scholarly journals Sphingorhabdus arenilitoris sp. nov., isolated from a coastal sand, and reclassification of Sphingopyxis rigui as Sphingorhabdus rigui comb. nov. and Sphingopyxis wooponensis as Sphingorhabdus wooponensis comb. nov.

2014 ◽  
Vol 64 (Pt_8) ◽  
pp. 2551-2557 ◽  
Author(s):  
Ja-Min Park ◽  
Sooyeon Park ◽  
Yong-Taek Jung ◽  
Hyangmi Kim ◽  
Jung-Sook Lee ◽  
...  

A Gram-stain-negative, strictly aerobic, non-flagellated and rod-shaped bacterial strain, designated GJR-7T, was isolated from coastal sand of the South Sea of South Korea. Strain GJR-7T grew optimally at 30 °C, at pH 7.0–7.5 and without NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain GJR-7T clustered with the type strains of Sphingopyxis wooponensis , Sphingopyxis rigui and Sphingorhabdus planktonica , with which it exhibited 16S rRNA gene sequence similarity values of 96.0–96.3 %. Sequence similarities to the type strains of other recognized species were less than 95.5 %. Strain GJR-7T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c, 11-methyl C18 : 1ω7c and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) or C14 : 0 2-OH. The major polar lipids were sphingoglycolipid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and one unidentified glycolipid. The DNA G+C content of strain GJR-7T was 55.5 mol%. Differential phenotypic properties, together with phylogenetic distinctiveness, revealed that strain GJR-7T is separated from the type strains of Sphingopyxis wooponensis , Sphingopyxis rigui and Sphingorhabdus planktonica . On the basis of the data presented, strain GJR-7T is considered to represent a novel species of the genus Sphingorhabdus , for which the name Sphingorhabdus arenilitoris sp. nov. is proposed. The type strain is GJR-7T ( = KCTC 42051T = CECT 8531T). It is also proposed that Sphingopyxis wooponensis and Sphingopyxis rigui should be reclassified as members of the genus Sphingorhabdus .

2014 ◽  
Vol 64 (Pt_6) ◽  
pp. 1841-1846 ◽  
Author(s):  
Sooyeon Park ◽  
Ji-Min Park ◽  
Chul-Hyung Kang ◽  
Jung-Hoon Yoon

A Gram-stain-negative, non-motile, aerobic, curved-to-spiral-rod-shaped bacterium, designated AH-MY2T, was isolated from a tidal flat on Aphae island in the sea to the south-west of South Korea, and its taxonomic position was investigated using a polyphasic taxonomic approach. Strain AH-MY2T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain AH-MY2T clustered with the type strain of Terasakiella pusilla and that this cluster joined the clade comprising the type strains of species of the genus Thalassospira . Strain AH-MY2T exhibited 16S rRNA gene sequence similarity values of 90.6 % to the type strain of Terasakiella pusilla and of less than 91.0 % to the type strains of other species with validly published names. Strain AH-MY2T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The major polar lipids detected in strain AH-MY2T were phosphatidylglycerol, phosphatidylethanolamine, two unidentified aminolipids and one unidentified glycolipid. The DNA G+C content of strain AH-MY2T was 56.0 mol%. The phylogenetic data and differential chemotaxonomic and other phenotypic properties revealed that strain AH-MY2T represented a novel genus and species within the family Rhodospirillaceae of the class Alphaproteobacteria , for which the name Aestuariispira insulae gen. nov., sp. nov. is proposed. The type strain of Aestuariispira insulae is AH-MY2T ( = KCTC 32577T = CECT 8488T).


Author(s):  
Tristan Barbeyron ◽  
Manon Thiébaud ◽  
Nolwen Le Duff ◽  
Marjolaine Martin ◽  
Erwan Corre ◽  
...  

Four marine bacterial strains were isolated from a thallus of the brown alga Ascophyllum nodosum collected in Roscoff, France. Cells were Gram-stain-negative, strictly aerobic, non-flagellated, gliding, rod-shaped and grew optimally at 25–30 °C, at pH 7–8 and with 2–4 % NaCl. Phylogenetic analyses of their 16S rRNA gene sequences showed that the bacteria were affiliated to the genus Zobellia (family Flavobacteriaceae , phylum Bacteroidetes ). The four strains exhibited 97.8–100 % 16S rRNA gene sequence similarity values among themselves, 97.9–99.1 % to the type strains of Zobellia amurskyensis KMM 3526T and Zobellia laminariae KMM 3676T, and less than 99 % to other species of the genus Zobellia . The DNA G+C content of the four strains ranged from 36.7 to 37.7 mol%. Average nucleotide identity and digital DNA–DNA hybridization calculations between the new strains and other members of the genus Zobellia resulted in values of 76.4–88.9 % and below 38.5 %, respectively. Phenotypic, phylogenetic and genomic analyses showed that the four strains are distinct from species of the genus Zobellia with validly published names. They represent two novel species of the genus Zobellia , for which the names Zobellia roscoffensis sp. nov. and Zobellia nedashkovskayae sp. nov. are proposed with Asnod1-F08T (RCC6906T=KMM 6823T=CIP 111902T) and Asnod2-B07-BT (RCC6908T=KMM 6825T=CIP 111904T), respectively, as the type strains.


