Effects of adding rotifers on the water quality, plankton composition and growth of Pacific white shrimp, Litopenaeus vannamei juvenile, when cultured with biofloc technology

The effect of two concentrations of an aqueous extract of the San Pedro Daisy, (Lasianthaea podocephala Gray) roots was evaluated on the water quality, physiological condition and production parameters of the white Pacific shrimp, Litopenaeus vannamei (Boone), farmed under intensive laboratory conditions. Two treatments and a control were considered: T1 (1 mL of extract per aquarium), T2 (3 mL of the extract per aquarium) and C (control, 0 mL of the extract). No negative effects of the treatments on water quality parameters were observed, which ranged into acceptable limits and without significant differences (P < 0,05). Some of the production parameters such as final biomass and FCA were slightly better in the treatments using the extract. The concentration of haemolymph metabolites indicate that shrimp from treatments with the extract were under better conditions, suggested by the levels of protein and cholesterol in the muscle; moreover, the gene expression results suggest that the extract could have an immunostimulatory effect on shrimp. However, additional studies are required to evaluate the active ingredients of the root extract at the molecular level in order to have more information for the potential use of the plant.

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Non-specific immune response of Pacific white shrimp Litopenaeus vannamei by supplementation of sodium alginate of Sargassum collected from Lampung Indonesia

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/890/1/012015 ◽

2021 ◽

Vol 890 (1) ◽

pp. 012015

Author(s):

A Setyawan ◽

Riana ◽

Supono ◽

S Hudaidah ◽

H P Fidyandini

Keyword(s):

Water Quality ◽

Immune Response ◽

Litopenaeus Vannamei ◽

Pacific White Shrimp ◽

Specific Immune Response ◽

White Shrimp ◽

Phagocytic Index ◽

White Spot Disease ◽

Randomized Design ◽

Total Plasma Protein

Abstract This study aims to determine the effectiveness of supplementation of sodium (Na) alginate Sargassum sp. from Lampung waters to enhance the non-specific immune response and the resistence of Pacific white shrimp (Litopenaeus vannamei) against white spot disease. This study used a completely randomized design with 3 treatments, namely feeding without alginate supplementation (A) or control, dietary Na alginate Sargassum supplementation at a dose of 2.0 (B), and 4.0 g kg−1 feed (C), each with four replications, for 14 days. Hemolymph sampling was performed on days 0, 7 and 14 to observe the parameter of shrimp hematology included total haemocyte count (THC), phagocytocyte activity (PA), phagocytic index (PI), and total plasma protein (TPP). The hepatopancreas histology profile and water quality were observed at the end of treatment. Data were analyzed by Anova at 95% of confidence interval and continued with Duncan test. Result showedthat the supplementation of Na alginat from Sargassum sp. significantly enhance several shrimp immune response namely THC, PA, and TPP. Meanwhile, the histology of the hepatopancreas of both shrimp treatment and control showed not significantly effect on tissue damage. The water quality was still in normal condition during the treatment. The results of this study indicate that the application of Sargassum alginate supplementation at a dose of 2gr/kg of feed is the best treatment to enhance the immune response of Pacific white shrimp.

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