Identification and Characterization of a Garlic Virus E Genome in Garlic (Allium sativum L.) Using High-Throughput Sequencing from India

Garlic (Allium sativum L.) plants exhibiting mosaics, deformation, and yellow stripes symptoms were identified in Meerut City, Uttar Pradesh, India. To investigate the viruses in the garlic samples, the method of high-throughput sequencing (HTS) was used. Complete genome of the garlic virus E (GarV-E) isolate (NCBI accession No. MW925710) was retrieved. The virus complete genome comprises 8450 nucleotides (nts), excluding the poly (A) tail at the 3′ terminus, with 5′ and 3′ untranslated regions (UTRs) of 99 and 384 nts, respectively, and ORFs encoding replicase with a conserved motif for RNA-dependent RNA polymerase (RdRP), TGB1, TGB2, TGB3, serine-rich protein, coat protein, and nucleic acid binding protein (NABP). The sequence homology shared 83.49–90.40% and 87.48–92.87% with those of GarV-E isolates available in NCBI at the nucleotide and amino acid levels, respectively. Phylogenetic analysis showed a close relationship of this isolate from India (MW925710) with GarV-E isolate YH (AJ292230) from Zhejiang, China. The presence of GarV-E was also confirmed by RT-PCR. The present study is the first report of GarV-E in garlic cultivar Yamuna Safed-3 grown in northern India. However, further studies are needed to confirm its role in symptom development, nationwide distribution, genetic diversity, and potential yield loss to the garlic in India.

Incidence and risk factors of retinopathy of prematurity in extremely low birth weight babies in a tertiary neonatal care unit in northern India

To study the incidence and risk factors of Retinopathy of prematurity in extremely low birth weight babies in a tertiary neonatal care unit in northern India. A prospective cohort study of all neonates born during January 1, 2015 to December 31, 2015, with birth weight less than 1000 grams. Demographic details, risk factors and incidence of ROP were studied. Sixty babies were enrolled for the study with mean birth weight of 892.983±112.933 (560 to 1000) grams and mean gestation age of 29.47±2.258 (25-35) weeks. The incidence of ROP in this cohort was 50% (30 infants), out of which 23% (7 infants) required treatment (laser photocoagulation). The statistical analysis of risk factors on univariate analysis revealed significant association for oxygen exposure, apnoea, surfactant use, anaemia, blood transfusion, intraventricular haemorrhage, sepsis and antibiotic use. On multivariate logistic regression analysis anemia and oxygen exposure > week were found to be independent risk factors for development of ROP. The incidence of ROP was although high in this exclusive cohort of babies born <1000g but there is substantial decrease in incidence as compared to that reported in earlier studies. Gestational age <30 wks, being appropriate for gestation rather than small for gestation, anemia and oxygen exposure>1 week were found to be independent risk factors for development of ROP in this cohort.

Assam

Assam constitutes the region of northeast India bounded by the modern nations of Bangladesh and Bhutan, as well as by the Indian states of Arunachal Pradesh, Bangla, Manipur, Meghalaya, and Nagaland. Known in ancient sources as Prāgjyotiṣpūra (the “city of eastern lights”) and as Kāmarūpa (the “form” or “place of desire”), Assam remains one of the least studied and poorly understood areas of South Asian Hinduism. The home to more than forty recognized tribal communities, Assam has tremendous religious, ethnic, and linguistic diversity, which has helped shape the unique forms of Hinduism that have flourished in the region. Moreover, Assam also has a long reputation as a realm of magic, witchcraft, and the supernatural; for example, even in the early 21st century, the town of Mayong in Morigaon district is infamous as the quintessential “land of black magic.” The historical roots of Hinduism in Assam date back to at least the Varman dynasty of the 4th to 7th centuries, when Vedic sacrifices such as the aśvamedha and other Brahmanical rites were widespread. However, most of the kings of Assam from the Varmans onward came from non-Hindu tribal backgrounds, and the form of Hinduism that developed in the region has long been a complex negotiation between Sanskritic traditions and indigenous practices from the many local communities of the region. During the Assamese Pāla dynasty of the 8th to 12th centuries, Śākta traditions became dominant, and major texts such as the Kālikā Purāṇa were composed, praising the great mother goddess Kāmākhyā (goddess of desire) and her retinue of yoginīs. A unique form of Hindu tantra probably also began to flourish at this time, and Assam has a long reputation as one of the oldest heartlands or perhaps even the original homeland of tantra in South Asia. The Ahom kings of the 13th to 19th centuries continued the patronage of powerful goddesses while also building temples to Śiva, Viṣṇu, and others. During the 16th century, Assam like much of northern India witnessed a powerful revival of Vaiṣṇava bhakti, led by the devotional reformer Śaṅkaradeva (b. 1449–d. 1568). Through Śaṅkaradeva’s influence, Vaiṣṇavism remains a dominant cultural and religious force in Assam to this day. However, even in the 21st century, Assamese Hinduism remains incredibly diverse, and one can still see a wide range of indigenous, folk, and local practices that range from magic and menstruation festivals to spirit possession and ecstatic dance performances.

