Assessment of mating competitiveness of sterile Ceratitis capitata in laboratory and field cage tests in northern Iran

2021 ◽  
Author(s):  
Mehrdad Ahmadi ◽  
Shiva Osouli ◽  
Adly Mohamed Mohamed Abd‐Alla ◽  
Nadia Kalantarian
Keyword(s):  
Ceratitis Capitata ◽  
Field Cage ◽  
Northern Iran ◽  
Mating Competitiveness

scholarly journals Physalis peruviana L. (Solanaceae) Is Not a Host of Ceratitis capitata (Diptera: Tephritidae): Evidence from Multi-Year Field and Laboratory Studies in Colombia

Insects ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 434
Author(s):  
Martín Aluja ◽  
Larissa Guillén ◽  
Ángela Castro ◽  
Martha Liliana Cárdenas ◽  
Maribel Hurtado ◽  
...  
Keyword(s):  
Confidence Level ◽  
Ceratitis Capitata ◽  
Choice Experiments ◽  
Laboratory Studies ◽  
Ripe Fruit ◽  
Field Cage ◽  
Physalis Peruviana ◽  
Host Status ◽  
Gravid Females ◽  
Regulatory Decisions

Scientifically-based, tephritid fly host status determination lies at the heart of strategic regulatory decisions impinging on international fruit trade. Here we conducted intensive field and laboratory studies with peaches as controls, to determine the host status of Physalis peruviana for the Medfly—Ceratitis capitata, as this fruit is experiencing a consumption boom worldwide. A total of 98,132 Uchuvas (local name), collected in Colombia from the plant or the ground over a three-year period (2016–2018) did not yield a single C. capitata larva or pupa, thus reaching a Probit 9 level with 99.9968% efficacy and 96% confidence level. Field-cage studies with enclosed fruit-bearing Uchuva plants, exposing fruit with an intact, damaged or totally removed husk to the attack of C. capitata, also failed to yield infestations. Highly artificial choice experiments, exposing gravid females to unripe and fully ripe fruit, resulted in an absence of infestations, even when overripe Uchuvas were artificially damaged. The husk and surface resins/waxes inhibit fly landings on fruit and oviposition activity. Considering our results and the fact that the foliage, husk and fruit of P. peruviana are repellent/toxic to insects, we conclude that this plant should be treated as a non-natural and non-conditional host of C. capitata.

The Sterile Insect Release Method and the Importance of Thermal Conditioning Before Release - Field-Cage Experiments With Dacus-Tryoni in Spring Weather

1987 ◽  
Vol 35 (2) ◽  
pp. 197 ◽  
Author(s):  
HAC Fay ◽  
A Meats
Keyword(s):  
Target Population ◽  
Cold Weather ◽  
Large Field ◽  
Field Cage ◽  
Mating Competitiveness ◽  
Rate Of Increase ◽  
Conditioned State ◽  
Males And Females ◽  
Sterile Insect Release ◽  
Release Method

We present a rationale for the strategy of suppressing the fertility and hence the rate of increase of target populations in spring by the release of sterile insects. Insects for mass release are normally reared in warm conditions and are potentially unsuitable for use in cold weather. A comparison was made of the effectiveness of warm- and cold-conditioned sterile flies in experiments in large field-cages during three successive spring seasons. Sterile males and females, and wild males and females were released into the cages in the ratio 2:2:1:1, respectively. Warm-conditioned sterile flies survived poorly between release and mating. Their mating competitiveness was inferior to that of the wild flies, and they were relatively ineffective in reducing their fertility. Sterile flies released in a cold-conditioned state (equal to that of the wild flies), survived as well as wild flies, and suppressed their fertility to an extent which indicates that their mating competitiveness can be equal to that of the wild flies, if the weather is not too severe. The concept of total competitiveness is introduced. If competitiveness is calculated by means of the ratio of sterile to wild flies that exists at release (rather than at mating), the resulting value is a measure of the potential of the sterile flies to survive to mating age, and to mate in competition with the wild flies. This value is especially pertinent to situations such as a puparial release, when there is a delay between release and mating. In such circumstances, it is needed for calculating the required ratio of sterile to wild insects to achieve the desired reduction of fertility (and thus rate of increase) in the target population.

Mating Competitiveness of Culex tarsalis1 Double Translocation Heterozygote Males in Laboratory and Field Cage Trials3

1977 ◽  
Vol 70 (6) ◽  
pp. 849-854 ◽  
Author(s):  
H. A. Terwedow ◽  
S. M. Asman ◽  
P. T. McDonald ◽  
R. L. Nelson ◽  
W. C. Reeves
Keyword(s):  
Field Cage ◽  
Translocation Heterozygote ◽  
Mating Competitiveness

scholarly journals Male sexual competitiveness of two Ceratitis capitata strains, tsl Vienna 8 and OX3864A transgenics, in field cage conditions

2017 ◽  
Vol 164 (3) ◽  
pp. 318-326 ◽  
Author(s):  
Jair F. Virginio ◽  
Maylen Gómez ◽  
Aline M. Pinto ◽  
Gessyca G. Aniely ◽  
Beatriz J. Paranhos ◽  
...  
Keyword(s):  
Ceratitis Capitata ◽  
Field Cage

scholarly journals Comportamento Reprodutivo de Ceratitis capitata Wiedemann (Diptera: Tephritidae): Efeito do Tamanho dos Machos Sobre o Seu Sucesso de Cópula.

