Multicentre study of Y chromosome microdeletions in 1,808 Chinese infertile males using multiplex and real-time polymerase chain reaction

Andrologia ◽  
2016 ◽  
Vol 49 (5) ◽  
pp. e12662 ◽  
Author(s):  
X.-B. Zhu ◽  
Y.-H. Gong ◽  
J. He ◽  
A.-L. Guo ◽  
E.-L. Zhi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Multicentre Study ◽  
Real Time ◽  
Y Chromosome ◽  
Chain Reaction ◽  
Y Chromosome Microdeletions ◽  
Polymerase Chain

A Real-Time Polymerase Chain Reaction-Based Protocol for Low/Medium-Throughput Y-Chromosome Microdeletions Analysis

2012 ◽  
Vol 16 (12) ◽  
pp. 1349-1355 ◽  
Author(s):  
Ludovica Segat ◽  
Lara Padovan ◽  
Darja Doc ◽  
Vincenzo Petix ◽  
Marcello Morgutti ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Y Chromosome ◽  
Chain Reaction ◽  
Y Chromosome Microdeletions ◽  
Polymerase Chain

Frequency of Y Chromosome Microdeletions in Azoospermic and Oligospermic Iranian Infertile Men

2020 ◽  
Author(s):  
Sepideh Gholami Yarahmadi ◽  
Saeid Morovvati ◽  
Monireh Raam ◽  
Ziba Morovvati
Keyword(s):  
Polymerase Chain Reaction ◽  
Y Chromosome ◽  
In Vitro Fertilisation ◽  
Control Group ◽  
Chain Reaction ◽  
Azoospermia Factor ◽  
Infertile Men ◽  
Y Chromosome Microdeletions ◽  
Polymerase Chain

Background and Aims: Azoospermia factor (AZF) region of the Ychromosome has several genes which are responsible for normal spermatogenesis. Microdeletions of these genes are associated with azoospermia and oligospermia. These microdeletions are too small to be detected by karyotyping. They can be easily identified using polymerase chain reaction. The aim of this study is to determine the frequencies of Ychromosome microdeletions in azoospermic and oligospermic Iranian infertile men and compare them with other studies in different ethnic groups. Materials and Methods: At first, karyotype analysis was performed in 80 infertile men and 30 healthy age-matched counterparts as control group using standard cytogenetic methods. Second, genomic DNA was extracted from all cases and genetic screening was conducted for Y chromosome microdeletions by multiplex polymerase chain reaction for AZF genes on both infertile and control men using 6 STS markers on the long arm of the Y chromosome. Results: Totally, 49 infertile men were azoospermic and 31 were oligospermic. Y-chromosome microdeletions in the AZFc region were detected in 4 of azoospermic patients. Y-chromosome microdeletions was not detected in any of the oligospermic patients and the control group. Conclusions: This finding recommends that genetic counseling and screening before starting assisted reproductive techniques such as in vitro fertilisation and intracytoplasmic sperm injection can prevent unnecessary treatment and transmission of genetic defects to offspring

scholarly journals Triplex Real-time Polymerase Chain Reaction Optimization for AZF Y-chromosome Microdeletion Analysis

2015 ◽  
Vol 19 (2) ◽  
Author(s):  
Tatiana Puga Torres ◽  
Xavier Blum Rojas ◽  
Medardo Blum Narváez ◽  
Edith López Montanero ◽  
Alexandra Narváez Sarasti
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Y Chromosome ◽  
Chain Reaction ◽  
Y Chromosome Microdeletion ◽  
Reaction Optimization ◽  
Polymerase Chain

Scanning of Y-chromosome azoospermia factors loci using real-time polymerase chain reaction and melting curve analysis

2003 ◽  
Vol 80 (4) ◽  
pp. 907-913 ◽  
Author(s):  
Belén Buch ◽  
José Jorge Galán ◽  
Miguel Lara ◽  
Rocío Ruiz ◽  
Carmen Segura ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Y Chromosome ◽  
Melting Curve ◽  
Curve Analysis ◽  
Melting Curve Analysis ◽  
Chain Reaction ◽  
Polymerase Chain

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain
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