Cloning, Characterization and Application of the Promoter Region of the Alkaline Protease Gene in Bacillus alcalophillus PB92

2009 ◽  
Author(s):  
Kun Chen ◽  
Yong Jiang ◽  
Nan Wang ◽  
Xuegang Luo ◽  
Fuping Lu ◽  
...  
Keyword(s):  
Alkaline Protease ◽  
Promoter Region ◽  
Protease Gene ◽  
Alkaline Protease Gene

Using 16S rDNA as Target Site for Homologous Recombination to Improve the Alkaline Protease Production of Bacillus alcalophilus

2014 ◽  
Vol 886 ◽  
pp. 349-354
Author(s):  
Qing Shan Mo ◽  
Yao Tian ◽  
Hui Tu Zhang ◽  
Ling Jun Bu ◽  
Fu Ping Lu
Keyword(s):  
16S Rdna ◽  
Alkaline Protease ◽  
Genetic Manipulation ◽  
Protease Production ◽  
Protease Gene ◽  
Wild Type ◽  
Recombinant Strains ◽  
Manipulation System ◽  
Rdna Sites ◽  
Alkaline Protease Gene

Bacillus alcalophilusisolated was used for the production of alkaline protease. The enzyme encoded by alkaline protease gene (apr4) gene. To further improve the production of the strain for industrial requirement, a genetic manipulation system forBacillus alcalophiluswas developed. Additional copies of theapr4 gene were transferred into the strainBacillus alcalophilusand integrated into the 16S rDNA sites, yielding a series of recombinant strains. One of these recombinant strains, designated K23, exhibited superior properties for production of alkaline protease. the protease activity of K23 achieved by (6.19 ± 0.34) × 104U/ml, which is approximately 111.3% higher than that of the wild-type ones for 50-h fermentation. In addition, the new strain was genetically stable for more than 100 generations. These superior characteristics make it to be more suitable than the wild-type strain for alkaline protease production.

scholarly journals Cloning, characterization, and multiple chromosomal integration of a Bacillus alkaline protease gene.

1991 ◽  
Vol 57 (4) ◽  
pp. 901-909 ◽  
Author(s):  
J C van der Laan ◽  
G Gerritse ◽  
L J Mulleners ◽  
R A van der Hoek ◽  
W J Quax
Keyword(s):  
Alkaline Protease ◽  
Protease Gene ◽  
Chromosomal Integration ◽  
Alkaline Protease Gene

Random Mutation of Alkaline Protease Gene in Bacillus pumilus*

2010 ◽  
Vol 16 (1) ◽  
pp. 96-99
Author(s):  
Jin YANG ◽  
Hong FENG ◽  
Mingyuan WAN ◽  
Huike JIAO ◽  
Xiaolu ZHANG ◽  
...  
Keyword(s):  
Alkaline Protease ◽  
Bacillus Pumilus ◽  
Protease Gene ◽  
Random Mutation ◽  
Alkaline Protease Gene
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