scholarly journals Actinomadura alba sp. nov., isolated from soil in Yunnan, China

2007 ◽  
Vol 57 (8) ◽  
pp. 1735-1739 ◽  
Author(s):  
Yong-Xia Wang ◽  
Xiao-Yang Zhi ◽  
Hua-Hong Chen ◽  
Yu-Qin Zhang ◽  
Shu-Kun Tang ◽  
...  
Keyword(s):  
Yunnan Province ◽  
Novel Species ◽  
Aerial Mycelium ◽  
Genetic Data ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
The Family ◽  
Evolutionary Radiation ◽  
Actinomycete Strain

A novel actinomycete, strain YIM 45681T, which was isolated from soil in a suburb of Kunming, Yunnan Province, China, was subjected to phenotypic and genotypic characterization. The micro-organism, which produces short spore chains arranged in spirals on the aerial mycelium, was shown to have meso-diaminopimelic acid in the cell wall. The sugars present in whole-cell hydrolysates were ribose, xylose, galactose, madurose and glucose. The predominant menaquinones were MK-9(H4), MK-9(H6) and MK-9(H2). The phospholipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside. The major fatty acids were iso-C16 : 1 H, C17 : 1 ω8c, 10-methyl C17 : 0 and C16 : 0. A comparative analysis of 16S rRNA gene sequences indicated that the organism formed a distinct clade within the evolutionary radiation of the family Thermomonosporaceae, and that it was closely associated with members of the genus Actinomadura. A broad range of phenotypic and genetic data supported the suggestion that this organism represents a novel species of the genus Actinomadura, for which the name Actinomadura alba sp. nov. is proposed, with YIM 45681T (=DSM 45045T =CCTCC AA206005T) as the type strain.

Actinoallomurus acaciae sp. nov., an endophytic actinomycete isolated from Acacia auriculiformis A. Cunn. ex Benth.

2010 ◽  
Vol 60 (3) ◽  
pp. 554-559 ◽  
Author(s):  
Arinthip Thamchaipenet ◽  
Chantra Indananda ◽  
Chakrit Bunyoo ◽  
Kannika Duangmal ◽  
Atsuko Matsumoto ◽  
...  
Keyword(s):  
Novel Species ◽  
Aerial Mycelium ◽  
Diaminopimelic Acid ◽  
Acacia Auriculiformis ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Endophytic Actinomycete ◽  
Phyletic Line ◽  
Actinomycete Strain ◽  
Short Spiral

A novel endophytic actinomycete, strain GMKU 931T, was isolated from the root of a wattle tree, Acacia auriculiformis A. Cunn. ex Benth., collected at Kasetsart University, Bangkok, Thailand. Strain GMKU 931T produced short spiral chains of smooth-surfaced spores on the aerial mycelium. Lysine and meso-diaminopimelic acid were present in the cell-wall peptidoglycan. Whole-cell hydrolysates contained galactose, madurose and mannose. The predominant menaquinones were MK-9(H6) and MK-9(H8). The major fatty acids were iso-C16 : 0 and iso-C16 : 1. The major phospholipids were phosphatidylinositol and phosphatidylglycerol. A phylogenetic analysis based on 16S rRNA gene sequences suggested that strain GMKU 931T forms a distinct phyletic line within the recently proposed genus Actinoallomurus. The significant differences in phenotypic and genotypic data indicate that strain GMKU 931T represents a novel species of the genus Actinoallomurus, for which the name Actinoallomurus acaciae sp. nov. is proposed. The type strain is GMKU 931T (=BCC 28622T =NBRC 104354T =NRRL B-24610T).

scholarly journals Planifilum yunnanense sp. nov., a thermophilic thermoactinomycete isolated from a hot spring

2007 ◽  
Vol 57 (8) ◽  
pp. 1851-1854 ◽  
Author(s):  
Yan-xin Zhang ◽  
Chen Dong ◽  
Shen Biao
Keyword(s):  
Yunnan Province ◽  
Hot Spring ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Properties ◽  
Republic Of China ◽  
Actinomycete Strain

