This article studies experimentally the damage behaviors of a 59.5-m-long composite wind turbine blade under accelerated fatigue loads using acoustic emission technique. First, the spectral analysis using the fast Fourier transform is used to study the components of acoustic emission signals. Then, three important objectives including the attenuation behaviors of acoustic emission waves, the arrangement of sensors as well as the detection and positioning of defect sources in the composite blade by developing the time-difference method among different acoustic emission sensors are successfully reached. Furthermore, the clustering analysis using the bisecting K-means method is performed to identify different damage modes for acoustic emission signal sources. This work provides a theoretical and technique support for safety precaution and maintaining of in-service blades.