Author(s):  
Lei Song ◽  
Hongcan Liu ◽  
Ying Huang ◽  
Xin Dai ◽  
Yuguang Zhou

A Gram-stain-negative, rod-shaped bacterial strain, designated SW123T, was isolated from a deep-sea water sample collected from the Indian Ocean. Strain SW123T was strictly aerobic, catalase- and oxidase-positive. The predominant cellular fatty acids were iso-C15 : 0, iso-C17 : 0 and summed feature 9 (comprising C16 : 0-methyl or iso-C17 : 1 ω9c). Ubiquinone-8 was the sole respiratory quinone. The major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The genomic DNA G+C content was 49.4 mol%. 16S rRNA gene sequence analysis showed that strain SW123T was closely related to Aliidiomarina shirensis AIST (96.7 % sequence similarity), Aliidiomarina iranensis GBPy7T (96.3%), Aliidiomarina haloalkalitolerans AK5T (96.0%) and Aliidiomarina celeris F3105T (95.9%). Phylogenetic trees based on 16S rRNA gene sequences showed that strain SW123T represented a novel member of the genus Aliidiomarina , forming a distinct cluster with A. celeris F3105T. On the basis of phylogenetic inference and phenotypic characteristics, we propose that strain SW123T represents a novel species of the genus Aliidiomarina , with the name Aliidiomarina indica sp. nov. The type strain is SW123T (=CGMCC1.16169T=KCTC 82234T).


2013 ◽  
Vol 63 (Pt_5) ◽  
pp. 1684-1689 ◽  
Author(s):  
Sooyeon Park ◽  
Jung-Hoon Yoon

A Gram-negative, non-spore-forming, non-flagellated, coccoid-, oval- or rod-shaped strain, designated M-M23T, was isolated from seashore sediment at Geoje island, South Korea. Strain M-M23T grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain M-M23T clustered with the type strains of the two species of the genus Hirschia , with which it exhibited 97.6–98.1 % 16S rRNA gene sequence similarity. Sequence similarity with the type strains of other recognized species was <90.8 %. Strain M-M23T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c and C16 : 0 as the major fatty acids. The major polar lipids of strain M-M23T were phosphatidylglycerol and two unidentified lipids. The DNA G+C content of strain M-M23T was 45.4 mol%. DNA–DNA relatedness between the isolate and Hirschia baltica DSM 5838T and Hirschia maritima JCM 14974T was 22±7.2 and 14±5.6 %, respectively. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain M-M23T is separate from the other described members of the genus Hirschia . On the basis of the data presented, strain M-M23T is considered to represent a novel species of the genus Hirschia , for which the name Hirschia litorea sp. nov. is proposed. The type strain is M-M23T ( = KCTC 32081T  = CCUG 62793T). An emended description of the genus Hirschia is also provided.


2013 ◽  
Vol 63 (Pt_2) ◽  
pp. 755-760 ◽  
Author(s):  
Dong-Heon Lee ◽  
Jae Seoun Hur ◽  
Hyung-Yeel Kahng

A strictly aerobic, Gram-stain-negative bacterium, designated strain No.6T, was isolated from a lichen (Cladonia sp.) collected in Geogeum Island, Korea, and its taxonomic status was established by a polyphasic study. Cells of strain No.6T were non-motile, catalase- and oxidase-positive, non-spore-forming rods. Growth was observed at 15–35 °C (optimum, 25–30 °C), at pH 5.0–10.0 (optimum, pH 6.0–8.0) and with 0–3 % NaCl (optimum, 0–2 %). The predominant cellular fatty acids were summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1ω7c, 41.5 %), iso-C15 : 0 (26.7 %) and C16 : 0 (9.6 %), and menaquinone MK-7 was the only respiratory quinone. The G+C content of the genomic DNA of strain No.6T was 36.8 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain No.6T fell within the evolutionary group encompassed by the genus Sphingobacterium . Levels of 16S rRNA gene sequence similarity between the novel strain and the type strains of recognized Sphingobacterium species ranged from 92.1 to 99.1 %, the highest values being with Sphingobacterium siyangense SY1T (99.1 %) and Sphingobacterium multivorum IAM 14316T (98.5 %). DNA–DNA relatedness between strain No.6T and these two type strains were 32.0 and 5.7 %, respectively. The polar lipids found in strain No.6T were phosphatidylethanolamine, two unidentified phospholipids, three unidentified aminophospholipids, one glycolipid and four unidentified lipids. One unidentified sphingolipid was also found. On the basis of phenotypic and genotypic data, strain No.6T represents a novel species of the genus Sphingobacterium , for which the name Sphingobacterium cladoniae sp. nov. is proposed. The type strain is No.6T ( = KCTC 22613T = JCM 16113T). An emended description of Sphingobacterium siyangense is also proposed.