A thermophilic actinomycete, strain LA5T, was isolated from a hot spring in Yunnan province, People's Republic of China. The isolate grew aerobically at temperatures of 50–75 °C. Aerobic mycelia were not observed. Single spores were produced along the substrate hypha. Strain LA5T was characterized chemotaxonomically by having MK-7 as the predominant menaquinone and iso-C17 : 0 (27.7 %) and C16 : 0 (22.4 %) as the major fatty acids. The cell wall contained meso-diaminopimelic acid. The DNA G+C content was 56.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain LA5T belongs to the genus Planifilum. Levels of DNA–DNA relatedness between strain LA5T and Planifilum fimeticola H0165T and Planifilum fulgidum 500275T were 43.6 and 52.7 %, respectively. On the basis of phenotypic properties and phylogeny, strain LA5T represents a novel species of the genus Planifilum, for which we propose the name Planifilum yunnanense sp. nov., with the type strain LA5T (=CCTCC AA206002T=KCTC 13052T).

scholarly journals Actinopolymorpha cephalotaxi sp. nov., a novel actinomycete isolated from rhizosphere soil of the plant Cephalotaxus fortunei

2010 ◽  
Vol 60 (1) ◽  
pp. 51-54 ◽  
Author(s):  
Li-Jie Yuan ◽  
Yu-Qin Zhang ◽  
Li-Yan Yu ◽  
Cheng-Hang Sun ◽  
Yu-Zhen Wei ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Yunnan Province ◽  
Rhizosphere Soil ◽  
Novel Species ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Hybridization Data ◽  
Actinomycete Strain ◽  
Physiological And Biochemical

An actinomycete, strain I06-2230T, was isolated from rhizosphere soil of the plant Cephalotaxus fortunei, collected from Yunnan province, south China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the genus Actinopolymorpha. Cells grew on agar surfaces, with no penetration even after prolonged cultivation. Aerial hyphae were absent. Cells were irregularly shaped and remained attached as chains or aggregates. Chemotaxonomic data, which showed ll-diaminopimelic acid in the cell wall, glucose as the whole-cell sugar, type PI phospholipids and MK-9(H4) as the predominant menaquinone, supported the affiliation of strain I06-2230T to the genus Actinopolymorpha. The major fatty acids were iso-C15 : 0, iso-C16 : 0 and iso-C16 : 1 H. The genomic DNA G+C content was 69.3 mol%. DNA–DNA hybridization data, in combination with chemotaxonomic, physiological and biochemical data, demonstrated that strain I06-2230T should be classified as representing a novel species of the genus Actinopolymorpha. The name Actinopolymorpha cephalotaxi sp. nov. is proposed, with strain I06-2230T (=DSM 45117T=CCM 7466T=KCTC 19293T) as the type strain.

Dactylosporangium siamense sp. nov., isolated from soil

2013 ◽  
Vol 63 (Pt_11) ◽  
pp. 4033-4038 ◽  
Author(s):  
Chitti Thawai ◽  
Chanwit Suriyachadkun
Keyword(s):  
Novel Species ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Mycolic Acids ◽  
Cellular Fatty Acids ◽  
Link Type ◽  
Tropical Forest Soil ◽  
Actinomycete Strain

A novel actinomycete strain, designed MW4-36T, was isolated from tropical forest soil in Nakhon Sawan Province, Thailand. Morphological and chemotaxonomic characteristics of this strain clearly demonstrated that it belongs to the genus Dactylosporangium . The strain formed finger-shaped sporangia on short sporangiophores that emerged directly from substrate hyphae. The cell-wall peptidoglycan contained glutamic acid, glycine, alanine and meso-diaminopimelic acid including 3-hydroxy-meso-diaminopimelic acid; arabinose, glucose, rhamnose, ribose and xylose were found as whole-cell sugars. The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol; no phosphatidylcholine was found. The predominant menaquinones were MK-9(H8) and MK-9(H6). Mycolic acids were not detected. The predominant cellular fatty acids were iso-C15 : 0, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The G+C content of the genomic DNA was 72.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences also indicated that the strain should be classified in the genus Dactylosporangium and showed that the closest relative was Dactylosporangium maewongense JCM 15933T (99.4 % similarity). These taxonomic data revealed that strain MW4-36T could be readily distinguished from its phylogenetically closest relative. On the basis of these phenotypic and genotypic data, strain MW4-36T is considered to represent a novel species, for which the name Dactylosporangium siamense sp. nov. is proposed. The type strain is MW4-36T ( = BCC 34901T = NBRC 106093T).

