scholarly journals Studies on Nitrogen-Fixing Blue-Green Algae. I. Growth and Nitrogen Fixation by Anabaena Cylindrica Lemm

1955 ◽  
Vol 30 (4) ◽  
pp. 366-372 ◽  
Author(s):  
M. B. Allen ◽  
Daniel I. Arnon
1942 ◽  
Vol 19 (1) ◽  
pp. 78-87
Author(s):  
G. E. FOGG

1. Anabaena cylindrica Lemin. has been obtained in pure unialgal bacteria-free culture. 2. Due precautions having been taken against contamination by other organisms and error due to absorption of fixed nitrogen from the atmosphere, this alga has been shown to possess the capacity of fixing nitrogen. 3. Nitrogen fixation does not take place in the presence of a sufficient quantity of readily available combined nitrogen.


1970 ◽  
Vol 37 (1) ◽  
pp. 49-53
Author(s):  
MA Gafur ◽  
Soltana Parvin

Occurrence of blue-green algae in plain land, pond side, hilly area and dry rice field soils of Chittagong University Campus and N2 fixation of some of them have been studied. Twenty four species under 20 genera were identified and their number varied from 0.11×104/g to 2.8×104/g soil. Anabaena oryzae, Calothrix sp., Cylindrospermum majus and Hapalosiphon hibernicus and Nostoc spongiaeforme were selected for their nitrogen fixing potential. Maximum amount of total nitrogen was recorded in Nostoc spongiaeforme followed by Hapalosiphon hibernicus, Cylindrospermum majus, Anabaena oryzae and Calothrix sp. after 30 days growth in batch cultures.   Key words: BGA, Distribution, Nitrogen fixation doi:10.3329/bjb.v37i1.1563 Bangladesh J. Bot. 37(1): 49-53, 2008 (June)


1969 ◽  
Vol 172 (1029) ◽  
pp. 357-366 ◽  

When carbon dioxide fixation was over 90 % inhibited by CMU , nitrogen fixation remained unaffected in nitrogen-starved cells of Anabaena cylindrica . In normal cells under the same conditions nitrogen fixation was about 50 % inhibited by CMU . These data suggest, first, that nitrogen fixation in this organism is independent of reducing potential generated by non-cyclic photo-electron transport and, secondly, that nitrogen fixation is stimulated by photosynthetically produced carbon skeletons to assimilate the fixed nitrogen. Although nitrogen fixation occurred to a limited extent in the dark, increasing light intensity stimulated nitrogen fixation both in the presence and absence of CMU . This suggests that light-generated ATP is required for nitrogen fixation in this alga. A ratio of pyruvate decarboxylation to nitrogen fixation of 3:1 has been established for A. cylindrica . This accords with the hypothesis that pyruvate acts as a hydrogen donor for nitrogen reduction and that provision of the required reductant is independent of photosynthesis in blue-green algae.


The International Biological Programme served as a focal point for studies on biological nitrogen fixation during the 1960s. The introduction of the acetylene reduction technique for measuring nitrogenase activity in the field led to estimates becoming available of the contribution of lichens, blue-green algae, nodulated non-legumes and bacterial-grass associations, as well as of legumes. Other studies carried out on the physiology and biochemistry of the process led to the eventual purification and characterization of the nitrogenase enzyme. These studies, collectively, provided the springboard for current work, so essential in view of the present energy crisis, on how to increase the use and efficiency of nitrogen-fixing plants, on the metabolic regulation of the nitrogenase enzyme and on the genetics of the nitrogen-fixing process, both in higher plants and in free-living micro-organisms.


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