scholarly journals Polymorphisms of the prion protein gene coding region in born-after-the-reinforced-ban (BARB) bovine spongiform encephalopathy cattle in Great Britain

2007 ◽  
Vol 88 (4) ◽  
pp. 1374-1378 ◽  
Author(s):  
G. C. Saunders ◽  
P. C. Griffiths ◽  
S. Cawthraw ◽  
A. C. Tout ◽  
P. Wiener ◽  
...  
Keyword(s):  
Great Britain ◽  
Bovine Spongiform Encephalopathy ◽  
Prion Protein ◽  
Protein Gene ◽  
Transmissible Spongiform Encephalopathy ◽  
Control Measures ◽  
Spongiform Encephalopathy ◽  
Prion Protein Gene ◽  
Coding Region ◽  
Gene Coding

Polymorphisms of the prion protein gene are associated with differing susceptibilities to transmissible spongiform encephalopathy diseases, as shown for variant Creutzfeldt–Jakob disease in humans and scrapie in sheep, but not yet in cattle. Imposition of control measures in the UK, including a reinforced ruminant feed ban in 1996, has led to a reduction in the incidence of bovine spongiform encephalopathy (BSE). BSE-affected cattle born after 1996 in Great Britain have been termed born-after-the-reinforced-ban (BARB) cases. In this study, the PrP gene coding region from 100 BARB BSE cases and 66 matched healthy-control cattle was sequenced to investigate whether this would reveal a genetic basis to their origin. Polymorphisms identified were not found to be associated with increased susceptibility to BSE in the BARB cases. Analysis of BARB cases grouped either by clinical status or by whether they formed an isolated or clustered case was also undertaken, but differences were not found to be significant.

scholarly journals First Report of the Potential Bovine Spongiform Encephalopathy (BSE)-Related Somatic Mutation E211K of the Prion Protein Gene (PRNP) in Cattle

2020 ◽  
Vol 21 (12) ◽  
pp. 4246 ◽  
Author(s):  
Sae-Young Won ◽  
Yong-Chan Kim ◽  
Byung-Hoon Jeong
Keyword(s):  
Bovine Spongiform Encephalopathy ◽  
Prion Protein ◽  
Somatic Mutation ◽  
Prion Disease ◽  
In Silico ◽  
Somatic Mutations ◽  
Protein Gene ◽  
Prnp Gene ◽  
Spongiform Encephalopathy ◽  
Prion Protein Gene

Bovine spongiform encephalopathy (BSE) is a prion disease characterized by spongiform degeneration and astrocytosis in the brain. Unlike classical BSE, which is caused by prion-disease-contaminated meat and bone meal, the cause of atypical BSE has not been determined. Since previous studies have reported that the somatic mutation in the human prion protein gene (PRNP) has been linked to human prion disease, the somatic mutation of the PRNP gene was presumed to be one cause of prion disease. However, to the best of our knowledge, the somatic mutation of this gene in cattle has not been investigated to date. We investigated somatic mutations in a total of 58 samples, including peripheral blood; brain tissue including the medulla oblongata, cerebellum, cortex, and thalamus; and skin tissue in 20 individuals from each breed using pyrosequencing. In addition, we estimated the deleterious effect of the K211 somatic mutation on bovine prion protein by in silico evaluation tools, including PolyPhen-2 and PANTHER. We found a high rate of K211 somatic mutations of the bovine PRNP gene in the medulla oblongata of three Holsteins (10% ± 4.4%, 28% ± 2%, and 19.55% ± 3.1%). In addition, in silico programs showed that the K211 somatic mutation was damaging. To the best of our knowledge, this study is the first to investigate K211 somatic mutations of the bovine PRNP gene that are associated with potential BSE progression.

Polymorphism of the prion protein gene (PRNP) in Polish cattle affected by classical bovine spongiform encephalopathy

2011 ◽  
Vol 39 (5) ◽  
pp. 5211-5217 ◽  
Author(s):  
Artur Gurgul ◽  
Czarnik Urszula ◽  
Magdalena Larska ◽  
Mirosław P. Polak ◽  
Janusz Strychalski ◽  
...  
Keyword(s):  
Bovine Spongiform Encephalopathy ◽  
Prion Protein ◽  
Protein Gene ◽  
Spongiform Encephalopathy ◽  
Prion Protein Gene ◽  
Classical Bovine Spongiform Encephalopathy

Spontaneous mutations in the prion protein gene causing transmissible spongiform encephalopathy

