High-pressure protein crystallography (HPPX): instrumentation, methodology and results on lysozyme crystals

A new pneumatic diamond anvil cell has been constructed, generating continuous pressure and temperature variations in the range 0–2.5 GPa and 293–393 K. The cell is designed mainly for high-pressure macromolecular crystallography and should facilitate pressure and temperature annealing of the sample. The analysis is reported of several diffraction data sets of tetragonal hen egg-white lysozyme crystals loaded either in the new cell or in a currently used membrane-based cell. These experiments were performed on beamline FIP-BM30A at the ESRF, Grenoble, a macromolecular crystallography beamline on a bending magnet. Cells were handled and automatically centred by a six-axis robotic arm that was used as a goniometer for data collection by the oscillation method.

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An interferometric study of the solubility of lysozyme crystals under high pressure

Journal of Crystal Growth ◽

10.1016/s0022-0248(99)00746-0 ◽

2000 ◽

Vol 209 (4) ◽

pp. 1018-1022 ◽

Cited By ~ 18

Author(s):

Yoshihisa Suzuki ◽

Tsutomu Sawada ◽

Satoru Miyashita ◽

Hiroshi Komatsu ◽

Gen Sazaki ◽

...

Keyword(s):

High Pressure ◽

Interferometric Study ◽

Lysozyme Crystals

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High-Pressure Protein Crystallography and NMR to Explore Protein Conformations

Annual Review of Biophysics ◽

10.1146/annurev-biophys-042910-155304 ◽

2011 ◽

Vol 40 (1) ◽

pp. 81-98 ◽

Cited By ~ 38

Author(s):

Marcus D. Collins ◽

Chae Un Kim ◽

Sol M. Gruner

Keyword(s):

High Pressure ◽

Protein Crystallography ◽

Protein Conformations

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Protein crystallography beamline BL2S1 at the Aichi synchrotron

Journal of Synchrotron Radiation ◽

10.1107/s1600577516018579 ◽

2017 ◽

Vol 24 (1) ◽

pp. 338-343 ◽

Cited By ~ 21

Author(s):

Nobuhisa Watanabe ◽

Takayuki Nagae ◽

Yusuke Yamada ◽

Ayana Tomita ◽

Naohiro Matsugaki ◽

...

Keyword(s):

High Pressure ◽

Diamond Anvil Cell ◽

Protein Crystallography ◽

X Ray Diffraction ◽

Open Flow ◽

X Ray ◽

Industrial Organizations ◽

Ccd Detector ◽

Diamond Anvil ◽

Cutoff Filter

The protein crystallography beamline BL2S1, constructed at one of the 5 T superconducting bending-magnet ports of the Aichi synchrotron, is available to users associated with academic and industrial organizations. The beamline is mainly intended for use in X-ray diffraction measurements of single-crystals of macromolecules such as proteins and nucleic acids. Diffraction measurements for crystals of other materials are also possible, such as inorganic and organic compounds. BL2S1 covers the energy range 7–17 keV (1.8–0.7 Å) with an asymmetric-cut curved single-crystal monochromator [Ge(111) or Ge(220)], and a platinum-coated Si mirror is used for vertical focusing and as a higher-order cutoff filter. The beamline is equipped with a single-axis goniometer, a CCD detector, and an open-flow cryogenic sample cooler. High-pressure protein crystallography with a diamond anvil cell can also be performed using this beamline.

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