2015 ◽  
Vol 65 (Pt_5) ◽  
pp. 1646-1652 ◽  
Author(s):  
P. Kämpfer ◽  
A. B. Arun ◽  
H.-J. Busse ◽  
Zhen-Li Zhang ◽  
Chiu-Chung Young ◽  
...  

Two Gram-stain-negative, non-endospore-forming, rod-shaped bacteria, strains CC-MHSW-5T and A1392, were isolated from water of coastal hot springs located in Taiwan and China, respectively, and investigated for their taxonomic position. The two strains shared identical 16S rRNA gene sequences, a DNA-DNA hybridization value >80 % and similar genomic DNA G+C contents (64.3 and 64.6 mol%), but showed different genomic fingerprint patterns generated by BOX-PCR and three random amplification polymorphic DNA PCRs. The strains shared highest 16S rRNA gene sequence similarities with the type strains of Chelativorans multitrophicus (96.7 and 96.1 %), Thermovum composti (96.2 and 96.1 %) and Chelativorans oligotrophicus (96.1 and 95.8 %). Phylogenetic trees (based on 16S rRNA and recA gene sequence comparisons) showed a distinct clustering of both strains with the type strains of species of the genus Chelativorans and T. composti Nis3T. The quinone systems of strains CC-MHSW-5T and Nis3T contained ubiquinone Q-10 as the major component. The major polyamine in both strains was sym-homospermidine. Putrescine, spermidine and, for strain CC-MHSW-5T, spermine were found in minor concentrations. Their polar lipid profiles consisted of phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and diphosphatidylglycerol. The fatty acid profile contained major amounts of C18 : 1ω7c and C19 : 0 cyclo ω8c. On the basis of these results, the two strains are considered to represent a novel species of the genus Chelativorans , for which the name Chelativorans intermedius sp. nov. is proposed. The type strain is CC-MHSW-5T ( = CCM 8543T = LMG 28482T = DSM 29391T = CIP 110825T). Based on both genotypic and phenotypic characters, it is proposed that T. composti be reclassified within the genus Chelativorans as Chelativorans composti comb. nov.


2015 ◽  
Vol 65 (Pt_4) ◽  
pp. 1262-1267 ◽  
Author(s):  
Ji-Min Park ◽  
Sooyeon Park ◽  
Sung-Min Won ◽  
Yong-Taek Jung ◽  
Kee-Sun Shin ◽  
...  

A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated and rod-shaped or ovoid bacterial strain, designated BG-MY13T, was isolated from a tidal flat sediment on the South Sea, South Korea. Strain BG-MY13T grew optimally at 30–35 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain BG-MY13T falls within the cluster comprising the type strains of species of the genus Gramella . Strain BG-MY13T exhibited 16S rRNA gene sequ4ence similarity values of 96.9–97.8 % to the type strains of Gramella echinicola , Gramella gaetbulicola , Gramella portivictoriae and Gramella marina and of 94.6–96.5 % to the type strains of other species of the genus Gramella with validly published names. Strain BG-MY13T contained MK-6 as the predominant menaquinone and iso-C15 : 0 and iso-C17 : 0 3-OH as the major fatty acids. The major polar lipids were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain BG-MY13T was 38.9 mol% and DNA–DNA relatedness values with the type strains of G. echinicola , G. gaetbulicola , G. portivictoriae and G. marina were 12–23 %. The differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain BG-MY13T is separated from other species of the genus Gramella . On the basis of the data presented, strain BG-MY13T is considered to represent a novel species of the genus Gramella , for which the name Gramella aestuariivivens sp. nov. is proposed. The type strain is BG-MY13T ( = KCTC 42285T = NBRC 110677T).


2014 ◽  
Vol 64 (Pt_6) ◽  
pp. 1984-1990 ◽  
Author(s):  
Young-Ok Kim ◽  
Sooyeon Park ◽  
Bo-Hye Nam ◽  
Yong-Taek Jung ◽  
Dong-Gyun Kim ◽  
...  

