scholarly journals Human Mitochondrial DNA Replication

2012 ◽  
Vol 4 (12) ◽  
pp. a012971-a012971 ◽  
Author(s):  
I. J. Holt ◽  
A. Reyes
Keyword(s):  
Mitochondrial Dna ◽  
Dna Replication ◽  
Human Mitochondrial Dna ◽  
Mitochondrial Dna Replication

scholarly journals A rapid method to visualize human mitochondrial DNA replication through rotary shadowing and transmission electron microscopy

2021 ◽  
Author(s):  
Martin Kosar ◽  
Daniele Piccini ◽  
Marco Foiani ◽  
Michele Giannattasio
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Mitochondrial Dna ◽  
Dna Replication ◽  
Positive Staining ◽  
Human Mitochondrial Dna ◽  
Transmission Electron ◽  
Mitochondrial Dna Replication ◽  
Rotary Shadowing

Abstract We report a rapid experimental procedure based on high-density in vivo psoralen inter-strand DNA cross-linking coupled to spreading of naked purified DNA, positive staining, low-angle rotary shadowing, and transmission electron microscopy (TEM) that allows quick visualization of the dynamic of heavy strand (HS) and light strand (LS) human mitochondrial DNA replication. Replication maps built on linearized mitochondrial genomes and optimized rotary shadowing conditions enable clear visualization of the progression of the mitochondrial DNA synthesis and visualization of replication intermediates carrying long single-strand DNA stretches. One variant of this technique, called denaturing spreading, allowed the inspection of the fine chromatin structure of the mitochondrial genome and was applied to visualize the in vivo three-strand DNA structure of the human mitochondrial D-loop intermediate with unprecedented clarity.

Selective inhibition of mutant human mitochondrial DNA replication in vitro by peptide nucleic acids

1997 ◽  
Vol 15 (2) ◽  
pp. 212-215 ◽  
Author(s):  
Robert W. Taylor ◽  
Patrick F. Chinnery ◽  
Douglass M. Turnbull ◽  
Robert N. Lightowlers
Keyword(s):  
Mitochondrial Dna ◽  
Dna Replication ◽  
Nucleic Acids ◽  
Peptide Nucleic Acids ◽  
Selective Inhibition ◽  
Human Mitochondrial Dna ◽  
Mitochondrial Dna Replication
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