2012 ◽  
Vol 5 (3) ◽  
pp. 190-197
Author(s):  
Alberto Moreira Silva Neto ◽  
Vanessa Simões Dias ◽  
Iara Sordi Joachim-Bravo
Keyword(s):  
Size Effect ◽  
Reproductive Behavior ◽  
Ceratitis Capitata ◽  
Gradual Increase ◽  
Small Male ◽  
Adult Males ◽  
Male Size ◽  
Sexual Pheromone ◽  
Field Cage ◽  
Pheromone Emission

Neste trabalho avaliou-se a influência do tamanho de machos de Ceratitis capitata (Wiedemann) no seu sucesso de cópula. Para assegurar a produção de machos adultos de diferentes tamanhos (grandes e pequenos), dois grupos de larvas foram alimentados com diferentes concentrações de proteína. Subsequentemente, machos adultos de ambos os grupos foram comparados em termos de seu sucesso de cópula (estimado pela capacidade de ser escolhido pela fêmea) e de quantidade de machos que emitiram feromônio. O sucesso de cópula em laboratório foi avaliado com machos em várias proporções, nas quais se manteve constante o número de machos grandes com cinco dias de idade (um único macho) em relação a um aumento progressivo de machos pequenos com mesma idade. As proporções testadas foram 1:1, 1:2, 1:3, 1:4, 1:5, e 1:10. Nos experimentos de sucesso de cópula na proporção 1:1 e nos de emissão de feromônio, foram testados machos pequenos de diferentes idades (cinco, nove ou 13 dias), enquanto que a idade dos machos grandes se manteve constante (cinco dias). Experimentos de sucesso de cópula na proporção 1:1 também foram realizados em gaiola de campo. Evidenciou-se que os machos grandes levaram vantagem em todos os parâmetros analisados em laboratório, emitindo mais feromônio e tendo um maior sucesso de cópula, mesmo quando a idade dos machos pequenos foi variada. O efeito de tamanho foi tão significativo, que na proporção de 1 macho grande para 10 machos pequenos, as fêmeas ainda escolheram os machos grandes. Em gaiola de campo os resultados foram similares aos de laboratório. Reproductive Behavior of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae): Effect of the Male Size on the Copula Success Abstract. This work evaluated the influence of size on the copula success in Ceratitis capitata (Wiedemann). To assure the production of different adult sizes (big and small), two groups of larvae had been fed with different protein concentrations. Subsequently, adult males of both groups had been compared in terms of copula success and amount of males who showed the first step of courtship (emission of sexual pheromone). The copula success in laboratory was evaluated with males in some ratios, which the number of big males with five days of life (an unique male) in relation to a gradual increase of small males with same age kept constant. The tested ratios had been 1:1, 1:2, 1:3, 1:4, 1:5, and 1:10. In the experiments of copula success in the 1:1 ratio and the ones of pheromone emission, they had been tested small male of different ages (five, nine or 13 days), whereas the age of the big males kept constant (five days). Experiments of copula success in the 1:1 ratio had been also carried through in field cage. It was prove that the big males had taken advantage in all the parameters analyzed in laboratory, emitting pheromone and having a bigger copula success, exactly when the age of the small males was varied. The size effect was so significant, that in the ratio of 1 big male for 10 small males, the females had still chosen the big males. In field cage, the results had been similar to the ones of laboratory.

Asymmetries in Physiological State as a Possible Cause of Resident Advantage in Contests

Behaviour ◽  
1998 ◽  
Vol 135 (8) ◽  
pp. 1013-1030 ◽  
Author(s):  
Russell Messing ◽  
Daniel Papaj
Keyword(s):  
Ceratitis Capitata ◽  
Physiological State ◽  
Egg Laying ◽  
Field Cage ◽  
Searching Behaviour ◽  
Relative Age ◽  
State Variable ◽  
Dynamical State ◽  
Host Fruit ◽  
Possible Cause

