Abstract
A Gram-stain-negative, wheat, rod-shaped, motile, non-spore forming, and aerobic bacterium, designated as strain PIT, was isolated from saline silt samples collected in saltern in Yantai, Shandong, China, and subjected to a polyphasic taxonomic study. The 16S rRNA gene sequence analysis revealed a clear affiliation of strain PIT with the genus Kangiella. Strain PIT showed the closest phylogenetic relationship with Kangiella taiwanensis KT1T with the highest 16S rRNA gene sequence similarity of 98.3%. The genome size of the strain PIT was 2809662 bp consisting of 2653 genes which include 39 tRNAs genes, 2 sRNA genes and 4 rRNA genes. The major cellular fatty acids of strain PIT were identified as iso-C15:0, iso-C11:0, iso-C11:0 3-OH and Summed Feature 9 (iso-C17:1ω9c), and the major respiratory isoprenoid quinone was Q8. The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylmonomethylethanolamine (PME) and phosphatidylinositol (PI). The G+C content of the genomic DNA was 45.8 mol%. The Average Amino Acid identity (AAI) value between strain PIT and Kangiella sediminilitoris was 83.9%. The Average Nucleotide Identity (ANI) values between strain PIT and Kangiella sediminilitoris, Kangiella aquimarina and Kangiella taiwanensis were 76.9%, 71.0% and 74.6%, respectively. The digital DNA-DNA Hybridization (dDDH) values between strain PIT and Kangiella sediminilitoris, Kangiella aquimarina and Kangiella taiwanensis were 19.8%, 19.1% and 18.7%, respectively. The results of 16S rRNA gene phylogeny, phenotypic characterizations, chemotaxonomic characterization and genome features indicate that strain PIT represents a novel specie of the genus Kangiella, for which the name Kangiella shandongensis sp. nov. is proposed. The type strain is PIT (KCTC 82509T = MCCC 1K04352T).