Fourier Ptychographic Microscopy Reconstruction Method Based on Weight Optimization

Author(s):  
Xiaoli Wang ◽  
Yan Piao ◽  
Yuanshang Jin ◽  
Jie Li ◽  
Qiuyue Han ◽  
...  
Author(s):  
Neng-Yu Zhang ◽  
Terence Wagenknecht ◽  
Michael Radermacher ◽  
Tom Obrig ◽  
Joachim Frank

We have reconstructed the 40S ribosomal subunit at a resolution of 4 nm using the single-exposure pseudo-conical reconstruction method of Radermacher et al.Small (40S) ribosomal subunits were Isolated from rabbit reticulocytes, applied to grids and negatively stained (0.5% uranyl acetate) in a manner that “sandwiches” the specimen between two layers of carbon. Regions of the grid exhibiting uniform and thick staining were identified and photographed twice (magnification 49,000X). The first micrograph was always taken with the specimen tilted by 50° and the second was of the Identical area untilted (Fig. 1). For each of the micrographs the specimen was subjected to an electron dose of 2000-3000 el/nm2.Three hundred thirty particles appearing in the L view (defined in [4]) were selected from both tilted- and untilted-specimen micrographs. The untilted particles were aligned and their rotational alignment produced the azimuthal angles of the tilted particles in the conical tilt series.


2009 ◽  
Vol 129 (1) ◽  
pp. 15-21 ◽  
Author(s):  
Shoichi Urano ◽  
Takeshi Yamada ◽  
Yoshifumi Ooura ◽  
Youheng Xu ◽  
Yasutaka Yamaguchi ◽  
...  

2017 ◽  
Vol E100.B (3) ◽  
pp. 417-425 ◽  
Author(s):  
Stephane KAPTCHOUANG ◽  
Hiroki TAHARA ◽  
Eiji OKI

2020 ◽  
Vol 25 (3) ◽  
pp. 265-276
Author(s):  
K.M. Shepilova ◽  
◽  
A.V. Sotnikov ◽  
A.V. Shipatov ◽  
Yu.V. Savchenko ◽  
...  

2020 ◽  
Author(s):  
Christopher Michael Jones ◽  
◽  
Yngve B Johansen ◽  
Artur Kotwicki ◽  
Cameron Rekully ◽  
...  

2020 ◽  
Author(s):  
James Doorley ◽  
Kristina Volgenau ◽  
Kerry Kelso ◽  
Todd Barrett Kashdan ◽  
Alexander J. Shackman

Background:Retrospective studies have found that people with elevated social anxiety (SA) show a preference for digital/online communication, which may be due to perceptions of enhanced emotional safety. Whether these preferences for/benefits of digital compared to face-to-face communication manifest in the real world has yet to be explored. Methods: We recruited samples of college students (N = 125) and community adults (N = 303) with varying levels of SA, sampled their emotions during digital and face-to-face communication using ecological momentary assessment (EMA) (Study 1) and a day reconstruction method (DRM) (Study 2), and preregistered our hypotheses (https://osf.io/e4y7x/). Results: Results from both studies showed that SA did not predict the likelihood of engaging in digital compared to face-to-face communication, and SA was associated with less positive and more negative emotions regardless of communication medium. Study 2 also showed that whether digital communication was synchronous (e.g., in real time via phone/video chat) or asynchronous (e.g., texting/instant messaging) did not impact the association between SA and emotions. Limitations: EMA and DRM methods, despite their many advantages, may be suboptimal for assessing the occurrence of digital communication behaviors relative to more objective methods (e.g., passively collecting smartphone communication data). Using event-contingent responding may have also yielded more reports of digital communication, thus strengthening our power to detect small, cross-level interaction effects. Conclusions:These results challenge beliefs that digital/online communication provides a source of emotional safety for people with elevated SA and suggests a greater need to address SA-related emotional impairments across digital communication platforms.


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