scholarly journals Genetics behind the biosynthesis of nonulosonic acid containing lipooligosaccharides inCampylobacter coli

2018 ◽  
Author(s):  
Alejandra Kolehmainen ◽  
Mirko Rossi ◽  
Jacek Stupak ◽  
Jianjun Li ◽  
Michel Gilbert ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Foodborne Pathogen ◽  
Campylobacter Coli ◽  
Strong Impact ◽  
Large Set ◽  
Donor And Acceptor ◽  
Nonulosonic Acid ◽  
Bacterial Gastroenteritis ◽  
Acute Polyneuropathy

ABSTRACTCampylobacter jejuniandCampylobacter coliare the most common cause of bacterial gastroenteritis in the world. Ganglioside mimicry byC. jejunilipooligosaccharide (LOS) is the triggering factor of Guillain-Barré syndrome (GBS), an acute polyneuropathy. Sialyltransferases from the glycosyltransferase (GT) family 42 are essential for the expression of ganglioside mimics inC. jejuni. Recently, two novel GT-42 genes,cstIVandcstV, have been identified inC. coli.Despite being present in ∼11% of currently availableC. coligenomes, the biological role ofcstIVandcstVis unknown. In the present study, mutation studies in two strains expressing eithercstIVorcstVwere performed and mass spectrometry was used to investigate differences in the chemical composition of LOS. Attempts were made to identify donor and acceptor molecules usingin vitroactivity tests with recombinant GT-42 enzymes. Here, we show that CstIV and CstV are involved inC. coliLOS biosynthesis. In particular,cstVis associated with LOS sialylation, whilecstIVis linked to the addition of a diacetylated nonulosonic acid residue.IMPORTANCEDespite being a major foodborne pathogen,Campylobacter coliglycobiology has been largely neglected. The genetic makeup of theC. colilipooligosaccharide biosynthesis locus was largely unknown until recently.C. coliharbour a large set of genes associated to lipooligosaccharide biosynthesis, including several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide inCampylobacter jejuni. In the present study,C. coliwas found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids. These findings have a strong impact in understandingC. coliecology, host-pathogen interaction, and pathogenesis.

scholarly journals Genetics behind the Biosynthesis of Nonulosonic Acid-Containing Lipooligosaccharides inCampylobacter coli

2019 ◽  
Vol 201 (8) ◽  
Author(s):  
Alejandra Kolehmainen ◽  
Mirko Rossi ◽  
Jacek Stupak ◽  
Jianjun Li ◽  
Michel Gilbert ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Foodborne Pathogen ◽  
Campylobacter Coli ◽  
Strong Impact ◽  
Large Set ◽  
Content Type ◽  
Donor And Acceptor ◽  
Nonulosonic Acid ◽  
Acute Polyneuropathy

ABSTRACTCampylobacter jejuniandCampylobacter coliare the most common causes of bacterial gastroenteritis in the world. Ganglioside mimicry byC. jejunilipooligosaccharide (LOS) is the triggering factor of Guillain-Barré syndrome (GBS), an acute polyneuropathy. Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics inC. jejuni. Recently, two novel GT-42 genes,cstIVandcstV, have been identified inC. coli. Despite being present in ∼11% of currently availableC. coligenomes, the biological role ofcstIVandcstVis unknown. In the present investigation, mutation studies with two strains expressing eithercstIVorcstVwere performed and mass spectrometry was used to investigate differences in the chemical composition of LOS. Attempts were made to identify donor and acceptor molecules usingin vitroactivity tests with recombinant GT-42 enzymes. Here we show that CstIV and CstV are involved inC. coliLOS biosynthesis. In particular,cstVis associated with LOS sialylation, whilecstIVis linked to the addition of a diacetylated nonulosonic acid residue.IMPORTANCEDespite the fact thatCampylobacter colia major foodborne pathogen, its glycobiology has been largely neglected. The genetic makeup of theC. colilipooligosaccharide biosynthesis locus was largely unknown until recently.C. coliharbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide inCampylobacter jejuni. In the present study,C. coliwas found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids. These findings have a strong impact on our understanding ofC. coliecology, host-pathogen interaction, and pathogenesis.

Reduction of Campylobacter jejuni and Campylobacter coli in Poultry Skin by Fruit Extracts

2010 ◽  
Vol 73 (3) ◽  
pp. 477-482 ◽  
Author(s):  
DIANA VALTIERRA-RODRÍGUEZ ◽  
NORMA L. HEREDIA ◽  
SANTOS GARCÍA ◽  
EDUARDO SÁNCHEZ
Keyword(s):  
Antimicrobial Activity ◽  
Campylobacter Jejuni ◽  
Antimicrobial Agents ◽  
Orange Peel ◽  
Campylobacter Coli ◽  
Sour Orange ◽  
Bacterial Gastroenteritis ◽  
Natural Foods ◽  
Promising Source

