scholarly journals A Putative Protein O-Fucosyltransferase Facilitates Pollen Tube Penetration through the Stigma–Style Interface

2018 ◽  
Vol 176 (4) ◽  
pp. 2804-2818 ◽  
Author(s):  
Devin K. Smith ◽  
Danielle M. Jones ◽  
Jonathan B. R. Lau ◽  
Edward R. Cruz ◽  
Elizabeth Brown ◽  
...  



HortScience ◽  
1991 ◽  
Vol 26 (5) ◽  
pp. 585-586 ◽  
Author(s):  
Sierd Zijlstra ◽  
Coen Purimahua ◽  
Pim Lindhout

Crossing barriers between white- and purple-flowered species were examined. Four accessions of Capsicum annuum and three of C. pubescens were reciprocally crossed with one to four accessions of C. baccatum, C. cardenasii, C. chacoense, C. chinense, C. eximium, C. frutescens, C. galapagoense, and C. praetermissum. Capsicum chacoense is the only white-flowered species that inhibits C. annuum pollen tube growth but allows C. pubescens pollen tube penetration into the egg cell. Capsicum cardenasii and C. eximium exhibit similar crossabilities with C. annuum and C. pubescens: pollen tubes of C. cardenasii and of C. eximium can penetrate the egg cells of C. annuum but not vice versa, and pollen tubes of C. pubescens can penetrate the egg cells of C. cardenasii and of C. eximium but not vice versa.



HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 852E-852
Author(s):  
Dan Ga'ash ◽  
Israel David ◽  
Malka Cohen

Blossom thinning trials with AKZO Co. surfactant Armothin were carried out on fruitful peach cultivars Early Grande and Babcock during 1993–94. Effective thinning occurred before “full bloom” (40% to 90% FB) at 3% Armothin, increasing between 2% and 4%. However, an improved fruit distribution of `Early Grande' was achieved by repeated application (35% + 75% FB) at 2%. A second spray at 3%, just after FB, thinned some late-blooming flowers on `Babcock' trees, but a temporary leach scorch occurred, as well as with 4% Armothin (single spray) on both cultivars. For a single spray, the optimal stage was found within 60% to 90% FB, at 3% Armothin. Flower biology studies showed susceptibility of the petals to increasing Armothin concentrations at all stages, but pollen tube penetration into the pistils and subsequent fertilization failed only after an earlier application, before anthesis or pollination of the stigma. Within this range of concentration and timing, no damage occurred to the vital fruit set and to commercial yield, provided that weather conditions were favorable during bloom (and spray). Some corrective hand-thinning (20% to 60%) should be applied to the fruitful trees 3 to 4 weeks later to achieve optimal fruit size at harvest. Blossom hand-thinning is still practical in Israel.





1995 ◽  
Vol 82 (2) ◽  
pp. 186-196 ◽  
Author(s):  
Juliette Janson ◽  
Michiel T. M. Willemse




2002 ◽  
Vol 15 (2) ◽  
pp. 65-74 ◽  
Author(s):  
Simon Hiscock ◽  
Dominic Bown ◽  
Sarah Gurr ◽  
Hugh Dickinson


1981 ◽  
Vol 30 (1-2) ◽  
pp. 101-122 ◽  
Author(s):  
H. J. Wilms


Zygote ◽  
1993 ◽  
Vol 1 (2) ◽  
pp. 143-154 ◽  
Author(s):  
Bing-Quan Huang ◽  
Elisabeth S. Pierson ◽  
Scott D. Russell ◽  
Antonio Tiezzi ◽  
Mauro Cresti

The cytoskeletal organisation of the isolated embryo sac and egg cells of Plumbago zeylanica was examined before, during and after pollen tube penetration into the embryo sac to determine the potential involvement of microtubules and actin filaments in fertilisation. Material was singly and triply stained using Hoechst 33258 to localise DNA, fluorescein isothiocyanate (FITC)-labelled anti- α-tubulin to detect microtubules and rhodamine-phalloidin to visualise F-actin. Microtubules in the unfertilised egg cell are longitudinally aligned in the micropylar and mid-lateral areas, aggregating into bundles near the filiform apparatus. In the perinuclear cytoplasm of the egg cell, microtubules become more or less randomly aligned. F-actin bundles form a longitudinally aligned mesh in the chalazal cytoplasm of the egg cell. In the central cell, microtubules and F-actin are distributed along transvacuolar strands and are also evident in the perinuclear region and at the periphery of the cell. During pollen tube penetration, sparse microtubule bundles near the pathway of the pollen tube may form an apparent microtubular ‘conduit’ surrounding the male gametes at the delivery site. Actin aggregates become organised near the pathway of the pollen tube and at the delivery site of the sperm cells. Subsequently, actin aggregates form a ‘corona’ structure in the intercellular region between the egg and central cell where gametic fusion occurs. The corona may have a role in maintaining the close proximity of the egg and central cell and helping the two sperm cells move and bind to their target cells. The cytoskeleton may also be involved in causing the two nuclei of the egg and central cell to approach one another at the site of gametic fusion and transporting the two sperm nuclei into alignment with their respective female nucleus. The cytoskeleton is reorganised during early embryogenesis.



Plants ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 185 ◽  
Author(s):  
Valentin Joly ◽  
Faïza Tebbji ◽  
André Nantel ◽  
Daniel P. Matton

Sexual reproduction in flowering plants involves intimate contact and continuous interactions between the growing pollen tube and the female reproductive structures. These interactions can trigger responses in distal regions of the flower well ahead of fertilization. While pollination-induced petal senescence has been studied extensively, less is known about how pollination is perceived at a distance in the ovary, and how specific this response is to various pollen genotypes. To address this question, we performed a global transcriptomic analysis in the ovary of a wild potato species, Solanum chacoense, at various time points following compatible, incompatible, and heterospecific pollinations. In all cases, pollen tube penetration in the stigma was initially perceived as a wounding aggression. Then, as the pollen tubes grew in the style, a growing number of genes became specific to each pollen genotype. Functional classification analyses revealed sharp differences in the response to compatible and heterospecific pollinations. For instance, the former induced reactive oxygen species (ROS)-related genes while the latter affected genes associated to ethylene signaling. In contrast, incompatible pollination remained more akin to a wound response. Our analysis reveals that every pollination type produces a specific molecular signature generating diversified and specific responses at a distance in the ovary in preparation for fertilization.



Sign in / Sign up

Export Citation Format

Share Document