Comparison of genetic diversity at microsatellite loci in near-extinct and non-endangered species of Mexican goodeine fishes and prediction of cross-amplification within the family

Abstract Fourteen novel microsatellite loci are described and characterized in two species of electric eels, Electrophorus variiand E. voltaifrom floodplains and rivers of the Amazon rainforest. These loci are polymorphic, highly informative, and have the capacity to detect reliable levels of genetic diversity. Likewise, the high combined probability of paternity exclusion value and low combined probability of genetic identity value obtained demonstrate that the new set of loci displays suitability for paternity studies on electric eels. In addition, the cross-amplification of electric eel species implies that it may also be useful in the study of the closely related E. electricus, and to other Neotropical electric fishes (Gymnotiformes) species as tested herein.

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Development of microsatellite loci and population genetics in the bumblebee catfish species Pseudopimelodus atricaudus and Pseudopimelodus magnus (Siluriformes: Pseudopimelodidae)

Neotropical Ichthyology ◽

10.1590/1982-0224-2020-0053 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

José David Rangel-Medrano ◽

Edna Judith Márquez

Keyword(s):

Genetic Diversity ◽

Population Genetics ◽

Population Structure ◽

Genetic Differentiation ◽

River Basin ◽

Microsatellite Loci ◽

Population Genetic ◽

High Genetic Diversity ◽

The Family ◽

The Mean

ABSTRACT The Neotropical freshwater catfish Pseudopimelodus atricaudus and Pseudopimelodus magnus are two recently discovered species endemic to the Colombian Magdalena-Cauca River basin. In this study, a set of 13 microsatellite loci were developed by using next generation sequence technology to assess the genetic diversity and population structure in P. atricaudus and test for cross-species amplification in P. magnus. Both species exhibited high genetic diversity (P. atricaudus: Na: 9.000 - 9.769 alleles/locus, Ho: 0.760 - 0.804, HE: 0.804 - 0.840; P. magnus: Na: 12.8 - 5.4 alleles/locus, Ho: 0.638 - 0.683, HE: 0.747 - 0.755) compared to the mean levels of genetic diversity reported for Neotropical Siluriformes, and lack of genetic differentiation among sampling sites within the Cauca River (P. atricaudus: F’ST=0.013 - 0.017, P > 0.05, D’est= -0.004 - 0.023, P > 0.05; P. magnus: F’ST= 0.031, P= 0.055; D’est= 0.045, P= 0.058). This work is the first insight on the diversity and the population genetics of species of the family Pseudopimelodidae and provides a framework to further population genetic and conservation analyses needed in this poorly studied family at the microevolutionary level.

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Supplementary data for: Polymorphic Microsatellite Loci From an Indigenous Asian Fungus-Growing Termite, Macrotermes gilvus (Blattodea: Termitidae) and Cross Amplification in Related Taxa

Environmental Entomology ◽

10.1603/en11228st1 ◽

2012 ◽

Vol 41 (2) ◽

Author(s):

G. Singham

Keyword(s):

Microsatellite Loci ◽

Supplementary Data ◽

Cross Amplification ◽

Polymorphic Microsatellite

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Assessment of the genetic diversity of microsatellite loci of horses of Heavy Draft breeds

Genetika i razvedenie zhivotnyh ◽

10.31043/2410-2733-2018-2-39-44 ◽

2018 ◽

pp. 39-44

Author(s):

N. Blohina ◽

◽

L. Khrabrova ◽

A. Zaitcev ◽

I. Gavrilicheva ◽

...

Keyword(s):

Genetic Diversity ◽

Microsatellite Loci

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Genetic diversity and population structure of Bretschneidera sinensis, an endangered species

Biodiversity Science ◽

10.3724/sp.j.1003.2013.09117 ◽

2014 ◽

Vol 21 (6) ◽

pp. 723-731

Author(s):

Xu Gangbiao ◽

Liang Yan ◽

Jiang Yan ◽

Liu Xiongsheng ◽

Hu Shangli ◽

...

Keyword(s):

Genetic Diversity ◽

Population Structure ◽

Endangered Species

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Genetic diversity and genetic structure of the rare and endangered species, Primula ranunculoides

Biodiversity Science ◽

10.3724/sp.j.1003.2013.09098 ◽

2014 ◽

Vol 21 (5) ◽

pp. 601-609

Author(s):

Wang Deyun ◽

Peng Jie ◽

Chen Yajing ◽

Lü Guosheng ◽

Zhang Xiaoping ◽

...

Keyword(s):

Genetic Diversity ◽

Genetic Structure ◽

Endangered Species ◽

Rare And Endangered Species ◽

Rare And Endangered

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Stand out from the Crowd: Small-Scale Genetic Structuring in the Endemic Sicilian Pond Turtle

Diversity ◽

10.3390/d12090343 ◽

2020 ◽

Vol 12 (9) ◽

pp. 343

Author(s):

Luca Vecchioni ◽

Federico Marrone ◽

Marco Arculeo ◽

Uwe Fritz ◽

Melita Vamberger

Keyword(s):

Genetic Diversity ◽

Microsatellite Loci ◽

Small Scale ◽

Moderate Degree ◽

Geographical Pattern ◽

Genetic Structuring ◽

Pond Turtle ◽

Emys Trinacris ◽

Polymorphic Microsatellite ◽

Entire Distribution

The geographical pattern of genetic diversity was investigated in the endemic Sicilian pond turtle Emys trinacris across its entire distribution range, using 16 microsatellite loci. Overall, 245 specimens of E. trinacris were studied, showing high polymorphic microsatellite loci, with allele numbers ranging from 7 to 30. STRUCTURE and GENELAND analyses showed a noteworthy, geographically based structuring of the studied populations in five well-characterized clusters, supported by a moderate degree of genetic diversity (FST values between 0.075 and 0.160). Possible explanations for the genetic fragmentation observed are provided, where both natural and human-mediated habitat fragmentation of the Sicilian wetlands played a major role in this process. Finally, some conservation and management suggestions aimed at preventing the loss of genetic variability of the species are briefly reported, stressing the importance of considering the five detected clusters as independent Management Units.

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