Three-Dimensional Structure from Single Two-Dimensional Diffraction Intensity Measurement

The head to tail connector of bacteriophages plays a fundamental role in the assembly of viral heads and DNA packaging. In spite of the absence of sequence homology, the structure of connectors from different viruses (T4, Ø29, T3, P22, etc) share common morphological features, that are most clearly revealed in their three-dimensional structure. We have studied the three-dimensional reconstruction of the connector protein from phage T3 (gp 8) from tilted view of two dimensional crystals obtained from this protein after cloning and purification.DNA sequences including gene 8 from phage T3 were cloned, into Bam Hl-Eco Rl sites down stream of lambda promotor PL, in the expression vector pNT45 under the control of cI857. E R204 (pNT89) cells were incubated at 42°C for 2h, harvested and resuspended in 20 mM Tris HC1 (pH 7.4), 7mM 2 mercaptoethanol, ImM EDTA. The cells were lysed by freezing and thawing in the presence of lysozyme (lmg/ml) and ligthly sonicated. The low speed supernatant was precipitated by ammonium sulfate (60% saturated) and dissolved in the original buffer to be subjected to gel nitration through Sepharose 6B, followed by phosphocellulose colum (Pll) and DEAE cellulose colum (DE52). Purified gp8 appeared at 0.3M NaCl and formed crystals when its concentration increased above 1.5 mg/ml.

Download Full-text

Three-dimensional structure of bovine heart NADH: ubiquinone oxidoreductase (complex I) by electron microscopy of a single negatively stained two-dimensional crystal

Microscopy ◽

10.1093/jmicro/dft082 ◽

2014 ◽

Vol 63 (2) ◽

pp. 167-174 ◽

Cited By ~ 9

Author(s):

Satoru Shimada ◽

Kyoko Shinzawa-Itoh ◽

Satoko Amano ◽

Yui Akira ◽

Atsuo Miyazawa ◽

...

Keyword(s):

Electron Microscopy ◽

Complex I ◽

Three Dimensional ◽

Dimensional Structure ◽

Two Dimensional ◽

Three Dimensional Structure ◽

Ubiquinone Oxidoreductase ◽

Bovine Heart ◽

Nadh Ubiquinone Oxidoreductase ◽

Dimensional Crystal

Download Full-text

[7] Three-dimensional structure of membrane proteins determined by two-dimensional crystallization, electron cryomicroscopy, and image analysis

Methods in Enzymology - Ion Channels Part C ◽

10.1016/s0076-6879(99)94010-7 ◽

1999 ◽

pp. 135-180 ◽

Cited By ~ 32

Author(s):

Mark Yeager ◽

Vinzenz M. Unger ◽

Alok K. Mitra

Keyword(s):

Image Analysis ◽

Membrane Proteins ◽

Three Dimensional ◽

Dimensional Structure ◽

Two Dimensional ◽

Electron Cryomicroscopy ◽

Three Dimensional Structure

Download Full-text

Three-dimensional structure determination by electron microscopy of two-dimensional crystals

Progress in Biophysics and Molecular Biology ◽

10.1016/0079-6107(83)90017-2 ◽

1982 ◽

Vol 39 ◽

pp. 183-231 ◽

Cited By ~ 403

Author(s):

L.A. Amos ◽

Richard Henderson ◽

P.N.T. Unwin

Keyword(s):

Electron Microscopy ◽

Structure Determination ◽

Three Dimensional ◽

Dimensional Structure ◽

Two Dimensional ◽

Three Dimensional Structure

Download Full-text

Assembled structures of tetrakis(biimidazole)dirhodium complexes hydrogen-bonded with common inorganic anions

Acta Crystallographica Section B Structural Science Crystal Engineering and Materials ◽

10.1107/s2052520614021428 ◽

2014 ◽

Vol 70 (6) ◽

pp. 1006-1019 ◽

Cited By ~ 1

Author(s):

Jin-Long ◽

Kazuhiro Uemura ◽

Masahiro Ebihara

Keyword(s):

Hydrogen Bonding ◽

Three Dimensional ◽

Inorganic Anions ◽

Dimensional Structure ◽

Two Dimensional ◽

Discrete Structure ◽

Torsion Angles ◽

One Dimensional ◽

Three Dimensional Structure ◽

Dirhodium Complexes

Eight new structures of dirhodium complexes, each with four biimidazole (H2bim) ligands, were obtained: [Rh2(H2bim)4(H2O)2](NO3)4·4H2O (I), [Rh2(H2bim)4(H2O)2](ClO4)4·5H2O (II), [Rh2(H2bim)4(MeOH)2](ClO4)4(III), [Rh2(H2bim)4(DMF)2](BF4)4(IV), [Rh2(H2bim)4(Mepy)2](SiF6)2·8H2O (V), [{Rh2(H2bim)4(pz)}2(μ-pz)](SiF6)(ClO4)6·12.7H2O (VI), [{Rh2(H2bim)4(pz)}2(μ-pz)](ClO4)8·11.4H2O (VII) and [Rh2(H2bim)4(μ-pz)](SiF6)2·6H2O (VIII). The unbridged Rh—Rh bond distances range between 2.6313 (8) and 2.7052 (5) Å. The dirhodium units adopt a staggered conformation with torsion angles N—Rh—Rh—N of 37.6 (4)–48.98 (8)°. Various assembled structures were constructed by hydrogen bonding between the complexes and the anions: a discrete structure in (IV), one-dimensional structure in (II), two-dimensional structures in (I), (III), (VI), (VII) and (VIII) and a three-dimensional structure in (V).

Download Full-text