2002 ◽  
Vol 52 (3) ◽  
pp. 355-359 ◽  
Author(s):  
Ayush Dagvadorj ◽  
Robert B. Petersen ◽  
Hee Suk Lee ◽  
Larisa Cervenakova ◽  
Alexey Shatunov ◽  
...  
Keyword(s):  
Prion Protein ◽  
Protein Gene ◽  
Transmissible Spongiform Encephalopathy ◽  
Spongiform Encephalopathy ◽  
Prion Protein Gene ◽  
Spontaneous Mutations

scholarly journals Erratum to: Polymorphism of the prion protein gene (PRNP) in Polish cattle affected by classical bovine spongiform encephalopathy

2012 ◽  
Vol 39 (4) ◽  
pp. 5045-5045 ◽  
Author(s):  
Artur Gurgul ◽  
Urszula Czarnik ◽  
Magdalena Larska ◽  
Mirosław P. Polak ◽  
Janusz Strychalski ◽  
...  
Keyword(s):  
Bovine Spongiform Encephalopathy ◽  
Prion Protein ◽  
Protein Gene ◽  
Spongiform Encephalopathy ◽  
Prion Protein Gene ◽  
Classical Bovine Spongiform Encephalopathy

Detection of Bovine Spongiform Encephalopathy-Related Prion Protein Gene Promoter Polymorphisms in Local Turkish Cattle

2008 ◽  
Vol 46 (11-12) ◽  
pp. 820-827 ◽  
Author(s):  
Cemal Ün ◽  
Kemal Oztabak ◽  
Nehir Özdemir ◽  
Dawit Tesfaye ◽  
Ahmet Mengi ◽  
...  
Keyword(s):  
Bovine Spongiform Encephalopathy ◽  
Prion Protein ◽  
Protein Gene ◽  
Gene Promoter ◽  
Spongiform Encephalopathy ◽  
Prion Protein Gene ◽  
Promoter Polymorphisms

Prion protein gene (PRNP) polymorphisms in native Chinese cattle

Genome ◽  
2010 ◽  
Vol 53 (2) ◽  
pp. 138-145 ◽  
Author(s):  
Hui Zhao ◽  
Xiao-Yan Wang ◽  
Wei Zou ◽  
Ya-Ping Zhang
Keyword(s):  
Prion Protein ◽  
Protein Gene ◽  
Southern China ◽  
Spongiform Encephalopathy ◽  
Prion Protein Gene ◽  
Coding Region ◽  
Low Frequencies ◽  
Frequency Distributions ◽  
Chinese Cattle ◽  
Significant Difference

Polymorphisms in four regions of the bovine prion protein gene (PRNP) confer susceptibility to bovine spongiform encephalopathy (BSE). These polymorphisms include a 23-bp insertion/deletion (indel) in the promoter region, a 12-bp indel in intron 1, an octapeptide repeat or 24-bp indel in the open reading frame, and a single nucleotide polymorphism (SNP) in the coding region. In this study, we investigated the frequency distributions of genotypes, alleles, and haplotypes at these indel sites in 349 native Chinese cattle and sequence variants in 50 samples. Our results showed that cattle in southern China have low frequencies of the 12-bp deletion allele and the 23-bp deletion / 12-bp deletion haplotype, which have been suggested to be relevant to BSE susceptibility. Interestingly, a significant difference was observed between BSE-affected cattle and healthy Chinese cattle in the 12-bp indel polymorphism. A total of 14 SNPs were discovered in the coding region of PRNP in Chinese cattle. Three of these SNPs were associated with amino acid changes (K3T, P54S, and S154N). The E211K substitution that was recently reported in the US atypical BSE case was not detected in this study.

Additions and corrections: Prion protein gene sequence of Canada's first non-imported case of bovine spongiform encephalopathy (BSE)

Genome ◽  
2004 ◽  
Vol 47 (1) ◽  
pp. 229-229
Author(s):  
Michael B Coulthart ◽  
Rhonda Mogk ◽  
Jason M Rancourt ◽  
Deborah L Godal ◽  
Stefanie Czub
Keyword(s):  
Bovine Spongiform Encephalopathy ◽  
Prion Protein ◽  
Gene Sequence ◽  
Protein Gene ◽  
Spongiform Encephalopathy ◽  
Prion Protein Gene ◽  
Imported Case

A new allelic variant in the bovine prion protein gene (PRNP) coding region

1999 ◽  
Vol 30 (5) ◽  
pp. 386-387 ◽  
Author(s):  
J Schläpfer ◽  
N Saitbekova ◽  
C Gaillard ◽  
G Dolf
Keyword(s):  
Prion Protein ◽  
Protein Gene ◽  
Allelic Variant ◽  
Prion Protein Gene ◽  
Coding Region
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