Rhino Orbital Cerebral Mucormycosis in settings of Covid-19 infection: A Case Series of Thirteen Patients

Background Rhino-Orbital-Cerebral Mucormycosis (ROCM) is an important infectious disease encountered in huge number in this recent post-covid 19 era. An alteration in defence immune system during covid-19 illness, in the presence of uncontrolled hyperglycaemia has led to the new epidemic of ROCM especially in developing nations like India. Method This case series of thirteen patients illustrates the various clinical presentation, laboratorical parameters, imaging features and outcome of patients of ROCM admitted in a tertiary care hospital in Northern India. Result In our case series, a total of 13 newly diagnosed cases of Rhino-Orbital-Cerebral Mucormycosis were studied. History of covid-19 illness was observed in 7 cases (53.8%), use of steroid during Covid-19 illness was seen in 5 cases (38.5%), oxygen therapy was given in 4 cases (30.8%). Co-morbid state in the form of diabetes mellitus was present in 12 cases (92.3%) with mean duration 16.69 months with an important finding of 6 cases (46.2%) having new-onset diabetes; hypertension in present in 3 cases (23.1%). Magnetic resonance imaging of paranasal sinuses showed involvement of multiple sinuses in all the 13 cases(100%), including maxillary and ethmoidal sinuses, with frontal in 12 cases (92.3%), sphenoidal in 11 cases (84.6%), symmetric in 9 cases (69.2%), mastoiditis in 4 cases (30.8%), maxillary space involvement in 4 cases (30.8%), palatal involvement in 1 case (7.7%). Multi-speciality approach treatment was given in the liposomal amphotericin B therapy in all the patients along with thorough endo-nasal debridement done in all cases, transcutaneous retrobulbar amphotericin B in 6 cases (46.2%) with exenteration done in 7 patients (53.9%). At 3 months of follow-up, there was substantial clinical improvement in all the cases. Conclusion There should be definite emphasis on high suspicion of mucor clinically for early diagnosis and aggressive management at initial state of diagnosis for better outcome. The need for sustained proper glycemic control during covid 19 era along with judicious use of steroid and public awareness for early symptoms and manifestations of mucor can curb the magnitude of such potentially opportunistic epidemic to a substantial rate. The longer the infection remains undetected, the greater the devastation ROCM can impose, of which blindness is an important hazard.

A tertiary care prospective of epidemiology and etiopathogenesis of esophageal carcinoma from Northern India

Background: Esophageal cancer is considered to be a one of the most lethal malignancy. Indian population have seen a lot of changes in the epidemiology of this deadly cancer. However very few studies have been done from northern India regarding the epidemiology and etiopathogenesis of this disease. Majority of esophageal cancers (about 90%) are either squamous cell or Adenocarcinoma. Any factor that causes chronic irritation and inflammation of the esophageal mucosa appears to increase the incidence of the esophageal. The aim of the study was to perform an epidemiological study and determine the various factors that are implicated in the pathogenesis of carcinoma esophagus.Methods: This study was conducted in the department of general surgery, Government Medical College, Jammu, as an observational prospective study. It took into account the patients from 2015 to 2020. A total of 23 patients of carcinoma oesophagus who presented to Outdoor patient wing of department of surgery were included in the study. After detailed history, examination and investigations, the various variables were extracted and data was processed.Results: In this study, the majority of people were from rural areas in their seventh decade of life with males: female ratio of 3:1. The majority of people complained of dysphagia and weight loss. The site of tumor was Gastroesophageal junction.Conclusions: This study emphasised the need of health education in our population, especially in young adults to lessen the risk factors for carcinoma esophagus.

Adolescents Hospitalized with SARS-CoV-2: Analysis of Clinical Profile and Risk Factors for Severe Disease over a two-year period

Introduction: There is a lack of studies in adolescents with COVID-19 from developing countries. We aimed to describe the clinical profile and risk factors for severe disease in adolescents hospitalized with COVID-19. Methods: A retrospective analysis of a prospectively admitted cohort of COVID-19 patients was performed at a tertiary hospital in north India. Adolescents aged 12 to 18 years who were hospitalized during the first wave (March 2020 to December 2020) and the second wave (March 2021 to June 2021) of the pandemic were included. Data on the demographic details, clinical presentation, laboratory parameters, disease severity at admission, treatments received, and in-hospital outcomes were retrieved and logistic regression was used to identify the risk factors for occurrence of moderate or severe disease. Results: The study included 197 adolescents with median (IQR) age 15 (13-17) years, of whom 117 (59.4%) were male. Among these, 170 (86.3%) were admitted during the 1st wave. Underlying comorbidities were present in 9 (4.6%) patients. At the time of hospital admission, 60 (30.9%) patients were asymptomatic. In the severity grading, 148 (84.6%) had mild, 16 (9.1%) had moderate, and 11 (6.3%) had severe disease. Fever (14.9%) and cough (14.9%) were the most commonly encountered symptoms. The median (IQR) duration of hospital stay was 10 (8-13) days and 6 (3.1%) patients died in hospital. The odds of moderate to severe disease were 3.8 for second wave, 1.9 for fever and 1.1 for raised C reactive protein (CRP). Conclusion: In our single-center study from northern India, adolescents admitted with COVID-19 had predominantly asymptomatic or mild disease. Admission during the second wave of COVID-19 pandemic, presence of fever and raised CRP were risk factors for moderate or severe disease.

