High-Resolution Fluorescent In Situ Hybridization of Drosophila Embryos and Tissues

2008 ◽  
Vol 2008 (6) ◽  
pp. pdb.prot5019-pdb.prot5019 ◽  
Author(s):  
E. Lecuyer ◽  
A. S. Necakov ◽  
L. Caceres ◽  
H. M. Krause
1994 ◽  
Vol 65 (1-2) ◽  
pp. 130-135 ◽  
Author(s):  
J. Inazawa ◽  
T. Ariyama ◽  
T. Tokino ◽  
A. Tanigami ◽  
Y. Nakamura ◽  
...  

Cytometry ◽  
2001 ◽  
Vol 45 (1) ◽  
pp. 1-12 ◽  
Author(s):  
M. Kozubek ◽  
S. Kozubek ◽  
E. Luk�?ov� ◽  
E. B�rtov� ◽  
M. Skaln�kov� ◽  
...  

Author(s):  
Gary Bassell ◽  
Robert H. Singer

We have been investigating the spatial distribution of nucleic acids intracellularly using in situ hybridization. The use of non-isotopic nucleotide analogs incorporated into the DNA probe allows the detection of the probe at its site of hybridization within the cell. This approach therefore is compatible with the high resolution available by electron microscopy. Biotinated or digoxigenated probe can be detected by antibodies conjugated to colloidal gold. Because mRNA serves as a template for the probe fragments, the colloidal gold particles are detected as arrays which allow it to be unequivocally distinguished from background.


2007 ◽  
Vol 177 (4S) ◽  
pp. 596-597
Author(s):  
Joseph P. Alukal ◽  
Bobby B. Najari ◽  
Wilson Chuang ◽  
Lata Murthy ◽  
Monica Lopez-Perdomo ◽  
...  

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