scholarly journals Metabolism of Inositol(1,4,5)trisphosphate by a Soluble Enzyme Fraction from Pea (Pisum sativum) Roots

1991 ◽  
Vol 95 (2) ◽  
pp. 412-419 ◽  
Author(s):  
B. K. Drøbak ◽  
P. A. C. Watkins ◽  
J. A. Chattaway ◽  
K. Roberts ◽  
A. P. Dawson
Keyword(s):  
Pisum Sativum ◽  
Soluble Enzyme ◽  
Enzyme Fraction ◽  
Inositol 1,4,5 Trisphosphate

scholarly journals Biochemical and cytochemical studies on enzymes that dephosphorylate inositol (1,4,5)-trisphosphate in neutrophils.

1991 ◽  
Vol 39 (3) ◽  
pp. 321-329 ◽  
Author(s):  
J A Badwey ◽  
J M Robinson
Keyword(s):  
Soluble Enzyme ◽  
Alkaline Ph ◽  
Ph Optimum ◽  
Cell Stimulation ◽  
Permeabilized Cells ◽  
Inositol 1,4,5 Trisphosphate ◽  
Biochemical Studies ◽  
Cytochemical Reaction ◽  
Membrane Bound ◽  
C 50

Guinea pig neutrophils contain membrane-bound and soluble phosphatases that catalyze the dephosphorylation of inositol (1,4,5)-trisphosphate [Ins(1,4,5)P3]. The activities were 5.1 +/- 0.2 and 1.3 +/- 0.2 (SD; n = 5) nmoles phosphate (Pi) released/min/10(7) cell equivalents, respectively. The membrane-bound enzyme dephosphorylated many substrates (e.g., beta-glycerophosphate), exhibited alkaline pH optima, and was inhibited by levamisole. In contrast, the soluble phosphatase was specific for Ins(1,4,5)P3, exhibited a neutral pH optimum, and was insensitive to levamisole. A cerium-based ultrastructural cytochemical procedure was employed to identify the subcellular sites of the membrane-bound activity. Staining was observed on the exterior of the plasmalemma and in a population of granules. Staining in the granules was observed only in permeabilized cells. Treatment of neutrophils with p-diazobenzenesulfonate (DBSA) (4.0 mM) for 20 min at 37 degrees C blocked the cytochemical reaction on the cell surface using beta-glycerophosphate as the substrate, but did not affect the staining of the granules on subsequent permeabilization. In biochemical studies, this treatment with DBSA inhibited the membrane-bound activity by c. 50% but did not affect the soluble phosphatase. Therefore, the membrane-bound phosphatase is, in fact, an alkaline phosphatase that resides in locales not accessible to Ins(1,4,5)P3 generated during cell stimulation. Breakdown of Ins(1,4,5)P3 generated during cell stimulation, therefore, would be catalyzed by the soluble enzyme.

Gene expression during two alternative pathways of ovary development in Pisum sativum: fruit development and ovary senescence

1992 ◽  
Vol 85 (1) ◽  
pp. 69-76 ◽  
Author(s):  
Maria-Jose Sanchez-Beltran ◽  
Juan Carbonell ◽  
Jose L. Garcia-Martinez ◽  
Isabel Lopez-Diaz
Keyword(s):  
Gene Expression ◽  
Pisum Sativum ◽  
Fruit Development ◽  
Ovary Development ◽  
Alternative Pathways ◽  
Ovary Senescence

Mitigation Effects of 24-Epibrassinolide and Thiourea in Field Pea (Pisum sativum L.) under Drought Stress

2018 ◽  
Vol 34 (2) ◽  
pp. 229-235 ◽  
Author(s):  
Prachi Garg ◽  
◽  
A. Hemantaranjan ◽  
Jyostnarani Pradhan ◽  
◽  
...  
Keyword(s):  
Drought Stress ◽  
Pisum Sativum ◽  
Field Pea ◽  
Pisum Sativum L

PISUM SATIVUM (LINN): TOXICITY TEST OF m-XYLOHYDROQUINONE WHEN USED AS AN ORAL CONTRACEPTIVE

1956 ◽  
Vol 23 (4_Suppl) ◽  
pp. S93-S97 ◽  
Author(s):  
S. N. Sanyal ◽  
S. C. Banerjee ◽  
Jyoti Sen
Keyword(s):  
Oral Contraceptive ◽  
Pisum Sativum ◽  
Toxicity Test
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