Evidence for the Existence of Antagonistic Intramembrane Adenosine A2a/dopamine D2 Receptor Interactions in the Basal Ganglia: Analysis from the Network to the Molecular Level

1995 ◽  
pp. 499-507 ◽  
Author(s):  
Kjell Fuxe ◽  
Sergi Ferré ◽  
Sarmila Dasgupta ◽  
William T. O’Connor ◽  
Per Snaprud ◽  
...  
Keyword(s):  
Basal Ganglia ◽  
Molecular Level ◽  
Dopamine D2 Receptor ◽  
D2 Receptor ◽  
Dopamine D2 ◽  
Receptor Interactions ◽  
Adenosine A2a

scholarly journals Allosteric mechanisms within the adenosine A2A–dopamine D2 receptor heterotetramer

2016 ◽  
Vol 104 ◽  
pp. 154-160 ◽  
Author(s):  
Sergi Ferré ◽  
Jordi Bonaventura ◽  
Dardo Tomasi ◽  
Gemma Navarro ◽  
Estefanía Moreno ◽  
...  
Keyword(s):  
Dopamine D2 Receptor ◽  
D2 Receptor ◽  
Dopamine D2 ◽  
Allosteric Mechanisms ◽  
Adenosine A2a

scholarly journals Conditional Ablation of Striatal Neuronal Types Containing Dopamine D2 Receptor Disturbs Coordination of Basal Ganglia Function

2003 ◽  
Vol 23 (27) ◽  
pp. 9078-9088 ◽  
Author(s):  
Hiromi Sano ◽  
Yasunobu Yasoshima ◽  
Natsuki Matsushita ◽  
Takeshi Kaneko ◽  
Kenji Kohno ◽  
...  
Keyword(s):  
Basal Ganglia ◽  
Dopamine D2 Receptor ◽  
D2 Receptor ◽  
Dopamine D2 ◽  
Neuronal Types

scholarly journals Revealing Adenosine A2A-Dopamine D2 Receptor Heteromers in Parkinson’s Disease Post-Mortem Brain through a New AlphaScreen-Based Assay

2019 ◽  
Vol 20 (14) ◽  
pp. 3600 ◽  
Author(s):  
Víctor Fernández-Dueñas ◽  
Maricel Gómez-Soler ◽  
Marta Valle-León ◽  
Masahiko Watanabe ◽  
Isidre Ferrer ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Dynamic Range ◽  
Dopamine D2 Receptor ◽  
D2 Receptor ◽  
Post Mortem ◽  
Dopamine D2 ◽  
Post Mortem Brain ◽  
Receptor Heteromers ◽  
Adenosine A2a

Background: Several biophysical techniques have been successfully implemented to detect G protein-coupled receptors (GPCRs) heteromerization. Although these approaches have made it possible to ascertain the presence of GPCR heteromers in animal models of disease, no success has been accomplished in pathological human post-mortem brains. The AlphaScreen technology has been consistently used to quantify small analyte accumulation or depletion, bimolecular interactions, and post-translational modifications. The high signal-to-background, dynamic range and sensitivity exhibited by this technology support that it may be suitable to detect GPCR heteromers even under non-optimal conditions. Methods: Here, we describe the development of a new AlphaScreen assay to detect GPCR oligomers in human post-mortem brain. Results: Adenosine A2A-dopamine D2 receptor (A2AR/D2R) heteromer formation was monitored in caudate from healthy and Parkinson’s disease (PD) subjects. The approach was first validated using striatal membranes from wild type and A2AR deficient mice. Secondly, we took advantage of the 6-hydroxydopamine hemiparkinsonian rat model to validate previous results. In addition, finally, A2AR/D2R heteromer formation was assessed in caudate membranes from human post-mortem brains. Importantly, our preliminary results revealed an increase in A2AR/D2R heteromer formation in PD brains. Conclusions: The new AlphaScreen assay allowed assessing GPCR heteromers in human post-mortem brains with high sensitivity.

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