scholarly journals Evaluation of the MB/BacT system compared with Middlebrook 7H11 and Lowenstein-Jensen media for detection and recovery of mycobacteria from clinical specimens

2004 ◽  
Vol 10 (3) ◽  
pp. 224-228 ◽  
Author(s):  
A. Gil-Setas ◽  
L. Torroba ◽  
J.L. Fernandez ◽  
V. Martinez-Artola ◽  
J. Olite
Keyword(s):  
Clinical Specimens ◽  
Lowenstein Jensen

scholarly journals Multicenter Comparison of ESP Culture System II with BACTEC 460TB and with Lowenstein-Jensen Medium for Recovery of Mycobacteria from Different Clinical Specimens, Including Blood

1998 ◽  
Vol 36 (5) ◽  
pp. 1378-1381 ◽  
Author(s):  
Enrico Tortoli ◽  
Paola Cichero ◽  
M. Gabriella Chirillo ◽  
M. Rita Gismondo ◽  
Letizia Bono ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Avium ◽  
Culture System ◽  
Solid Medium ◽  
Mycobacterial Species ◽  
Becton Dickinson ◽  
Clinical Specimens ◽  
Lowenstein Jensen ◽  
Bactec System ◽  
The Times

The recently developed ESP Culture System II (AccuMed, Chicago, Ill.) was compared with radiometric BACTEC 460TB (Becton Dickinson, Towson, Md.) and with Lowenstein-Jensen medium for recovery of mycobacteria from over 2,500 clinical specimens both of respiratory and nonrespiratory origin, including blood. The majority of the 219 mycobacterial isolates (129) belonged to the Mycobacterium tuberculosis complex, followed by 37 isolates of theMycobacterium avium complex (MAC) and 53 isolates of eight other mycobacterial species. Rates of recovery obtained with BACTEC, ESP, and Lowenstein-Jensen medium were 89, 79, and 64%, respectively, with such differences being statistically significant. Different media and systems appeared to behave differently when the more frequently detected organisms were considered: M. tuberculosis complex isolates grew better with BACTEC, and MAC isolates grew better with ESP. An analysis of the combinations of Lowenstein-Jensen medium with BACTEC and with ESP did not reveal significant differences in recovery rates. With regard to the times needed for the detection of positive cultures, they were significantly longer on Lowenstein-Jensen medium (average, 28 days) than with the remaining two systems, between which there was no difference (average, 18 days). We conclude, therefore, that the ESP system, when used in combination with a solid medium, performs as well as the thoroughly validated radiometric BACTEC system and offers the advantages of full automation and absence of radioisotopes.

Comparison of the VersaTREK system and Löwenstein-Jensen medium for the recovery of mycobacteria from clinical specimens

2008 ◽  
Vol 40 (1) ◽  
pp. 49-53 ◽  
Author(s):  
Francisco Quiñones Falconi ◽  
Lourdes Infante Suárez ◽  
Manuel de jesús castillejos López ◽  
Cecilia garcía Sancho
Keyword(s):  
Clinical Specimens ◽  
Lowenstein Jensen

scholarly journals A Novel Solid Medium for Culturing Mycobacterium tuberculosis Isolates from Clinical Specimens

2015 ◽  
Vol 53 (8) ◽  
pp. 2566-2569 ◽  
Author(s):  
S. Asmar ◽  
S. Chatellier ◽  
C. Mirande ◽  
A. van Belkum ◽  
I. Canard ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Solid Medium ◽  
Laboratory Diagnosis ◽  
Contamination Rate ◽  
Content Type ◽  
Exact Test ◽  
Clinical Specimens ◽  
Fisher's Exact Test ◽  
Fisher’S Exact Test ◽  
Lowenstein Jensen

