scholarly journals Differentiation of Quail Myoblasts Transformed with a Temperature Sensitive Mutant of Rous Sarcoma Virus. I. Relationship between Differentiation and Tyrosine Kinase of src Gene Product.

1992 ◽  
Vol 17 (4) ◽  
pp. 237-247 ◽  
Author(s):  
Jeman Kim ◽  
Toshiyuki Adachi ◽  
Etsuko Hirayama ◽  
Tadaaki Yabubayashi ◽  
Yoshio Okada
Keyword(s):  
Tyrosine Kinase ◽  
Gene Product ◽  
Rous Sarcoma Virus ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Rous Sarcoma ◽  
Src Gene ◽  
Sarcoma Virus

Aggregation properties of chondrocytes infected with a temperature-sensitive mutant of Rous sarcoma virus

1980 ◽  
Vol 43 (1) ◽  
pp. 407-417
Author(s):  
A. Tanaka ◽  
A. Kaji
Keyword(s):  
Normal Level ◽  
Rous Sarcoma Virus ◽  
Cell Aggregation ◽  
Temperature Sensitive ◽  
Permissive Temperature ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Rous Sarcoma ◽  
Sarcoma Virus ◽  
Level Of Aggregation

Aggregation capacity of chicken embryo chondrocytes decreases when transformed by Rous sarcoma viruses. Cell-to-cell aggregation capacity of chondrocytes infected with a T class temperature-sensitive mutant (tsNY68) (with the temperature-sensitive lesion at the src gene) of Rous sarcoma virus is dependent upon the temperature at which these cells are grown. When grown at the permissive temperature (36 degrees C), where the transforming gene is expressed, aggregation capacity was lower than normal while infected cells grown at the non-permissive temperature (41.5 degrees C) had similar capacity to aggregate to that of normal chondrocytes. However, after a prolonged period of culture (10 days), chondrocytes transformed by wild type SR-RSV regained the normal level of aggregation capacity. Cells transformed by tsNY68 and incubated at the permissive temperature for 10 days also regained the normal level of aggregation capacity. It appears therefore that RSV-transformed chondrocytes first become less adhesive but during long-term cultivation they regain their property to aggregate. The decrease of aggregation capacity due to T class mutants of RSV at 36 degrees C is dependent on constant maintenance of protein synthesis because addition of cycloheximide restored the aggregation capacity even at the permissive temperature.

Transformation of chicken embryo retinal melanoblasts by a temperature-sensitive mutant of rous sarcoma virus

Cell ◽  
1977 ◽  
Vol 11 (4) ◽  
pp. 881-890 ◽  
Author(s):  
Kate Roby ◽  
John Brumbaugh ◽  
Judy Biehl ◽  
Howard Holtzer ◽  
David Boettiger
Keyword(s):  
Rous Sarcoma Virus ◽  
Chicken Embryo ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Rous Sarcoma ◽  
Sarcoma Virus

Transformation-defective, Temperature-sensitive Mutants of Rous Sarcoma Virus Have a Reversibly Defective src-gene Product

1980 ◽  
Vol 44 (0) ◽  
pp. 1007-1012 ◽  
Author(s):  
R. R. Friis ◽  
B. M. Jockusch ◽  
C. B. Boschek ◽  
A. Ziemiecki ◽  
H. Rubsamen ◽  
...  
Keyword(s):  
Gene Product ◽  
Rous Sarcoma Virus ◽  
Temperature Sensitive ◽  
Temperature Sensitive Mutants ◽  
Rous Sarcoma ◽  
Src Gene ◽  
Sarcoma Virus
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