scholarly journals Efficient flavinylation of glycosomal fumarate reductase by its own ApbE domain in Trypanosoma brucei

FEBS Journal ◽  
2021 ◽  
Author(s):  
Robin Schenk ◽  
Sabine Bachmaier ◽  
Frédéric Bringaud ◽  
Michael Boshart
Keyword(s):  
Trypanosoma Brucei ◽  
Fumarate Reductase

scholarly journals The role of succinate in the respiratory chain of Trypanosoma brucei procyclic trypomastigotes

1989 ◽  
Vol 259 (2) ◽  
pp. 363-368 ◽  
Author(s):  
J F Turrens
Keyword(s):  
Cytochrome C ◽  
Trypanosoma Brucei ◽  
Respiratory Chain ◽  
Intact Cell ◽  
Inhibitory Effect ◽  
Fumarate Reductase ◽  
Glycerol Phosphate ◽  
Intact Cells ◽  
Fluorescence Studies ◽  
Nadh Cytochrome

Trypanosoma brucei procyclic trypomastigotes were made permeable by using digitonin (0-70 micrograms/mg of protein). This procedure allowed exposure of coupled mitochondria to different substrates. Only succinate and glycerol phosphate (but not NADH-dependent substrates) were capable of stimulating oxygen consumption. Fluorescence studies on intact cells indicated that addition of succinate stimulates NAD(P)H oxidation, contrary to what happens in mammalian mitochondria. Addition of malonate, an inhibitor of succinate dehydrogenase, stimulated NAD(P)H reduction. Malonate also inhibited intact-cell respiration and motility, both of which were restored by further addition of succinate. Experiments carried out with isolated mitochondrial membranes showed that, although the electron transfer from succinate to cytochrome c was inhibitable by antimycin, NADH-cytochrome c reductase was antimycin-insensitive. We postulate that the NADH-ubiquinone segment of the respiratory chain is replaced by NADH-fumarate reductase, which reoxidizes the mitochondrial NADH and in turn generates succinate for the respiratory chain. This hypothesis is further supported by the inhibitory effect on cell growth and respiration of 3-methoxyphenylacetic acid, an inhibitor of the NADH-fumarate reductase of T. brucei.

scholarly journals Metabolic selection of a homologous recombination-mediated gene loss protects Trypanosoma brucei from ROS production by glycosomal fumarate reductase

2021 ◽  
Vol 296 ◽  
pp. 100548
Author(s):  
Marion Wargnies ◽  
Nicolas Plazolles ◽  
Robin Schenk ◽  
Oriana Villafraz ◽  
Jean-William Dupuy ◽  
...  
Keyword(s):  
Homologous Recombination ◽  
Trypanosoma Brucei ◽  
Gene Loss ◽  
Fumarate Reductase ◽  
Ros Production ◽  
Selection Of

Enhanced succinic acid production in Aspergillus saccharolyticus by heterologous expression of fumarate reductase from Trypanosoma brucei

2015 ◽  
Vol 100 (4) ◽  
pp. 1799-1809 ◽  
Author(s):  
Lei Yang ◽  
Mette Lübeck ◽  
Birgitte K. Ahring ◽  
Peter S. Lübeck
Keyword(s):  
Heterologous Expression ◽  
Succinic Acid ◽  
Trypanosoma Brucei ◽  
Acid Production ◽  
Fumarate Reductase ◽  
Succinic Acid Production

Screening of some Asteraceae to discover new active compounds against Trypanosoma brucei by metabolite profiling and PLS analysis

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
MS Nogueira ◽  
FB da Costa ◽  
MA Magenta ◽  
M Kaiser ◽  
R Brun ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Metabolite Profiling ◽  
Active Compounds ◽  
Pls Analysis

Alkaloid subfractions of Buxus sempervirens L. leaves with strong in vitro activity against Trypanosoma brucei rhodesiense

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
JB Althaus ◽  
G Jerz ◽  
P Winterhalter ◽  
M Kaiser ◽  
R Brun ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Trypanosoma Brucei Rhodesiense ◽  
Buxus Sempervirens
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