Evaluation of the basophil activation test and skin prick testing for the diagnosis of sesame food allergy

2018 ◽  
Vol 48 (8) ◽  
pp. 1025-1034 ◽  
Author(s):  
M. Y. Appel ◽  
L. Nachshon ◽  
A. Elizur ◽  
M. B. Levy ◽  
Y. Katz ◽  
...  
Keyword(s):  
Food Allergy ◽  
Skin Prick Testing ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Activation Test

scholarly journals Deallergisation trials of pure celery juice and apple-celery juice mixture by oxidation

2011 ◽  
Vol 29 (No. 2) ◽  
pp. 190-200 ◽  
Author(s):  
P. Novotná ◽  
I. Šetinová ◽  
M. Heroldová ◽  
M. Kmínková ◽  
J. Průchová ◽  
...  
Keyword(s):  
Apple Juice ◽  
No Reduction ◽  
Skin Prick Testing ◽  
Skin Testing ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Enzymatic Browning ◽  
Colour Measurement ◽  
Activation Test ◽  
Natural Polyphenol

This work aimed to determine if it was possible to eliminate or reduce the content of the Api g1 allergen in celery juice by oxidation, utilising its natural polyphenol oxidase (PPO) content. We attempted to determine a possible relationship between the enzymatic browning of celery juice and the reactivity of the Api g1 allergen. Pressed celery juice was stirred, and samples for the colour measurement and allergenicity, determined using the Western Blot (WB) method, were collected at pre-defined times. Oxidation failed to eliminate the allergenicity of pure celery juice. Further trials were focused on celery allergen elimination in apple-celery juices mixtures in ratios of 3:1, 5:1, and 7:1. We selected the 5:1 ratio as the most acceptable from the sensory perspective, and monitored its allergenicity using the WB method, basophil activation test, and skin prick testing. The WB test showed that oxidation, caused by stirring for 120 min, reduced the allergenicity of the mixture. However, the basophil activation test showed no reduction in the allergic response to the oxidised juice mixture. Skin testing showed that the oxidised juice mixture stirred for 120 min exhibited a significantly lower reaction than the juice mixture stirred for 60 min or celery and apple juice stabilised with ascorbic acid. Due to the contradictory results in different tests, the method cannot be declared successful or safe, even for mixtures of apple-celery juices.

scholarly journals Basophil Activation Test in IgE-Mediated Food Allergy: Should We Follow the Flow?

2016 ◽  
Vol 3 (2) ◽  
pp. 158-168 ◽  
Author(s):  
M. A. Faber ◽  
V. Sabato ◽  
I. I. Decuyper ◽  
A. L. Van Gasse ◽  
M. M. Hagendorens ◽  
...  
Keyword(s):  
Food Allergy ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Activation Test ◽  
Ige Mediated

Basophil Activation Test for Diagnosis of Food Allergy

2008 ◽  
Vol 121 (2) ◽  
pp. S250-S250
Author(s):  
V VALDIMARSSON ◽  
S SIGURDARDOTTIR ◽  
I SKAFTADOTTIR ◽  
M CLAUSEN ◽  
B LUDVIGSSON
Keyword(s):  
Food Allergy ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Activation Test

scholarly journals Influence of Antihistamines on Basophil Activation Test in Food Allergy to Milk and Egg

Diagnostics ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 44
Author(s):  
Eleonora Nucera ◽  
Riccardo Inchingolo ◽  
Rosario Nicotra ◽  
Manuela Ferraironi ◽  
Anna Giulia Ricci ◽  
...  
Keyword(s):  
Food Allergy ◽  
Food Challenge ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Double Blind ◽  
Positive Skin ◽  
Activation Test ◽  
History Of ◽  
Ige Mediated ◽  
Food Allergy Diagnosis

Background: The basophil activation test (BAT) is used to improve the accuracy of food allergy diagnosis. To date, the influence of antiallergic drugs on BAT reactivity is poorly investigated. The aim of the study was to investigate if BAT results were influenced by antihistamine intake for 3 months in a cohort of patients with IgE-mediated food allergy to milk or egg. Methods: A retrospective, single-center, observational study was performed. We enrolled subjects with history of hypersensitivity reaction after specific food ingestion, positive skin prick tests and specific IgEs, concomitant allergic rhinitis, and, contraindication to the double-blind, placebo-controlled food challenge due to personal history of systemic reactions related to the ingestion of culprit food. Validated allergens (α-lactoalbumin, β-lactoglobulin, casein, egg white, and yolk) for BAT were used. Results: Thirty-nine patients with well-documented food symptoms and positive allergological workup were included in the study. BAT was positive in 29 patients. The mean percentages of CD63+ expression to specific culprit allergen did not change after the administration of drugs. Conclusions: This was the first study assessing the effects of oral antihistamines on basophil reactivity in cow’s milk and egg food allergy. Antihistamines do not interfere with BAT results.

scholarly journals Egusi seed allergy confirmed using the basophil activation test

2021 ◽  
Author(s):  
Erika Harnik ◽  
Zainab Jama ◽  
Matthew Kwok ◽  
Adam Fox ◽  
Alexandra Santos
Keyword(s):  
Skin Prick Testing ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Food Allergies ◽  
Activation Test ◽  
First Time ◽  
Atopic Child

We report for the first time the case of allergy to egusi seeds in an atopic child of Nigerian origin with allergy to other seeds and nuts. This case highlights the need to know and explore less common foods as potential allergens, the importance of modified skin prick testing and the basophil activation test to support the diagnosis of rare food allergies and of awareness about world cuisine and exotic foods as potential allergens.

Reply to “On the reliability of the CD123-endowed basophil activation test (BAT) and its application in food allergy”

2018 ◽  
Vol 48 (8) ◽  
pp. 1071-1072 ◽  
Author(s):  
Michael Y. Appel ◽  
Liat Nachshon ◽  
Arnon Elizur ◽  
Michael B. Levy ◽  
Yitzhak Katz ◽  
...  
Keyword(s):  
Food Allergy ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Activation Test

scholarly journals Basophil activation test in allergy diagnostics

2020 ◽  
Vol 74 ◽  
pp. 548-555
Author(s):  
Karolina Nowakowska ◽  
Emilia Królewicz ◽  
Andrzej Gamian ◽  
Wojciech Barg
Keyword(s):  
Skin Prick Testing ◽  
Allergic Diseases ◽  
Basophil Activation Test ◽  
Specific Ige ◽  
Basophil Activation ◽  
Diagnostic Methods ◽  
Activation Test ◽  
Allergy Diagnostics ◽  
Continue Research ◽  
Laboratory Tool

The prevalence of allergic diseases in Poland and in the world continues to rise in recent years. The most commonly used methods for diagnosing IgE – dependent allergies are skin prick testing (SPT) and assessment of specific IgE (sIgE) directed against specific allergens. However, both methods have some disadvantages and the obtained results may be inconsistent. In particular, routine diagnostic tests are not always effective for some drugs and foods. Consequently, additional laboratory tools should be used. Basophil activation test (BAT) based on flow cytometry is a promising diagnostic method. The present paper demonstrates the usefulness and effectiveness of BAT protocols in allergy diagnosis in scientific research. In comparison to routinely used diagnostic methods, BAT is an expensive and complicated laboratory tool. However, it offers the possibility to efficiently and effectively recognize allergies. Introducing BAT into routine diagnostics in allergology is problematic because this method has not yet been standardized and validated. Therefore, there is a justified need to continue research in this field. If standardized and validated, BAT may offer a reasonable improvement in allergy diagnostics in the future.

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