Campylobacter spp. are a major cause of foodborne bacterial gastroenteritis in humans, and current methods to control Campylobacter contamination in foods are not completely successful. Plants are a promising source of antimicrobial agents, particularly given the growing interest in “all natural” foods. In this study, the antimicrobial activity of extracts from 28 edible plants against Campylobacter jejuni and Campylobacter coli was evaluated in vitro and in a poultry skin model. Nine of 28 extracts exhibited antimicrobial activity in a diffusion assay, and MBCs were determined for the three most active extracts, i.e., lime, plum, and sour orange peel (MBCs of 2 to 3 mg/ml). Mixtures of the lime, plum, and sour orange peel extracts were applied to chicken skin inoculated with 105 CFU of Campylobacter to test for synergistic or antagonist effects. After incubation (48 h at 4°C) with any extract mixture, no Campylobacter CFUs were detectable. A panel of tasters determined that the mixture of lime and plum gave the best flavor to chicken wings. These active extracts from edible fruits are simple to prepare and are alternatives to reduce or eliminate Campylobacter contamination of chicken products.

scholarly journals Activities of Telithromycin, Erythromycin, Fluoroquinolones, and Doxycycline against Campylobacter Strains Isolated from Finnish Subjects

2006 ◽  
Vol 50 (3) ◽  
pp. 1086-1088 ◽  
Author(s):  
Daniela Schönberg-Norio ◽  
Marja-Liisa Hänninen ◽  
Marja-Leena Katila ◽  
Suvi-Sirkku Kaukoranta ◽  
Markku Koskela ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Campylobacter Coli ◽  
Foreign Origin

ABSTRACT The in vitro susceptibilities of 478 Campylobacter jejuni and Campylobacter coli strains isolated from Finnish subjects during 2002 to 2004 were determined. Susceptibility to erythromycin remained high, and telithromycin did not offer any advantage over erythromycin. Reduced susceptibilities to fluoroquinolones and doxycycline were detected almost exclusively among isolates of foreign origin.

scholarly journals A Campylobacter jejuni znuA Orthologue Is Essential for Growth in Low-Zinc Environments and Chick Colonization

2008 ◽  
Vol 191 (5) ◽  
pp. 1631-1640 ◽  
Author(s):  
Lindsay M. Davis ◽  
Tsutomu Kakuda ◽  
Victor J. DiRita
Keyword(s):  
Campylobacter Jejuni ◽  
Transport System ◽  
The United States ◽  
Gram Negative Bacteria ◽  
Limited Growth ◽  
E Coli ◽  
Poultry Products ◽  
Abc Transport ◽  
Bacterial Gastroenteritis

ABSTRACT Campylobacter jejuni infection is a leading cause of bacterial gastroenteritis in the United States and is acquired primarily through the ingestion of contaminated poultry products. Here, we describe the C. jejuni orthologue of ZnuA in other gram-negative bacteria. ZnuA (Cj0143c) is the periplasmic component of a putative zinc ABC transport system and is encoded on a zinc-dependent operon with Cj0142c and Cj0141c, which encode the other two likely components of the transport system of C. jejuni. Transcription of these genes is zinc dependent. A mutant lacking Cj0143c is growth deficient in zinc-limiting media, as well as in the chick gastrointestinal tract. The protein is glycosylated at asparagine 28, but this modification is dispensable for zinc-limited growth and chick colonization. Affinity-purified FLAG-tagged Cj0143c binds zinc in vitro. Based on our findings and on its homology to E. coli ZnuA, we conclude that Cj0143c encodes the C. jejuni orthologue of ZnuA.

scholarly journals Antimicrobial Susceptibilities of Campylobacter Strains Isolated from Finnish Subjects Infected Domestically or from Those Infected Abroad

2003 ◽  
Vol 47 (1) ◽  
pp. 102-105 ◽  
Author(s):  
Hilpi Rautelin ◽  
Antti Vierikko ◽  
Marja-Liisa Hänninen ◽  
Martti Vaara
Keyword(s):  
Campylobacter Jejuni ◽  
Antimicrobial Agents ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Campylobacter Coli ◽  
Antimicrobial Susceptibilities ◽  
Agar Dilution ◽  
Stool Samples

ABSTRACT The in vitro susceptibilities of 678 Campylobacter jejuni and Campylobacter coli strains isolated from stool samples of the same number of Finnish subjects were studied. A total of 523 patients, representing inhabitants from throughout Finland, had not traveled abroad within the 2 weeks prior to becoming ill, whereas 155 persons had presumably acquired their infections abroad. The antimicrobial agents studied were erythromycin, ciprofloxacin, levofloxacin, trovafloxacin, and moxifloxacin. The MICs of these antimicrobial agents were determined by the agar dilution method. The growth of all domestic isolates was inhibited by erythromycin at concentrations of 4 μg/ml, and for these isolates the fluoroquinolone MICs at which 90% of isolates are inhibited (MIC90s) ranged from 0.06 to 0.5 μg/ml. For the foreign isolates, the erythromycin MIC90 was still low (4 μg/ml), but their susceptibilities to fluoroquinolones were clearly reduced (MIC90s, 8 to 64 μg/ml). Of the four different fluoroquinolones studied, ciprofloxacin was the least active (MIC90, 64 μg/ml).

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