Response of Rice (Oryza sativa L.) Cultivars to Variable Rate of Nitrogen under Wet Direct Seeding in Temperate Ecology

Transplanting rice appears to pose many problems, including depletion of freshwater reservoirs and competition for labor. Conversely, direct seeding allows us to overcome shortcomings associated with conventional transplanting. Nitrogen is a crucial nutrient needed for plant growth and yield. Therefore, this study was executed to analyze the influence of nitrogen on the performance of rice genotypes grown by direct seeding in wet soil. The experiment comprised various rice cultivars, i.e., Shalimar Rice-1, Shalimar Rice-3, Shalimar Rice-4, and Jhelum, and nitrogen (N) levels, i.e., 0, 90, 120, and 150 kg/ha. Shalimar Rice-4 produced a maximum grain yield (6.39 t/ha), followed by Shalimar Rice-3 and Jhelum). The application of 150 kg N/ha showed maximum values for growth parameters, yield attributing traits, and grain yield (6.68 t/ha); however, it remained at par with 120 kg N/ha. Crop water productivity was highest in Shalimar Rice-4 (0.49 kg/m3), and the same showed a consistent increase with increasing N levels from 0–150 kg/ha, with a comparable value of 0.49 to 0.51 recorded at 120 and 150 kg N/ha. Moreover, the Shalimar Rice-1 variety required the maximum in growing degree days (GDD) and helio-thermal units (HTU) to attain different phenological stages till physiological maturity (131 days). However, the cultivar Shalimar rice-4 (SR-4) performed better by registering significantly higher heat use efficiency (HUE) (4.44 kg/ha °C/day). Additionally, the highest net return and the benefit-cost ratio were registered by Shalimar Rice-4. B:C ratio of 1.75 was realized from application of 150 kg N/ha, which remained very close to that achieved with 120 kg N/ha. In conclusion, the rice cultivar Shalimar Rice-4 with the application of 120 kg N/ha could boost rice production under DSR in water-scarce regions of temperate northern India.

Cyclic behavior of late quaternary alluvial soil along Indo-Gangetic Plain: Northern India

The A.D. 1803 and 1934 Bihar-Nepal border earthquake affected Indo-Gangetic Plain with evidences of liquefaction in cities like Patna, Varanasi, Agra, and Delhi in historical past. Recent strong shaking all along the Indo-Gangetic Plains and seismic induced damage to the buildings in Bihar during Mw 7.8 Gorkha earthquake raises the concern for site specific liquefaction potential estimation of alluvial soils. Cyclic triaxial tests were conducted on soil samples from Kanpur, Allahabad, Patna city to know the cyclic behavior, estimate the dynamic soil properties and the effect of relative density, confining pressure and frequency of loading on the cyclic behavior of the soil tested. The test results indicate the cyclic strength of Allahabad soil is less than Patna and Kanpur soil. The Allahabad soil with 80% sand, 10% silt and clay each is more prone to liquefaction than Kanpur soil (82% silt, 16% clay and 2% sand) and Patna soil (10% Kankar, 95% sand, 5% silt). This study indicates soils having sand with silt percentage are more liquefiable than clean sand or silty soil. It can be concluded that the soil of Allahabad and Patna city is more prone to liquefaction than Kanpur soil.

Survival Rate, Postdischarge Readmission Rate, Its Causes and Outcomes Among the Preterm Neonates Admitted in a Tertiary Care Centre, Northern India

Objective: To assess the survival rate, readmission rate, its causes, and outcomes among the preterm neonates that were discharged from the neonatal units of a tertiary care center of northern India. Methods: A retrospective study was conducted in neonatal units of a tertiary care center in northern India. Preterm neonates (<=35 weeks) were admitted in these neonatal units, and their parents were included as participants by total enumeration sampling between July–December 2018. The data was collected from the admission records for the last 6 months from neonatal intensive care unit and from the parents whose babies were discharged. The parents were telephonically interviewed regarding readmissions and mortality. The data relating to the sociodemographic profile of parents, clinical profile of preterm neonates, readmission profile of discharged preterm neonates, and risk predictors for readmission of preterm neonates was collected. Results: A total of 249 preterm neonates were admitted in 6 months. It was found that the survival rate was 197 (79.1%); the readmission rate was 23 (16.4%). Out of total readmitted, majority 22 (95.7) had low maternal education as risk predictor of readmissions, followed by habitats of rural area. Conclusion: Preterm neonates are at more risk for getting multiple readmissions as compared to other neonates. Thus, there is a need to find out the strategies to overcome the burden of readmissions.