AbstractAs is common in defense of resources in many animals, contests on host fruit between female Mediterranean fruit flies (Ceratitis capitata) are generally resolved in favour of the resident individual. Here we offer an interpretation of resident advantage in this species which is derived from a dynamical state-variable perspective on behaviour. We first demonstrated the occurrence of residence advantage. In field-cage assays of freely-foraging and freely-interacting females within a tree bearing host coffee berries, the occurrence of two females on a berry almost always resulted in contests. Approximately half of the contests among females on berries resulted in clear winners; resident females won an overwhelming majority of such contests. Contests tended to occur while the resident fly was currently engaged in egg-laying, either boring into the berry with their ovipositor or marking the berry after laying eggs. Non-residents, by contrast, were uniformly engaged in searching behaviour. We next tested the hypothesis that degree of resident advantage is a function of the degree to which a resident is engaged in egg-laying behaviour just prior to the contest. In experimental manipulations of resident status, which female won depended strongly on what residents were doing at the time contests were initiated. If residents were resting and grooming, non-residents (who were uniformly searching) usually won. If residents were laying eggs or marking the fruit after laying eggs, residents usually won. Only when both residents and non-residents were engaged in searching behaviour did the outcome of a contest not depend on resident status. Finally, we tested the hypothesis that contest outcome is related to the dynamical state of the contestants in terms of their relative age. We staged contests between host-deprived females that differed in age by two weeks, manipulating age independently of resident status. Older females won virtually all contests with younger ones regardless of resident status. In a field-cage assay of freely-foraging flies, older females also engaged in significantly more egg-laying activity. Taken together, these results indicate that older females value a fruit more highly than do younger ones, invest more in defense of that resource and therefore win more contests.

scholarly journals Genetic structure and symbiotic profile of worldwide natural populations of the Mediterranean fruit fly, Ceratitis capitata

BMC Genetics ◽  
2020 ◽  
Vol 21 (S2) ◽  
Author(s):  
Katerina Nikolouli ◽  
Antonios A. Augustinos ◽  
Panagiota Stathopoulou ◽  
Elias Asimakis ◽  
Anastasios Mintzas ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Bacterial Communities ◽  
Ceratitis Capitata ◽  
Natural Populations ◽  
Fruit Fly ◽  
Mediterranean Fruit Fly ◽  
Wild Populations ◽  
Mating Competitiveness ◽  
Biological Quality ◽  
The Mediterranean

Abstract Background The Mediterranean fruit fly, Ceratitis capitata, is a cosmopolitan agricultural pest of worldwide economic importance and a model for the development of the Sterile Insect Technique (SIT) for fruit flies of the Tephritidae family (Diptera). SIT relies on the effective mating of laboratory-reared strains and natural populations, and therefore requires an efficient mass-rearing system that will allow for the production of high-quality males. Adaptation of wild flies to an artificial laboratory environment can be accompanied by negative effects on several life history traits through changes in their genetic diversity and symbiotic communities. Such changes may lead to reduced biological quality and mating competitiveness in respect to the wild populations. Profiling wild populations can help understand, and maybe reverse, deleterious effects accompanying laboratory domestication thus providing insects that can efficiently and effectively support SIT application. Results In the present study, we analyzed both the genetic structure and gut symbiotic communities of natural medfly populations of worldwide distribution, including Europe, Africa, Australia, and the Americas. The genetic structure of 408 individuals from 15 distinct populations was analyzed with a set of commonly used microsatellite markers. The symbiotic communities of a subset of 265 individuals from 11 populations were analyzed using the 16S rRNA gene-based amplicon sequencing of single individuals (adults). Genetic differentiation was detected among geographically distant populations while adults originated from neighboring areas were genetically closer. Alpha and beta diversity of bacterial communities pointed to an overall reduced symbiotic diversity and the influence of the geographic location on the bacterial profile. Conclusions Our analysis revealed differences both in the genetic profile and the structure of gut symbiotic communities of medfly natural populations. The genetic analysis expanded our knowledge to populations not analyzed before and our results were in accordance with the existing scenarios regarding this species expansion and colonization pathways. At the same time, the bacterial communities from different natural medfly populations have been characterized, thus broadening our knowledge on the microbiota of the species across its range. Genetic and symbiotic differences between natural and laboratory populations must be considered when designing AW-IPM approaches with a SIT component, since they may impact mating compatibility and mating competitiveness of the laboratory-reared males. In parallel, enrichment from wild populations and/or symbiotic supplementation could increase rearing productivity, biological quality, and mating competitiveness of SIT-important laboratory strains.

scholarly journals Genetics and Mating Competitiveness of Ceratitis capitata (Diptera: Tephritidae) Strains Carrying the Marker Sergeant, Sr 2

2005 ◽  
Vol 98 (1) ◽  
pp. 119-125 ◽  
Author(s):  
N. Niyazi ◽  
C. Caceres ◽  
A. Delprat ◽  
V. Wornoayporn ◽  
E. Ramirez Santos ◽  
...  
Keyword(s):  
Ceratitis Capitata ◽  
Mating Competitiveness

scholarly journals Developing Field Cage Tests to Measure Mating Competitiveness of Sterile Light Brown Apple Moths (Lepidoptera: Tortricidae) in Western Australia

2016 ◽  
Vol 99 (sp1) ◽  
pp. 138-145 ◽  
Author(s):  
Bill Woods ◽  
Donald McInnis ◽  
Ernie Steiner ◽  
Alven Soopaya ◽  
Jeremy Lindsey ◽  
...  
Keyword(s):  
Western Australia ◽  
Field Cage ◽  
Mating Competitiveness
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