The laboratory diagnosis of tuberculosis usually relies on culture-based isolation of the causativeMycobacterium tuberculosisbacteria. We developed and evaluated the performance of MOD9, a new blood-free derivative of the MOD4 solid medium on which we previously reported for the isolation and culture of mycobacteria. First, inoculation of Lowenstein-Jensen medium with 21M. tuberculosisisolates at 105, 103, and 10 CFU yielded colonies in 5.7 ± 1.5 days, 7.6 ± 0.8 days, and 10.8 ± 1.7 days versus 1.5 ± 0.4 days, 3.5 ± 0.6 days, and 4.9 ± 1 days in MOD9 (P< 0.05, Student'sttest). Further, the time to detectable growth ofM. tuberculosiswas measured on MOD9 and Lowenstein-Jensen media after duplicate inoculation of 250 clinical specimens decontaminated with 0.7% chlorhexidine. The contamination rate was 1.6% (4/250) on MOD9 versus 4.4% (11/250) on Lowenstein-Jensen medium (P= 0.11, Fisher's exact test). Chlorhexidine-MOD9 yielded 38/250 (15.2%) isolates versus 32/250 (12.8%) isolates for the chlorhexidine-LJ (P= 0.5195, Fisher's exact test). All together, eightM. tuberculosisisolates were cultured solely from chlorhexidine-MOD9, and twoM. tuberculosisisolates were cultured solely from chlorhexidine-LJ. The time to detection was 9.8 ± 3.9 (range, 5 to 18) days for chlorhexidine-MOD9 versus 17.4 ± 5.9 (range, 10 to 35) days for chlorhexidine-LJ (P< 0.05, Student'sttest). These data indicate the superiority of the MOD9 medium over the standard LJ medium following chlorhexidine decontamination for the recovery ofM. tuberculosis.

scholarly journals Fast, Simple, and Cheap: the Kudoh-Ogawa Swab Method as an Alternative to the Petroff–Lowenstein-Jensen Method for Culturing of Mycobacterium tuberculosis

2020 ◽  
Vol 58 (4) ◽  
Author(s):  
Greta Franco-Sotomayor ◽  
Ismar A. Rivera-Olivero ◽  
Margarita Leon-Benitez ◽  
Sandra E. Uruchima-Campoverde ◽  
Greta Cardenas-Franco ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Diagnostic Yield ◽  
Turnaround Time ◽  
Clinical Samples ◽  
Content Type ◽  
Clinical Specimens ◽  
Significant Difference ◽  
High Workload ◽  
Lowenstein Jensen ◽  
Culture Growth

ABSTRACT The objective of this study was to compare the diagnostic yield of the Kudoh-Ogawa (K-O) swab method for the culturing of Mycobacterium tuberculosis from clinical samples with the standard Petroff–Lowenstein-Jensen (P-LJ) procedure. A total of 2,287 sputum samples and 685 extrapulmonary clinical specimens were processed with both decontamination methods and compared for M. tuberculosis detection rate, recovery of M. tuberculosis colonies, and culture contamination. Overall, 23.9% and 23.5% of the samples, processed with, respectively, the K-O swab method and the P-LJ procedure, yielded M. tuberculosis after 8 weeks of incubation. The K-O swab method and the P-LJ procedure provided comparable diagnostic yields for extrapulmonary clinical specimens (P = 0.688), but the K-O method showed a slightly but statistically significantly higher diagnostic yield for pulmonary samples (P = 0.002). No significant difference for culture contamination or colony recovery was found for either method. The turnaround time for the isolation of M. tuberculosis was significantly shorter for the K-O swab method, with 77% of the M. tuberculosis cultures being positive within 3 weeks of incubation, and only 6.1% positivity for the P-LJ method. Concerning the workload, the K-O swab method needs a minimum sample manipulation and takes less than 4 min per sample, as the samples are not centrifuged in this procedure. The K-O swab method is an efficient and fast (in terms of sample processing and culture growth) alternative for culturing M. tuberculosis from either pulmonary or extrapulmonary clinical specimens. The method is particularly suitable for laboratories with a high workload and for laboratories lacking a special infrastructure.

