Meander floodplain management and stabilization of riverbank in urban region

The normalization of the Meander River in the past caused environmental problems, changed the river’s ecology, and required heavy maintenance such as stabilizing riverbanks with revetments, sheet piles and other constructions. Pesanggrahan River, located at Southern Region of Jakarta, Indonesia which has a meander channel at the border between the middle reach and downstream reach. The river was normalized at the end of the year 2012 with several modifications such as resection, realignment and cutting-off meandering bends. Analysis the effect of normalization consisting of meander cut-off and riverbank stabilization presented in this paper in term of the behaviour of sediment transport along the channel that has been normalized. HEC-RAS program is used to track the Pesanggrahan river flow from upstream to downstream before and after normalization. The Ackers-White equation used to calculate the amount of sediment for each section based on the change of the river section due to sediment motion after normalizing of the channel river. From the results of the study, it is proposed that a river naturalization or low impact development (LID) approach, such as floodplain management especially at the meander bend with riparian ecosystems that maintain stabilization at the riverbank also maintain habitat heterogeneity, is an important factor for the long-term survival of several rare and endangered species.

Breaking the Limits - Multichromosomal Structure of an Early Eudicot Pulsatilla Patens Mitogenome Reveals Extensive RNA-editing, Longest Repeats and Chloroplast Derived Regions Among Sequenced Land Plant Mitogenomes

Background: The mitogenomes of vascular plants are one of the most structurally diverse molecules. In the present study we characterize mitogenome of a rare and endangered species Pulsatilla patens. We investigated the gene content and its RNA editing potential, repeats distribution and plastid derived sequences.Results: The mitogenome structure of early divergent eudicot, endangered Pulsatilla patens does not support the master chromosome hypothesis, revealing the presence of three linear chromosomes of total length 986 613 bp. The molecules are shaped by the presence of extremely long, exceeding 87 kbp, repeats and multiple chloroplast derived regions including nearly complete inverted repeat. Since the plastid IR content of Ranunculales is very characteristic, the incorporation into mitogenome could be explained rather by intracellular transfer than mitochondrial HGT. The mitogenome contains an almost complete set of genes known from other vascular plants with exception of rps10 and sdh3, the latter being present but pseudogenised. Analysis of long ORFs enabled the identification of genes which are rarely present in plant mitogenomes, including RNA and DNA polymerases, albeit their presence even at species level is variable. Mitochondrial transcripts of P. patens were edited with a high frequency, exceeding the level known in other analyzed angiosperms, despite strict qualification criteria of editing event’s count and analysis of generally less frequently edited leaf transcriptome. The total number of edited sites was 902 and nad4 was identified as the most edited gene with 65 C to U changes. Non-canonical, reverse U to C editing was not detected. Comparative analyses of mitochondrial genes of three Pulsatilla species revealed a level of variation comparable to chloroplast CDS dataset and much higher infrageneric differentiation than in other known angiosperm genera. The variation found in CDS of mitochondrial genes is comparable to values found among Pulsatilla plastomes. Despite a complicated mitogenome structure, 14 single copy regions not splitted by repeats or MTPT of 329 kbp revealed potential for phylogenetic, phylogeographic and population genetics studies by revealing intra- and interspecific collinearity.Conclusions: This studies provides valuable new information about mitochondrial genome of early divergent eudicots, Pulsatilla patens, revealed multi-chromosomal structure and shed new light on mitogenomics of early eudicots.

Climate change and the activities of botanical gardens

Problems and prospects of botanical gardens in a changing climate are discussed in the article. An increase in the average annual surface air temperature, changes in seasonal indicators and the length of the growing season require action from botanical gardens. Decisions on the management of natural and man-made ecosystems, with the aim of their conservation and sustainable development, must be taken taking into account the ecological situation. The knowledge and experience concentrated in botanical gardens makes it possible to assess the threats and opportunities for climate change and develop adaptation measures to them. All activities of botanical gardens should be aimed at counteracting climate change, include preventive measures based on the expected plant responses and compensatory measures. Research work in all areas should take into account the situation with changing climatic parameters. Special attention should be paid to endemic rare and endangered species, historical landscapes, and changes in the assortment of resistant species, cultivation technologies, and biological invasions. Botanical gardens must be proactive by providing information, plant materials and technologies for national and global climate change adaptation programs.

Recent Progress in Spermatology Contributing to the Knowledge and Conservation of Rare and Endangered Species

There is a remarkable diversity in the animal kingdom regarding mechanisms underlying the production, maturation, structure, and function of sperm cells. Spermatology studies contribute to the knowledge of species diversity and also provide information about individual or population fitness. Furthermore, this fundamental research is required before collected spermatozoa can be used for conservation breeding, including assisted reproduction and cryobanking. This article aims to ( a) review the most recent knowledge on sperm morphology and function in wild animal species, ( b) analyze how this knowledge can be used to save species in their natural habitat or ex situ, and ( c) propose future scientific directions in wildlife spermatology that could positively impact animal conservation. Variations in sperm structure and performance within and between species have multiple origins and significance. This collective body of knowledge enables the design and implementation of conservation strategies and action plans that integrate several disciplines. Expected final online publication date for the Annual Review of Animal Biosciences, Volume 10 is February 2022. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

