scholarly journals Rapid cryopreservation of small quantities of human spermatozoa by a self‐prepared cryoprotectant without animal component

Andrologia ◽  
2021 ◽  
Author(s):  
Shasha Liu ◽  
Bo Liu ◽  
Wenrui Zhao ◽  
Xiao Liu ◽  
Yang Xian ◽  
...  
Keyword(s):  
Human Spermatozoa ◽  
Animal Component

The Fine Structure of Glycocalyx in Human Spermatozoa. A High Resolution Cytochemical Study

1973 ◽  
Vol 31 ◽  
pp. 640-641
Author(s):  
P. Hernández-Jáuregui ◽  
A. Sosa ◽  
A. González Angulo
Keyword(s):  
Negative Charge ◽  
Iron Hydroxide ◽  
Human Spermatozoa ◽  
Surface Coat ◽  
Cell Surfaces ◽  
Colloidal Iron ◽  
Cytochemical Study ◽  
Specific Permeability ◽  
Extracellular Glycoprotein ◽  
Mammalian Spermatozoa

Glycocalyx is the name given by Bennett to the extracellular glycoprotein coat present in some cell surfaces. It appears to play an important role in cell properties such as antigenicity, cell adhesivity, specific permeability, and ATP ase activity. In the sperm this coat can be directly related to such important phenomena as capacitation and fertilization. The presence of glycocalyx in invertebrate spermatozoa has already been demonstrated. Recently Yanagimachi et al. has determined the negative charges on sperm surfaces of mammalian spermatozoa including man, using colloidal iron hydroxide. No mention was made however of the outer surface coat as composed of substances other than those confering a negative charge. The purpose of this work was therefore to determine the presence of a glycocalyx in human spermatozoa using alcian blue and lanthanum staining.

A High Resolution Cytochemical Study of Nuclear ATPase in Human Spermatozoa

1975 ◽  
Vol 33 ◽  
pp. 594-595
Author(s):  
A. Sosa ◽  
L. Calzada
Keyword(s):  
High Resolution ◽  
Atpase Activity ◽  
Nuclear Membrane ◽  
Human Sperm ◽  
Sperm Nucleus ◽  
Human Spermatozoa ◽  
Cytochemical Study ◽  
Membrane Bound ◽  
Sperm Nuclei ◽  
Interchromatin Space

The dependence of nuclear metabolism on the function of the nuclear membrane is not well understood. Whether or not the function of the nuclear membrane is partial or totally responsible of the repressed template activity of human sperm nucleus has not at present been elucidated. One of the membrane-bound enzymatic activities which is concerned with the mechanisms whereby substances are thought to cross cell membranes is adenosintriphosphatase (ATPase). This prompted its characterization and distribution by high resolution photogrammetry on isolated human sperm nuclei. Isolated human spermatozoa nuclei were obtained as previously described. ATPase activity was demonstrated by the method of Wachstein and Meisel modified by Marchesi and Palade. ATPase activity was identified as dense and irregularly distributed granules confined to the internal leaflet of the nuclear membrane. Within the nucleus the appearance of the reaction product occurs as homogenous and dense precipitates in the interchromatin space.

Membrane fluidity and lipid content of human spermatozoa selected by swim-up method

2001 ◽  
Vol 24 (6) ◽  
pp. 327-334 ◽  
Author(s):  
A. Force ◽  
G. Grizard ◽  
M. N. Giraud ◽  
C. Motta ◽  
B. Sion ◽  
...  
Keyword(s):  
Membrane Fluidity ◽  
Lipid Content ◽  
Human Spermatozoa

Artificial Stimulation of Cryopreserved Human Spermatozoa by Sodium Nitroprusside, 2-Chloroadenosine, and 2-Deoxyadenosine

1997 ◽  
Vol 32 (3) ◽  
pp. 344-352 ◽  
Author(s):  
R.K. Sharma ◽  
A. Agarwal
Keyword(s):  
Sodium Nitroprusside ◽  
Human Spermatozoa ◽  
Stimulation Of

scholarly journals Doing more with less: The flagellar end piece enhances the propulsive effectiveness of human spermatozoa

2020 ◽  
Vol 5 (7) ◽  
Author(s):  
Cara V. Neal ◽  
Atticus L. Hall-McNair ◽  
Jackson Kirkman-Brown ◽  
David J. Smith ◽  
Meurig T. Gallagher
Keyword(s):  
Human Spermatozoa

scholarly journals Clinical benefit for cryopreservation of single human spermatozoa for ICSI: A systematic review and meta‐analysis

Andrology ◽  
2021 ◽  
Author(s):  
Chuan Huang ◽  
Run‐Xin Gan ◽  
Jian‐Lin Hu ◽  
Feng Liu ◽  
Yan Hong ◽  
...  
Keyword(s):  
Systematic Review ◽  
Clinical Benefit ◽  
Meta Analysis ◽  
Human Spermatozoa

Novel assay for determining DNA organization in human spermatozoa: implications for male factor infertility

Urology ◽  
2002 ◽  
Vol 59 (4) ◽  
pp. 575-578 ◽  
Author(s):  
Murali K Ankem ◽  
Eric Mayer ◽  
W.Steven Ward ◽  
Kenneth B Cummings ◽  
Joseph G Barone
Keyword(s):  
Human Spermatozoa ◽  
Male Factor Infertility ◽  
Male Factor ◽  
Dna Organization

Developmental Validation of the SPERM HY-LITERTM Kit for the Identification of Human Spermatozoa in Forensic Samples*,†

2011 ◽  
Vol 56 (4) ◽  
pp. 853-865 ◽  
Author(s):  
Kevin W. P. Miller ◽  
Jennifer Old ◽  
Brian R. Fischer ◽  
Brett Schweers ◽  
Simona Stipinaite ◽  
...  
Keyword(s):  
Human Spermatozoa ◽  
Developmental Validation ◽  
Forensic Samples
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