Comparison of a biphasic non-radiometric system with Lowenstein-Jensen and Bactec-460 system for recovery of mycobacteria from clinical specimens

1996 ◽  
Vol 77 (5) ◽  
pp. 449-453 ◽  
Author(s):  
M. Luquin ◽  
F. Gamboa ◽  
M.García Barceló ◽  
J.M. Manterola ◽  
L. Matas ◽  
...  
Keyword(s):  
Clinical Specimens ◽  
Radiometric System ◽  
Lowenstein Jensen

scholarly journals Comparison of recoveries of Mycobacterium tuberculosis using the automated BACTEC MGIT 960 System and Lowenstein – Jensen medium

2010 ◽  
Vol 9 (1) ◽  
pp. 44
Author(s):  
Mo. A.AL-Mazini, T. Bukeet, and A. Abdul Kareem
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Recovery Rates ◽  
Clinical Specimens ◽  
Indicator Tube ◽  
Lowenstein Jensen ◽  
Bactec System ◽  
Smear Negative ◽  
The Difference ◽  
Growth Indicator

We examined whether the BACTEC/ Mycobacteria Growth Indicator Tube (MGIT) System alone could supplant the use of a supplemental Lowenstein–Jensen (LJ) slant for routine recovery of M. tuberculosis from clinical specimens. A total of 392 specimens of sputum were included in the study, collected from 196 patients. Specimens were processed with standard N-acetyl- L- Cysteine (NALC-NaOH) method, then inoculated onto BACTEC MGIT 960 and onto LJ media. The recovery rates of M.tuberculosis were 100 % (256/256) with BACTEC MGIT 960 and 72.6%(186/256) with LJ. The rates of contamination for each of the system were 4.8%with BACTEC MGIT 960 and 5.3%with LJ. The TTD for M. tuberculosis was 11.3 days with BACTEC System and 30.8 days with LJ. The difference in TTD between smear positive and smear negative specimens for M.tuberculosis with BACTEC MGIT 960 was not statistically significant. This study shows that the BACTEC System demonstrates better sensitivity for the recovery of M. tuberculosis from clinical specimens.

scholarly journals Comparison of the MB/BacT and BACTEC 460 TB Systems for Recovery of Mycobacteria from Various Clinical Specimens

1999 ◽  
Vol 37 (4) ◽  
pp. 1206-1209 ◽  
Author(s):  
Francesca Brunello ◽  
Flavio Favari ◽  
Roberta Fontana
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Nontuberculous Mycobacteria ◽  
Common Species ◽  
Individual Species ◽  
Mycobacterium Chelonae ◽  
Recovery Rates ◽  
Clinical Specimens ◽  
Lowenstein Jensen ◽  
Smear Negative ◽  
The Difference

A total of 1,830 specimens (75.7% respiratory and 24.3% nonrespiratory) were cultured in parallel with the MB/BacT and BACTEC 460 TB systems and on Lowenstein-Jensen (LJ) medium. Mycobacteria were identified from 173 (6.5%) specimens. The most common species recovered were Mycobacterium tuberculosis complex (65.9%),Mycobacterium avium complex (22.5%), andMycobacterium chelonae (9.2%). The recovery rates by individual systems were 96.5, 99.4, and 95.9% for MB/BacT, BACTEC 460 TB, and LJ medium, respectively, for all mycobacteria; the recovery rates were 99.1, 100, and 98.2%, respectively, for M. tuberculosis complex alone. The difference among the recovery rates for all mycobacteria and those for individual species was not significant. The BACTEC 460 TB system detected M. tuberculosis isolates more rapidly than the MB/BacT system (8 versus 11.8 days for smear-positive specimens [P < 0.01] and 18 versus 21 days for smear-negative specimens [P < 0.05]), whereas the MB/BacT system more rapidly detected the nontuberculous mycobacteria (17.1 versus 12.7 days [P < 0.01]). These results indicate that the nonradiometric MB/BacT system is a rapid, sensitive, and efficient method for the recovery ofM. tuberculosis and nontuberculous mycobacteria from both pulmonary and extrapulmonary clinical specimens.

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