Identification and Algae-Lysing Characteristics of One Algicidal Bacterium FDD1 on Microcystis aeruginosa

In this study, one strain of algicidal bacterium H01 was selected from the water body polluted by Microcystis aeruginosa. It had good removal effects on Microcystis aeruginosa and the removal characteristics on Microcystis aeruginosa were studied. The results indicated that the removal efficiency was connected with such factors as temperature and pH. And the experiment on algicidal mechanism indicated that it lysed the algae cells by secreting metabolites. According to the analysis of its physiological characteristics and 16S rDNA gene sequence, the strain H01 was identified as Brevibacillus parabrevis.

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Physiological responses of Microcystis aeruginosa against the algicidal bacterium Pseudomonas aeruginosa

Ecotoxicology and Environmental Safety ◽

10.1016/j.ecoenv.2016.02.001 ◽

2016 ◽

Vol 127 ◽

pp. 214-221 ◽

Cited By ~ 24

Author(s):

Su Zhou ◽

Hua Yin ◽

Shaoyu Tang ◽

Hui Peng ◽

Donggao Yin ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Microcystis Aeruginosa ◽

Physiological Responses ◽

Algicidal Bacterium

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Algicidal Effect of an Algicidal Bacterium and Preliminary Studies on its Algicidal Mechanism

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.726-731.25 ◽

2013 ◽

Vol 726-731 ◽

pp. 25-28 ◽

Cited By ~ 1

Author(s):

Liang Liu ◽

Xue Zhen Chen ◽

Wen Yi Zhang

Keyword(s):

Microcystis Aeruginosa ◽

Algicidal Bacterium ◽

Algicidal Effect ◽

Bacterium Strain ◽

Algae Control ◽

Technical Basis ◽

Algicidal Effects

In this study, algicidal effects of an algicidal bacterium, strain TL, on Microcystis aeruginosa were studied. The results demonstrate that the bacteria-algae ratio had a significant impact on algicidal effects. Illumination conditions affected the algicidal effects of TL as well. In illumination and darkness cycling, the optimum algicidal effects were realized. Algicidal mechanism of TL was further studied. TL may kill algae through the secretion of certain extracellular nonprotein substance. This research may provide technical basis for biological algae control of Microcystis aeruginosa.

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Biocontrol of Toxin Producing Cyanobacterium Microcystis aeruginosa by Algicidal Bacterium Exiguobacterium acetylicum Strain TM2 Isolated from Mid-Altitudinal Himalayan Lake of Northern India

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2021.1011.020 ◽

2021 ◽

Vol 10 (11) ◽

pp. 170-187

Author(s):

Surabhi Rawat Neetu Shahi ◽

Sumanta Kumar Mallik Richa Pathak ◽

Bhupendra Singh Veena Pande

Keyword(s):

Microcystis Aeruginosa ◽

Morphological Characteristics ◽

Algicidal Activity ◽

Cell Contact ◽

Pathogenicity Test ◽

Northern India ◽

Algicidal Bacterium ◽

Exiguobacterium Acetylicum

Microcystis aeruginosa is a hepatotoxin producing cyanobacteria, found globally in freshwaters. In the present study, an algicidal bacterium against M. aeruginosa was isolated from Bhimtal Lake (29°20’39”N; 79°33’32”E) of Himalayan region of Uttarakhand, India. The isolated bacterium Exiguobacterium acetylicum strainTM2was identified by morphological characteristics, biochemical characteristics and partial 16S ribosomal DNA (rDNA) gene amplification (GenBank accession number: KX155561). Efficacy of E. acetylicum TM2, and its mode of algicidal activity was evaluated against M. aeruginosa. E. acetylicum TM2 showed intense anti-cyanobacterial effect against M. aeruginosa, and approximately 90.0 % death of M. aeruginosa cells were observed after 10 days of incubation. The bacterium attacked the M. aeruginosa cells directly by physically coming in contact and caused damaged to its membrane and internal organelles. Cell free filtrate of E. acetylicum TM2 did not exhibited algicidal activity, which indicates that mode of algicidal mechanism, is cell to cell contact, and not chemically mediated damage by algicidal compounds released from E. acetylicum TM2. Furthermore, in vitro and in vivo pathogenicity test confirm the non-virulence of E. acetylicum TM2 and so it could be potentially useful in mitigation of M. aeruginosa blooms in water.

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Influence of increased nutrient supply on Microcystis aeruginosa at cellular and proteomic levels

Aquatic Microbial Ecology ◽

10.3354/ame01939 ◽

2020 ◽

Vol 85 ◽

pp. 47-58

Author(s):

Y Jiang ◽

Y Liu

Keyword(s):

Microcystis Aeruginosa ◽

Regulatory Protein ◽

Acid Synthesis ◽

Nutrient Supply ◽

Nitrogen Supply ◽

Nitrogen And Phosphorus ◽

Comprehensive Description ◽

Amino Acid Synthesis ◽

High Nutrient ◽

Proteomic Responses

Various studies have observed that increased nutrient supply promotes the growth of bloom-forming cyanobacteria, but only a limited number of studies have investigated the influence of increased nutrient supply on bloom-forming cyanobacteria at the proteomic level. We investigated the cellular and proteomic responses of Microcystis aeruginosa to elevated nitrogen and phosphorus supply. Increased supply of both nutrients significantly promoted the growth of M. aeruginosa and the synthesis of chlorophyll a, protein, and microcystins. The release of microcystins and the synthesis of polysaccharides negatively correlated with the growth of M. aeruginosa under high nutrient levels. Overexpressed proteins related to photosynthesis, and amino acid synthesis, were responsible for the stimulatory effects of increased nutrient supply in M. aeruginosa. Increased nitrogen supply directly promoted cyanobacterial growth by inducing the overexpression of the cell division regulatory protein FtsZ. NtcA, that regulates gene transcription related to both nitrogen assimilation and microcystin synthesis, was overexpressed under the high nitrogen condition, which consequently induced overexpression of 2 microcystin synthetases (McyC and McyF) and promoted microcystin synthesis. Elevated nitrogen supply induced the overexpression of proteins involved in gas vesicle organization (GvpC and GvpW), which may increase the buoyancy of M. aeruginosa. Increased phosphorus level indirectly affected growth and the synthesis of cellular substances in M. aeruginosa through the mediation of differentially expressed proteins related to carbon and phosphorus metabolism. This study provides a comprehensive description of changes in the proteome of M. aeruginosa in response to an increased supply of 2 key nutrients.

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