scholarly journals Nocardiopsis arvandica sp. nov., isolated from sandy soil

2011 ◽  
Vol 61 (5) ◽  
pp. 1189-1194 ◽  
Author(s):  
Javad Hamedi ◽  
Fatemeh Mohammadipanah ◽  
Gabriele Pötter ◽  
Cathrin Spröer ◽  
Peter Schumann ◽  
...  
Keyword(s):  
Sandy Soil ◽  
Novel Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Aerial Mycelium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Hybridization Data ◽  
Actinomycete Strain ◽  
Taxonomic Approach

An actinomycete strain, HM7T, was isolated from sandy soil from the banks of the Arvand River, Khoramshahr, Iran. In a study conducted using a polyphasic taxonomic approach, it was found that this strain represented a novel species in the genus Nocardiopsis. Strain HM7T had long, branched substrate mycelia and the aerial mycelium completely fragmented to long chains of rod-shaped spores. The cell wall of strain HM7T contained meso-diaminopimelic acid, galactose and ribose. The predominant phospholipids were phosphatidylglycerol, phosphatidylethanolamine diphosphatidylglycerol, and phosphatidylcholine. The main menaquinones were MK-10(H2), MK-10(H4), MK-10(H0) and MK-9(H2). Strain HM7T showed the highest degree of 16S rRNA gene sequence similarity with Nocardiopsis sinuspersici UTMC 00102T (99.8 %) and Nocardiopsis quinghaiensis YIM 28A4T (99.3 %), but differed significantly from these type strains based on DNA–DNA hybridization data (37.6 and 38.9 % relatedness, respectively). Physiological, biochemical and phenotypic characteristics allowed strain HM7T to be differentiated from related species. On the basis of results from phenotypic and phylogenetic analyses, strain HM7T is considered to represent a novel species of the genus Nocardiopsis, for which the name Nocardiopsis arvandica sp. nov. is proposed; the type strain of this species is HM7T ( = UTMC 00103T = DSM 45278T = CCUG 58831T).

scholarly journals Micromonospora fluminis sp. nov., isolated from mountain river sediment

2020 ◽  
Vol 70 (12) ◽  
pp. 6428-6436 ◽  
Author(s):  
Miladis Isabel Camacho Pozo ◽  
Anneleen D. Wieme ◽  
Suyén Rodríguez Pérez ◽  
Gabriel Llauradó Maury ◽  
Charlotte Peeters ◽  
...  
Keyword(s):  
16S Rrna ◽  
Type Species ◽  
Novel Species ◽  
Aerial Mycelium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Santiago De Cuba ◽  
Actinomycete Strain

During a bioprospection of bacteria with antimicrobial activity, the actinomycete strain A38T was isolated from a sediment sample of the Carpintero river located in the Gran Piedra Mountains, Santiago de Cuba province (Cuba). This strain was identified as a member of the genus Micromonospora by means of a polyphasic taxonomy study. Strain A38T was an aerobic Gram-positive filamentous bacterium that produced single spores in a well-developed vegetative mycelium. An aerial mycelium was absent. The cell wall contained meso-diaminopimelic acid and the whole-cell sugars were glucose, mannose, ribose and xylose. The major cellular fatty acids were isoC15:0, 10 methyl C17:0, anteiso-C17:0 and iso-C17:0. The predominant menaquinones were MK-10(H4) and MK-10(H6). Phylogenetic analysis of 16S rRNA gene sequences revealed that this strain was closely related to Micromonospora tulbaghiae DSM 45142T (99.5 %), Micromonospora citrea DSM 43903T (99.4 %), Micromonospora marina DSM 45555T (99.4 %), Micromonospora maritima DSM 45782T (99.3 %), Micromonospora sediminicola DSM 45794T (99.3 %), Micromonospora aurantiaca DSM 43813T (99.2 %) and Micromonospora chaiyaphumensis DSM 45246T (99.2 %). The results of OrthoANIu analysis showed the highest similarity to Micromonospora chalcea DSM 43026T (96.4 %). However, the 16S rRNA and gyrB gene sequence-based phylogeny and phenotypic characteristics provided support to distinguish strain A38T as a novel species. On the basis of the results presented here, we propose to classify strain A38T (=LMG 30467T=CECT 30034T) as the type strain of the novel species Micromonospora fluminis sp. nov.

