Adenosine A2A receptors modulate the dopamine D2 receptor-mediated inhibition of synaptic transmission in the mouse prefrontal cortex

2018 ◽  
Vol 47 (9) ◽  
pp. 1127-1134 ◽  
Author(s):  
Joana I. Real ◽  
Ana Patrícia Simões ◽  
Rodrigo A. Cunha ◽  
Samira G. Ferreira ◽  
Daniel Rial
Keyword(s):  
Prefrontal Cortex ◽  
Synaptic Transmission ◽  
Dopamine D2 Receptor ◽  
D2 Receptor ◽  
Adenosine A2a Receptors ◽  
A2a Receptors ◽  
Dopamine D2 ◽  
Adenosine A2a

scholarly journals Increased desensitization of dopamine D2 receptor-mediated response in the ventral tegmental area in the absence of adenosine A2A receptors

Neuroscience ◽  
2011 ◽  
Vol 190 ◽  
pp. 103-111 ◽  
Author(s):  
R. Al-Hasani ◽  
J.D. Foster ◽  
A. Metaxas ◽  
C. Ledent ◽  
S.M.O. Hourani ◽  
...  
Keyword(s):  
Ventral Tegmental Area ◽  
Dopamine D2 Receptor ◽  
D2 Receptor ◽  
Adenosine A2a Receptors ◽  
A2a Receptors ◽  
Dopamine D2 ◽  
Ventral Tegmental ◽  
Adenosine A2a

scholarly journals Dopamine D2 and Adenosine A2A Receptors Regulate NMDA-Mediated Excitation in Accumbens Neurons Through A2A–D2 Receptor Heteromerization

2008 ◽  
Vol 34 (4) ◽  
pp. 972-986 ◽  
Author(s):  
Karima Azdad ◽  
David Gall ◽  
Amina S Woods ◽  
Catherine Ledent ◽  
Sergi Ferré ◽  
...  
Keyword(s):  
D2 Receptor ◽  
Adenosine A2a Receptors ◽  
A2a Receptors ◽  
Dopamine D2 ◽  
Adenosine A2a

scholarly journals Allosteric mechanisms within the adenosine A2A–dopamine D2 receptor heterotetramer

2016 ◽  
Vol 104 ◽  
pp. 154-160 ◽  
Author(s):  
Sergi Ferré ◽  
Jordi Bonaventura ◽  
Dardo Tomasi ◽  
Gemma Navarro ◽  
Estefanía Moreno ◽  
...  
Keyword(s):  
Dopamine D2 Receptor ◽  
D2 Receptor ◽  
Dopamine D2 ◽  
Allosteric Mechanisms ◽  
Adenosine A2a

scholarly journals Essential Control of the Function of the Striatopallidal Neuron by Pre-coupled Complexes of Adenosine A2A-Dopamine D2 Receptor Heterotetramers and Adenylyl Cyclase

2018 ◽  
Vol 9 ◽  
Author(s):  
Sergi Ferré ◽  
Jordi Bonaventura ◽  
Wendy Zhu ◽  
Candice Hatcher-Solis ◽  
Jaume Taura ◽  
...  
Keyword(s):  
Adenylyl Cyclase ◽  
Dopamine D2 Receptor ◽  
D2 Receptor ◽  
Dopamine D2 ◽  
Adenosine A2a

scholarly journals Revealing Adenosine A2A-Dopamine D2 Receptor Heteromers in Parkinson’s Disease Post-Mortem Brain through a New AlphaScreen-Based Assay

2019 ◽  
Vol 20 (14) ◽  
pp. 3600 ◽  
Author(s):  
Víctor Fernández-Dueñas ◽  
Maricel Gómez-Soler ◽  
Marta Valle-León ◽  
Masahiko Watanabe ◽  
Isidre Ferrer ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Dynamic Range ◽  
Dopamine D2 Receptor ◽  
D2 Receptor ◽  
Post Mortem ◽  
Dopamine D2 ◽  
Post Mortem Brain ◽  
Receptor Heteromers ◽  
Adenosine A2a

Background: Several biophysical techniques have been successfully implemented to detect G protein-coupled receptors (GPCRs) heteromerization. Although these approaches have made it possible to ascertain the presence of GPCR heteromers in animal models of disease, no success has been accomplished in pathological human post-mortem brains. The AlphaScreen technology has been consistently used to quantify small analyte accumulation or depletion, bimolecular interactions, and post-translational modifications. The high signal-to-background, dynamic range and sensitivity exhibited by this technology support that it may be suitable to detect GPCR heteromers even under non-optimal conditions. Methods: Here, we describe the development of a new AlphaScreen assay to detect GPCR oligomers in human post-mortem brain. Results: Adenosine A2A-dopamine D2 receptor (A2AR/D2R) heteromer formation was monitored in caudate from healthy and Parkinson’s disease (PD) subjects. The approach was first validated using striatal membranes from wild type and A2AR deficient mice. Secondly, we took advantage of the 6-hydroxydopamine hemiparkinsonian rat model to validate previous results. In addition, finally, A2AR/D2R heteromer formation was assessed in caudate membranes from human post-mortem brains. Importantly, our preliminary results revealed an increase in A2AR/D2R heteromer formation in PD brains. Conclusions: The new AlphaScreen assay allowed assessing GPCR heteromers in human post-mortem brains with high sensitivity.

Actions of Adenosine A2A Receptors on Synaptic Connections of Spiny Projection Neurons in the Neostriatal Inhibitory Network

2008 ◽  
Vol 99 (4) ◽  
pp. 1884-1889 ◽  
Author(s):  
Tomomi Shindou ◽  
Gordon W. Arbuthnott ◽  
Jeffery R. Wickens
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Synaptic Transmission ◽  
Projection Neurons ◽  
Synaptic Connections ◽  
Adenosine A2a Receptors ◽  
A2a Receptors ◽  
Cells Of Origin ◽  
Adenosine A2a ◽  
Whole Cell Recordings

There is growing evidence that adenosine plays a crucial role in basal ganglia function, particularly in the modulation of voluntary movement. An adenosine-based treatment for Parkinson's disease shows promise in recent clinical studies. Adenosine A2A receptors, the receptors involved in this treatment, are highly expressed in the neostriatum. Previous studies have suggested opposing actions of these receptors on synaptic transmission at striatal and pallidal terminals of the same spiny projection neurons, but the cells of origin of the intrastriatal terminals mediating these actions have not been identified. We used dual whole cell recordings to record simultaneously from pairs of striatal cells; this enabled definitive identification of the presynaptic and postsynaptic cells mediating the effects of A2A receptors. We found that A2A receptors facilitate GABAergic synaptic transmission by intrastriatal collaterals of the spiny projection neurons, consistent with their previously reported actions on synaptic transmission at pallidal terminals. This neuromodulatory action on lateral inhibition in the striatum may underlie, in part, the therapeutic efficacy of adenosine-based treatments for Parkinson's disease.

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