On the conservation of fungi

The article is devoted to the actual problem of conservation of biological diversity in the modern world. The basic principles and specific approaches to the protection of macromycetes as organisms that are difficult in qualitative and quantitative accounting due to the peculiarities of biology and life cycle have been studied. Domestic and global trends and practices in the field of protecting the species diversity of agaricoid fungi as a component of any ecosystem are discussed. The information on the features of regional fungal diversity detection, rare and threatened species for inclusion into the Red lists of various levels as well as on determining the boundaries of fungal populations, including using barcoding methods is provided. It is noted that for the organization of specially protected natural areas, it is necessary to specify and substantiate the changes in the status of separate species, to identify key areas with specific and typical mycological complexes. The idea that the system of mushroom protection should be integrated into the general system of nature protection is substantiated. Along with the protection of rare and endangered fungi species in their typical habitats (nature reserves, nature parks, wildlife areas, etc.), priority directions for preserving the gene pool of fungi in scientific collections and ex situ banks are described. Live mycelial cultures of fungi can be used for the development of new resistant populations by the reintroduction of rare and endangered species, as well as for the production of fungal metabolites useful for humans. There has been also discussed the problem of improving the effectiveness of protective measures through the digitalization of information systems: free and open access to the data on the endangered fungi species through the development of specialized computer databases, websites and unified information banks.

Isoetes baodongii (Isoetaceae), a New Basic Diploid Quillwort from China

Isoetes baodongii Y. F. Gu, Y. H. Yan & Yi J. Lu (Isoetaceae) is here described as a newly discovered, rare and endangered species of quillwort from eastern China. It has a basic diploid chromosome count of 2n = 22. Isoetes baodongii is similar to I. sinensis T. C. Palmer in its overall appearance and was previously misidentified as such. It differs from I. sinensis in its chromosome number and in megaspore ornamentation. In I. baodongii, both the proximal surface and distal surface of the megaspore are echinato-cristate, whereas in I. sinensis the proximal surface is echinate and the distal surface echinato-cristate. Microspores of I. baodongii have echinate ornamentation.

Dynamic Transcriptomic and Metabolomic Analyses of Madhuca pasquieri (Dubard) H. J. Lam During the Post-germination Stages

The wild population of Madhuca pasquieri (Dubard) H. J. Lam is currently dwindling; its understory seedlings are rare, and there is a lack of molecular studies, which impedes the conservation of this species. This study exploited second-generation sequencing and widely targeted metabolomics analysis to uncover the dynamic changes in differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) in five post-germination stages of M. pasquieri whole organism. Notably, the weighted gene co-expression network analysis (WGCNA), transcriptome, and metabolome association analyses all indicated significant enrichment of the flavonoid biosynthesis pathway in stage 4 (two-leaf), and an upregulation of the genes encoding flavonol biosynthesis in this stage. In stage 5 (nine-leaf), the flavonols were significantly accumulated, indicating that the changes in metabolites were driven at the transcript level. According to the significant changes in gene expression encoding auxin transport carriers and their correlation with flavonols during stage 5, the flavonols were speculated to have a direct inhibitory effect on the expression of PIN4 encoding gene, which may inhibit the process of polar auxin transport. The results provided important insights into the molecular network relationships between the transcription and metabolism of this rare and endangered species during the post-germination stages and explained the reasons for the slow growth of its seedlings at the molecular level.

Features of the water regime of aboriginal and introduced species of the genus Iris L. in the Southern Ural

The article presents the results of an experimental assessment of the peculiarities of the water regime of 9 species of the genus Iris L. growing in the South-Ural Botanical Garden-Institute UFRC RAS (I. sibirica L., I. pseudacorus L. ‒ species of native flora, I. aphylla L., I. biglumis Vahl., I. lacteal Pall., I. orientalis Mill., I. ruthenica Ker-Gawl., I. setosa Pall., I. spuria L. ‒ introduced species). The purpose is a comparative evaluation of the main parameters of the water regime within the generic complex and depending on the detection of meteorological factors in different phenological periods. Methods. Studies were performed in growing periods 2019–2020’s physiological using conventional techniques (artificial saturation method and wilting). Made a detailed analysis of daily and seasonal dynamics of the water regime of the three parameters: the total water content, water-holding capacity, water scarcity. Typical forest species Convallaria majalis L. was investigated for a comparative analysis of water regime indicators. Results. The similarities and differences in the peculiarities of the water regime were established between the studied species, the dependence of the indicators on meteorological conditions was revealed. According to the type of water regime cultivars were divided into four groups: a flexible water-quiet mode ‒ I. pseudacorus, flexible water-tight mode ‒ I. sibirica, I. ruthenica, stably-calm water mode ‒ I. aphylla, I. biglumis, I. setosa, stably-tight water mode ‒ I. spuria, I. lactea, I. orientalis. Among the studied parameters of water scarcity was the most dependent on meteorological factors. Scientific novelty. The study helps to identify ecological and physiological adaptations of exotic species in comparison with the native species that could become the basis for assessing the prospects of growing in the culture and conservation of rare and endangered species.

Some rare Heteroptera species (Hemiptera) from the „Cobîleni” natural reserve, Republic of Moldova

A list of the some rare heteroptera species from the „Cobîleni” Natural Reserve is published. The list contains 9 species from 5 families: Corixidae (3 species), Anthocoridae (1), Miridae (3), Lygaeidae (1) and Pentatomidae – 1 species. Data on bio-ecology and host plants are given. It is noted that the „Cobîleni” Reserve are the guarantor of the preservation of both typical biotopes and rare and endangered species of true bugs.