Planobispora siamensis sp. nov., isolated from soil

2013 ◽  
Vol 63 (Pt_7) ◽  
pp. 2649-2654 ◽  
Author(s):  
Wipaporn Ngaemthao ◽  
Chanwit Suriyachadkun ◽  
Suwanee Chunhametha ◽  
Tomohiko Tamura ◽  
Jean-Jacques Sanglier
Keyword(s):  
Novel Species ◽  
Aerial Mycelium ◽  
Diaminopimelic Acid ◽  
Evergreen Forest ◽  
Rrna Gene ◽  
Content Type ◽  
Mycolic Acids ◽  
Link Type ◽  
Gene Sequence Analysis ◽  
Actinomycete Strain

A novel actinomycete strain, A-T 4600T, which developed cylindrical sporangia containing a longitudinal pair of motile spores forming singly or in bundles on short ramifications of the aerial mycelium, was isolated from soil collected from an evergreen forest in Thailand. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained ribose, madurose, mannose and glucose. The predominant menaquinones were MK-9(H2). Mycolic acids were not detected. The diagnostic phospholipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylglycerol, phosphatidylinositol mannoside, diphosphatidylglycerol and aminophosphoglycolipid. The predominant cellular fatty acids were unsaturated C17 : 1, C18 : 1, saturated C16 : 0, and C17 : 0. The G+C content of the DNA was 70.8 mol%. Phenotypic and chemotaxonomic analyses showed that the isolate had the typical characteristics of members of the genus Planobispora . Furthermore, 16S rRNA gene sequence analysis also indicated that this strain belonged to the genus Planobispora but as a putative novel species. DNA–DNA relatedness values that differentiate the isolate from previously described members of the genus Planobispora were significantly below 70 %. Following an evaluation of phenotypic, chemotaxonomic and genotypic studies, it is proposed that the isolate represents a novel species, Planobispora siamensis sp. nov.; the type strain is A-T 4600T ( = BCC 39469T = NBRC 107568T).

Actinomadura meridiana sp. nov., isolated from mountain soil

2012 ◽  
Vol 62 (1) ◽  
pp. 217-222 ◽  
Author(s):  
Soon Dong Lee
Keyword(s):  
Novel Species ◽  
Sequence Similarity ◽  
Aerial Mycelium ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Dna Relatedness ◽  
The Family ◽  
The Media ◽  
Type Strains

A novel actinomycete, designated strain DLS-45T, was isolated from soil from the surface of a rock collected from the peak of Darangshi Oreum (Small Mountain) in Jeju, Republic of Korea. Substrate mycelium was well developed, but aerial mycelium was scant on most of the media tested. Spherical to subspherical spores on the aerial mycelium were in chains that were arranged in hooks and their surfaces were warty. The combination of morphological and chemical features supported the classification of the new isolate in the genus Actinomadura. The neighbour-joining tree based on 16S rRNA gene sequences showed that the strain belonged to the family Thermomonosporaceae and formed a coherent cluster with Actinomadura pelletieri IMSNU 22169T (98.0 % sequence similarity). The other closest relatives were Actinomadura bangladeshensis 3-46-b(3)T (98.2 %) and Actinomadura chokoriensis 3-25-a(11)T (97.9 %). Levels of DNA–DNA relatedness between strain DLS-45T and the type strains of the phylogenetic relatives were less than 17 %. A battery of phenotypic, genotypic and DNA–DNA relatedness data indicated that strain DLS-45T represented a novel species of the genus Actinomadura, for which the name Actinomadura meridiana sp. nov. is proposed. The type strain is strain DLS-45T ( = KCTC 19558T = DSM 45252T).

