scholarly journals Enzyme kinetics of CRISPR molecular diagnostics

2021 ◽  
Author(s):  
Ashwin Ramachandran ◽  
Juan G. Santiago
Keyword(s):  
Reaction Time ◽  
Enzyme Kinetics ◽  
Molecular Diagnostics ◽  
Analytical Solutions ◽  
Rapid Testing ◽  
Kinetics Parameters ◽  
Diagnostic Assays ◽  
Degree Of Reaction ◽  
Significant Interest ◽  
Kinetics Of

AbstractCRISPR diagnostic assays have gained significant interest in the last few years. This interest has grown rapidly during the current COVID-19 pandemic where CRISPR diagnostics have been frontline contenders for rapid testing solutions. This surge in CRISPR diagnostics research prompts the following question: What exactly are the achievable limits of detection and associated assay times enabled by the kinetics of Cas12 and Cas13 enzymes? To address this question, we here present a model based on Michaelis-Menten enzyme kinetics theory applied to Cas enzymes. We use the model to develop analytical solutions for reaction kinetics and develop back-of-the­ envelope criteria to validate and check for consistency in reported enzyme kinetics parameters. We applied our analyses to all studies known to us which report Michaelis-Menten-type kinetics data for CRISPR associated enzymes. These studies include all subtypes of Cas12 and Cas13 and orthologs. We found all studies but one clearly violate at least two of our three rules of consistency. We further use our model to explore ranges of reaction time scales and degree of reaction completion for practically relevant target concentrations applicable to CRISPR-diagnostic assays.

Kinetic Aspects of the Interaction of Blood Clotting Enzymes

1968 ◽  
Vol 19 (03/04) ◽  
pp. 364-367 ◽  
Author(s):  
H. C Hemker ◽  
P. W Hemker
Keyword(s):  
Enzyme Kinetics ◽  
Blood Clotting ◽  
Competitive Inhibition ◽  
Competitive Inhibitor ◽  
Kinetics Of

SummaryThe enzyme kinetics of competitive inhibition under conditions prevailing in clotting tests are developed and a method is given to measure relative amounts of a competitive inhibitor by means of the t — D plot.

scholarly journals Modelling Activity and Durability of Electrocatalysts from a Statistical Perspective

2021 ◽  
Author(s):  
Jun Huang
Keyword(s):  
Exponential Decay ◽  
Analytical Solutions ◽  
Electrocatalytic Activity ◽  
Active Sites ◽  
Simple Theory ◽  
Exact Analytical Solutions ◽  
Modelling Activity ◽  
Closing The Gap ◽  
The Mean ◽  
Kinetics Of

<p>We develop a theory to investigate how energetic nonhomogeneity of active sites determines the overall activity of an electrocatalyst and how the evolution of the nonhomogeneity determines the overall durability. The simple theory is amenable to exact analytical solutions and thus fosters an in-depth transparent analysis. It is revealed that nonhomogeneity does not necessarily diminish the electrocatalytic activity; instead, the highest overall activity is obtained with a suitable level of nonhomogeneity that is commensurate with the mean property. The evolution kinetics of nonhomogeneity is described by using the Fokker-Planck theory. Exponential decay of the activity is predicted theoretically and confirmed experimentally. The present work represents a first step toward closing the gap between model and practical electrocatalysts using statistical considerations.</p>

scholarly journals Effect of the MgO/Silica Fume Ratio on the Reaction Process of the MgO–SiO2–H2O System

Materials ◽  
2018 ◽  
Vol 12 (1) ◽  
pp. 80 ◽  
Author(s):  
Zhaoheng Li ◽  
Yudong Xu ◽  
Hao Liu ◽  
Jianwei Zhang ◽  
Jiangxiong Wei ◽  
...  
Keyword(s):  
Reaction Kinetics ◽  
Silica Fume ◽  
Main Product ◽  
Magnesium Silicate ◽  
Reaction Process ◽  
Reaction Products ◽  
Degree Of Reaction ◽  
Reaction Thermodynamics ◽  
Experimental Values ◽  
Kinetics Of

In order to clarify the effect of the MgO–silica fume (SF) ratio on the reaction process of the MgO–SiO2–H2O system, the reaction products and degree of reaction were characterized. Furthermore, the parameters of the reaction thermodynamics were calculated and the reaction kinetics were deduced. The results indicate that a large amount of Mg(OH)2 and small quantities of magnesium silicate hydrate (M–S–H) gels were generated upon dissolution of MgO. However, the M–S–H gels were continuously generated until the SF or Mg(OH)2 was consumed completely. For a MgO dosage less than 50% of the total MgO–SiO2–H2O system, the main product was M–S–H gel, while for a MgO dosage greater than 50%, the main product was Mg(OH)2. The results indicate that M–S–H gels have greater stability than Mg(OH)2, and the final reaction product was prone to be M–S–H gels. Based on the experimental values, an equation is proposed for the reaction kinetics of MgO.

scholarly journals Data on biosynthesis of BPAF glucuronide, enzyme kinetics of BPAF glucuronidation, and molecular modeling

Data in Brief ◽  
2019 ◽  
Vol 22 ◽  
pp. 977-986
Author(s):  
Darja Gramec Skledar ◽  
Jurij Trontelj ◽  
Johanna Troberg ◽  
Tihomir Tomašič ◽  
Anamarija Zega ◽  
...  
Keyword(s):  
Molecular Modeling ◽  
Enzyme Kinetics ◽  
Kinetics Of
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