A Gram-stain-negative, non-motile, coccoid, ovoid or rod-shaped bacterial strain, designated RSS3-C1T, was isolated from a golden sea squirt (Halocynthia aurantium) collected from the East Sea, South Korea. Strain RSS3-C1T was found to grow optimally at 20–25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain RSS3-C1T clustered with the type strains of Lutimonas vermicola and Aestuariicola saemankumensis . Strain RSS3-C1T exhibited 98.8 % 16S rRNA gene sequence similarity to each type strain. Strain RSS3-C1T contained MK-6 as the predominant menaquinone and iso-C15 : 0, iso-C17 : 0 3-OH and anteiso-C15 : 0 as the major fatty acids. The major polar lipids of strain RSS3-C1T were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain RSS3-C1T was 39.2 mol%, and DNA–DNA relatedness to the type strains of and was 21±5.3 and 26±7.5 %, respectively. The differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain RSS3-C1T is separated from and . On the basis of the data presented, strain RSS3-C1T is considered to represent a novel species of the genus Lutimonas , for which the name Lutimonas halocynthiae sp. nov. is proposed. The type strain is RSS3-C1T ( = KCTC 32537T = CECT 8444T). In this study, it is also proposed that Aestuariicola saemankumensis should be reclassified as a member of the genus Lutimonas , as Lutimonas saemankumensis comb. nov. (type strain SMK-142T = KCTC 22171T = CCUG 55329T), and the description of the genus Lutimonas is emended.


2020 ◽  
Vol 70 (4) ◽  
pp. 2696-2702 ◽  
Author(s):  
Heeyoung Kang ◽  
Jinkyeong Kang ◽  
Inseong Cha ◽  
Haneul Kim ◽  
Yochan Joung ◽  
...  

Two strains of Gram-stain-positive, strictly aerobic, motile, spore-forming, rod-shaped, moderately halotolerant bacteria, designated as HMF5848T and HME7618, were isolated from salt/brine and subjected to a polyphasic taxonomic investigation. Growth of both yellow-coloured strains occurred in the presence of 1–9 % NaCl (w/v; optimum, 2–3 %), at 15–45 °C (optimum, 37 °C) and pH 6–9 (optimum, pH 7). The major fatty acids were iso-C15 : 0, iso-C16 : 0 and anteiso-C15 : 0. The cell-wall peptidoglycan was meso-diaminopimelic acid. The only respiratory quinone was menaquinone-7. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, four unidentified glycolipids, three unidentified phospholipids and two unidentified polar lipids. DNA G+C content was 37.4 mol%. Phylogenetic trees based on 16S rRNA gene sequences showed that strains HMF5848T and HME7618 clustered with Bacillus luteolus YIM 93174T. Strains HMF5848T showed the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %). The values of in silico DNA–DNA hybridization and average nucleotide identity between strains HMF5848T and B. humi DSM 16318T were 25.8 and 69.7 %, respectively. On the basis of phylogenetic, physiological and chemotaxonomic properties, strain HMF5848T represents a novel species, Bacillus salinus sp. nov. The type strain is HMF5848T (=KCTC 43010T=CECT 9695T).


2014 ◽  
Vol 64 (Pt_11) ◽  
pp. 3810-3815 ◽  
Author(s):  
Sooyeon Park ◽  
Ji-Min Park ◽  
Doo-Sang Park ◽  
Jung-Hoon Yoon

A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated GJSW-31T, was isolated from seawater from the South Sea, South Korea. The novel strain grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees, based on 16S rRNA gene sequences, revealed that strain GJSW-31T clustered with the type strains of species of the genus Litoreibacter . Strain GJSW-31T exhibited 16S rRNA gene sequence similarity values of 95.2–98.5 % to the type strains of species of the genus Litoreibacter and sequence similarities of less than 96.18 % to type strains of the other species with validly published names. Strain GJSW-31T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The major polar lipids of strain GJSW-31T were phosphatidylcholine, phosphatidylglycerol, one unidentified lipid and one unidentified aminolipid. The DNA G+C content of strain GJSW-31T was 62.5 mol% and its DNA–DNA relatedness values with the type strains of Litoreibacter albidus , Litoreibacter janthinus , Litoreibacter meonggei and Litoreibacter ascidiaceicola were 13–23 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain GJSW-31T is separate from other species of the genus Litoreibacter . On the basis of the data presented, strain GJSW-31T is considered to represent a novel species of the genus Litoreibacter , for which the name Litoreibacter ponti sp. nov. is proposed. The type strain is GJSW-31T ( = KCTC 42114T = NBRC 110379T).


Sign in / Sign up

Export Citation Format

Share Document