scholarly journals Actinophytocola oryzae gen. nov., sp. nov., isolated from the roots of Thai glutinous rice plants, a new member of the family Pseudonocardiaceae

2010 ◽  
Vol 60 (5) ◽  
pp. 1141-1146 ◽  
Author(s):  
Chantra Indananda ◽  
Atsuko Matsumoto ◽  
Yuki Inahashi ◽  
Yoko Takahashi ◽  
Kannika Duangmal ◽  
...  
Keyword(s):  
New Genus ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Aerial Mycelium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Rice Plants ◽  
Glutinous Rice ◽  
The Family ◽  
Actinomycete Strain

A novel endophytic actinomycete, strain GMKU 367T, was isolated from roots of Thai glutinous rice plants (Oryza sativa L. ‘RD6’) collected from Pathum Thani Rice Research Center, Pathum Thani province, Thailand. Strain GMKU 367T formed cylindrical spores on aerial mycelium, but sporangium-like structures and fragmentation of substrate mycelium were not observed. The cell-wall amino acids contained meso-diaminopimelic acid, alanine, glutamic acid and acetylated muramic acid. The whole-cell sugars were arabinose, galactose, mannose, rhamnose and ribose. Major fatty acids were iso-C15 : 0, iso-C16 : 0 and C16 : 0. The diagnostic menaquinone was MK-9(H4). The polar phospholipids were phosphatidylethanolamine and hydroxyphosphatidylethanolamine. The G+C content of the genomic DNA was 71.1 mol%. Phylogenetic analyses based on 16S rRNA gene sequence data indicated that strain GMKU 367T differed from members of the family Pseudonocardiaceae. On the basis of the evidence presented in this polyphasic study, it is proposed that strain GMKU 367T represents a novel species in a new genus in the family Pseudonocardiaceae, with the name Actinophytocola oryzae gen. nov., sp. nov.; the type strain of Actinophytocola oryzae is GMKU 367T (=BCC 31372T =NBRC 105245T).

scholarly journals Shimazuella kribbensis gen. nov., sp. nov., a mesophilic representative of the family Thermoactinomycetaceae

2007 ◽  
Vol 57 (11) ◽  
pp. 2660-2664 ◽  
Author(s):  
Dong-Jin Park ◽  
Syed G. Dastager ◽  
Jae-Chan Lee ◽  
Soo-Hwan Yeo ◽  
Jung-Hoon Yoon ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
New Genus ◽  
Aerial Mycelium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
The Novel ◽  
16S Rrna Gene Sequences ◽  
The Family ◽  
Sequence Similarities

A mesophilic strain, designated A 9500T, was isolated from a soil sample collected from Sobaek Mountain, South Korea, and its taxonomic position was investigated by using a polyphasic approach. The novel strain grew well on different ISP media and no diffusible pigments were produced. The optimum temperature for growth was 32 °C. The aerial mycelium was well developed, but not fragmented. The strain was Gram-positive, non-motile and formed endospores on vegetative and aerial hyphae with a spiny surface. Cell walls of strain A 9500T contained meso-diaminopimelic acid as the diagnostic amino acid, but no characteristic sugars or other amino acids were found (chemotype-III). The major menaquinone was MK-9(H4) and the minor menaquinone was MK-10(H4) and they were detected at a ratio of 7 : 3. Phosphatidylethanolamine was the diagnostic phospholipid. The G+C content of the genomic DNA was 39.4 mol%. The major fatty acids were anteiso-C15 : 0 (43.34 %), iso-C16 : 0 (14.23 %) and C16 : 0 (7.90 %), a composition that differed from members of related genera of the family Thermoactinomycetaceae. The novel strain formed a distinct clade in a phylogenetic tree based on 16S rRNA gene sequences. On the basis of a range of phenotypic and genotypic data and on 16S rRNA gene sequence similarities (88.35–90.38 %), it is suggested that strain A 9500T represents a novel species in a new genus with the name Shimazuella kribbensis gen. nov., sp. nov. The type strain of the type species is A 9500T (=KCTC 9933T=DSM 45090T).

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