Fast Super-Resolution Fluorescence Microscopy Imaging with Low Signal-to-Noise Ratio Based on Deep Learning

2020 ◽  
Vol 47 (10) ◽  
pp. 1007002
Author(s):  
肖康 Xiao Kang ◽  
田立君 Tian Lijun ◽  
王中阳 Wang Zhongyang
2020 ◽  
Vol 25 (2) ◽  
pp. 86-97
Author(s):  
Sandy Suryo Prayogo ◽  
Tubagus Maulana Kusuma

DVB merupakan standar transmisi televisi digital yang paling banyak digunakan saat ini. Unsur terpenting dari suatu proses transmisi adalah kualitas gambar dari video yang diterima setelah melalui proses transimisi tersebut. Banyak faktor yang dapat mempengaruhi kualitas dari suatu gambar, salah satunya adalah struktur frame dari video. Pada tulisan ini dilakukan pengujian sensitifitas video MPEG-4 berdasarkan struktur frame pada transmisi DVB-T. Pengujian dilakukan menggunakan simulasi matlab dan simulink. Digunakan juga ffmpeg untuk menyediakan format dan pengaturan video akan disimulasikan. Variabel yang diubah dari video adalah bitrate dan juga group-of-pictures (GOP), sedangkan variabel yang diubah dari transmisi DVB-T adalah signal-to-noise-ratio (SNR) pada kanal AWGN di antara pengirim (Tx) dan penerima (Rx). Hasil yang diperoleh dari percobaan berupa kualitas rata-rata gambar pada video yang diukur menggunakan metode pengukuran structural-similarity-index (SSIM). Dilakukan juga pengukuran terhadap jumlah bit-error-rate BER pada bitstream DVB-T. Percobaan yang dilakukan dapat menunjukkan seberapa besar sensitifitas bitrate dan GOP dari video pada transmisi DVB-T dengan kesimpulan semakin besar bitrate maka akan semakin buruk nilai kualitas gambarnya, dan semakin kecil nilai GOP maka akan semakin baik nilai kualitasnya. Penilitian diharapkan dapat dikembangkan menggunakan deep learning untuk memperoleh frame struktur yang tepat di kondisi-kondisi tertentu dalam proses transmisi televisi digital.


2020 ◽  
Author(s):  
Hao Li ◽  
DeLiang Wang ◽  
Xueliang Zhang ◽  
Guanglai Gao

Electronics ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 1139 ◽  
Author(s):  
Kai Yang ◽  
Zhitao Huang ◽  
Xiang Wang ◽  
Fenghua Wang

Signal-to-noise ratio (SNR) is a priori information necessary for many signal processing algorithms or techniques. However, there are many problems exsisting in conventional SNR estimation techniques, such as limited application range of modulation types, narrow effective estimation range of signal-to-noise ratio, and poor ability to accommodate non-zero timing offsets and frequency offsets. In this paper, an SNR estimation technique based on deep learning (DL) is proposed, which is a non-data-aid (NDA) technique. Second and forth moment (M2M4) estimator is used as a benchmark, and experimental results show that the performance and robustness of the proposed method are better, and the applied ranges of modulation types is wider. At the same time, the proposed method is not only applicable to the baseband signal and the incoherent signal, but can also estimate the SNR of the intermediate frequency signal.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Elena Cerutti ◽  
Morgana D’Amico ◽  
Isotta Cainero ◽  
Gaetano Ivan Dellino ◽  
Mario Faretta ◽  
...  

AbstractQuantifying the imaging performances in an unbiased way is of outmost importance in super-resolution microscopy. Here, we describe an algorithm based on image correlation spectroscopy (ICS) that can be used to assess the quality of super-resolution images. The algorithm is based on the calculation of an autocorrelation function and provides three different parameters: the width of the autocorrelation function, related to the spatial resolution; the brightness, related to the image contrast; the relative noise variance, related to the signal-to-noise ratio of the image. We use this algorithm to evaluate the quality of stimulated emission depletion (STED) images of DNA replication foci in U937 cells acquired under different imaging conditions. Increasing the STED depletion power improves the resolution but may reduce the image contrast. Increasing the number of line averages improves the signal-to-noise ratio but facilitates the onset of photobleaching and subsequent reduction of the image contrast. Finally, we evaluate the performances of two different separation of photons by lifetime tuning (SPLIT) approaches: the method of tunable STED depletion power and the commercially available Leica Tau-STED. We find that SPLIT provides an efficient way to improve the resolution and contrast in STED microscopy.


Sensors ◽  
2019 ◽  
Vol 19 (10) ◽  
pp. 2270 ◽  
Author(s):  
Kai Yang ◽  
Zhitao Huang ◽  
Xiang Wang ◽  
Xueqiong Li

Spectrum sensing is one of the technologies that is used to solve the current problem of low utilization of spectrum resources. However, when the signal-to-noise ratio is low, current spectrum sensing methods cannot well-handle a situation in which the prior information of the licensed user signal is lacking. In this paper, a blind spectrum sensing method based on deep learning is proposed that uses three kinds of neural networks together, namely convolutional neural networks, long short-term memory, and fully connected neural networks. Experiments show that the proposed method has better performance than an energy detector, especially when the signal-to-noise ratio is low. At the same time, this paper also analyzes the effect of different long short-term memory layers on detection performance, and explores why the deep-learning-based detector can achieve better performance.


Sensors ◽  
2019 ◽  
Vol 19 (19) ◽  
pp. 4076
Author(s):  
Yang ◽  
Zhu ◽  
Wang ◽  
Yang ◽  
Wu ◽  
...  

Raman spectroscopy visualization is a challenging task due to the interference of complex background noise and the number of selected measurement points. In this paper, a super-resolution image reconstruction algorithm for Raman spectroscopy is studied to convert raw Raman data into pseudo-color super-resolution imaging. Firstly, the Raman spectrum data of a single measurement point is measured multiple times to calculate the mean value to remove the random background noise, and innovatively introduce the Retinex algorithm and the median filtering algorithm which improve the signal-to-noise ratio. The novel method of using deep neural network performs a super-resolution reconstruction operation on the gray image. An adaptive guided filter that automatically adjusts the filter radius and penalty factor is proposed to highlight the contour of the cell, and the super-resolution reconstruction of the pseudo-color image of the Raman spectrum is realized. The average signal-to-noise ratio of the reconstructed pseudo-color image sub-band reaches 14.29 db, and the average value of information entropy reaches 4.30 db. The results show that the Raman-based cell pseudo-color image super-resolution reconstruction algorithm is an effective tool to effectively remove noise and high-resolution visualization. The contrast experiments show that the pseudo-color image Kullback–Leiber (KL) entropy of the color image obtained by the method is small, the boundary is obvious, and the noise is small, which provide technical support for the development of sophisticated single-cell imaging Raman spectroscopy instruments.


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