Transformation-defective, Temperature-sensitive Mutants of Rous Sarcoma Virus Have a Reversibly Defective src-gene Product

1980 ◽  
Vol 44 (0) ◽  
pp. 1007-1012 ◽  
Author(s):  
R. R. Friis ◽  
B. M. Jockusch ◽  
C. B. Boschek ◽  
A. Ziemiecki ◽  
H. Rubsamen ◽  
...  
Keyword(s):  
Gene Product ◽  
Rous Sarcoma Virus ◽  
Temperature Sensitive ◽  
Temperature Sensitive Mutants ◽  
Rous Sarcoma ◽  
Src Gene ◽  
Sarcoma Virus

Functional domains of the pp60v-src protein as revealed by analysis of temperature-sensitive Rous sarcoma virus mutants

1984 ◽  
Vol 4 (8) ◽  
pp. 1508-1514
Author(s):  
A W Stoker ◽  
P J Enrietto ◽  
J A Wyke
Keyword(s):  
Rous Sarcoma Virus ◽  
Kinase Activity ◽  
Temperature Sensitive ◽  
Tyrosine Kinase Activity ◽  
Rous Sarcoma ◽  
Heat Labile ◽  
Src Gene ◽  
Sarcoma Virus

Four temperature-sensitive (ts) Rous sarcoma virus src gene mutants with lesions in different parts of the gene represent three classes of alteration in pp60src. These classes are composed of mutants with (i) heat-labile protein kinase activities both in vitro and in vivo (tsLA27 and tsLA29), (ii) heat-labile kinases in vivo but not in vitro (tsLA33), and (iii) neither in vivo nor in vitro heat-labile kinases (tsLA32). The latter class indicates the existence of structural or functional pp60src domains that are required for transformation but do not grossly affect tyrosine kinase activity.

Avian sarcoma viruses, protein kinases and cell transformation

1983 ◽  
Vol 302 (1108) ◽  
pp. 151-156
Keyword(s):  
Cell Transformation ◽  
Temperature Sensitive ◽  
Permissive Temperature ◽  
Cells In Culture ◽  
Temperature Sensitive Mutants ◽  
Rous Sarcoma ◽  
Src Gene ◽  
Sarcoma Virus ◽  
Avian Sarcoma ◽  
Transforming Gene

The first RNA tumour virus to be isolated and identified as such was the Rous sarcoma virus (RSV), which causes the transformation of cells in culture as well as fibrosarcomas when injected into suitable host animals (for reviews see Hanafusa (1977) and Bishop (1978)). The genome of RSV has been studied intensively for the past 10-12 years, and it has been shown that the virus itself carries a gene responsible for malignant transformation. This gene, denoted src for sarcoma, was identified genetically through the isolation of temperature-sensitive mutants that were conditional for cell transformation in culture. These mutants are able to transform cells at a temperature of 35 °C, the permissive temperature, but are unable to transform cells morphologically at 41 °C, the non-permissive temperature. The existence of such temperature sensitive mutants implied that the product of the viral transforming gene, in RSV, was a protein (Kawai & Hanafusa 1971). In addition to temperature-sensitive mutants, non-conditional mutants were isolated that had deletions of the src gene. These mutants are unable to transform cells in culture or to cause fibrosarcomas under most conditions. About 4 years ago, the product of the src gene was identified as a phosphoprotein ( M t = 60000); this protein was denoted pp60 src (Purchio et al. 1978). The RSV genome and the expression of the src gene is illustrated in figure 1.

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