12 : 0 (7.0 %). The polar lipid pattern of strain GBPy5T comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and one phospholipid. Ubiquinone 9 (Q-9) was the predominant lipoquinone. The G+C content of the genomic DNA of strain GBPy5T was 59.2 mol%. On the basis of the phenotypic and phylogenetic data, strain GBPY5T represents a novel species of the genus Pseudomonas , for which the name Pseudomonas salegens sp. nov. is proposed. The type strain is GBPy5T ( = IBRC-M 10762T = CECT 8338T).

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