scholarly journals T-cell receptor binding affinities and kinetics: impact on T-cell activity and specificity

Immunology ◽  
2009 ◽  
Vol 126 (2) ◽  
pp. 165-176 ◽  
Author(s):  
Jennifer D. Stone ◽  
Adam S. Chervin ◽  
David M. Kranz
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Receptor Binding ◽  
Cell Activity ◽  
Cell Receptor ◽  
Binding Affinities

scholarly journals Methods for Quantifying T cell Receptor Binding Affinities and Thermodynamics

2009 ◽  
pp. 359-381 ◽  
Author(s):  
Kurt H. Piepenbrink ◽  
Brian E. Gloor ◽  
Kathryn M. Armstrong ◽  
Brian M. Baker
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Receptor Binding ◽  
Cell Receptor ◽  
Binding Affinities

scholarly journals Characterization of human T cells reactive with the Mycoplasma arthritidis-derived superantigen (MAM): generation of a monoclonal antibody against V beta 17, the T cell receptor gene product expressed by a large fraction of MAM-reactive human T cells.

1991 ◽  
Vol 174 (4) ◽  
pp. 891-900 ◽  
Author(s):  
S M Friedman ◽  
M K Crow ◽  
J R Tumang ◽  
M Tumang ◽  
Y Q Xu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
T Cells ◽  
T Cell ◽  
T Cell Receptor ◽  
Cell Activity ◽  
Cell Receptor ◽  
Cytotoxic T Cell ◽  
Mycoplasma Arthritidis ◽  
Human T Cells

While all known microbial superantigens are mitogenic for human peripheral blood lymphocytes (PBL), the functional response induced by Mycoplasma arthritidis-derived superantigen (MAM) is unique in that MAM stimulation of PBL consistently results in T cell-dependent B cell activation characterized by polyclonal IgM and IgG production. These immunostimulatory effects of MAM on the humoral arm of the human immune system warranted a more precise characterization of MAM-reactive human T cells. Using an uncloned MAM reactive human T cell line as immunogen, we have generated a monoclonal antibody (mAb) (termed C1) specific for the T cell receptor V beta gene expressed by the major fraction of MAM-reactive human T cells, V beta 17. In addition, a V beta 17- MAM-reactive T cell population exists, assessed by MAM, induced T cell proliferation and cytotoxic T cell activity. mAb C1 will be useful in characterizing the functional properties of V beta 17+ T cells and their potential role in autoimmune disease.

scholarly journals Single MHC Mutation Eliminates Enthalpy Associated with T Cell Receptor Binding

2007 ◽  
Vol 373 (2) ◽  
pp. 315-327 ◽  
Author(s):  
Peter J. Miller ◽  
Yael Pazy ◽  
Brian Conti ◽  
David Riddle ◽  
Ettore Appella ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Receptor Binding ◽  
Cell Receptor

scholarly journals Crystallographic and Mutational Data Show That the Streptococcal Pyrogenic Exotoxin J Can Use a Common Binding Surface for T-cell Receptor Binding and Dimerization

2004 ◽  
Vol 279 (37) ◽  
pp. 38571-38576 ◽  
Author(s):  
Heather M. Baker ◽  
Thomas Proft ◽  
Phillip D. Webb ◽  
Vickery L. Arcus ◽  
John D. Fraser ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Receptor Binding ◽  
Cell Receptor ◽  
Binding Surface

scholarly journals T Cell Receptor Binding to a pMHCII Ligand Is Kinetically Distinct from and Independent of CD4

2000 ◽  
Vol 276 (8) ◽  
pp. 5659-5667 ◽  
Author(s):  
Yi Xiong ◽  
Petra Kern ◽  
Hsiu-Ching Chang ◽  
Ellis L. Reinherz
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Receptor Binding ◽  
Cell Receptor

scholarly journals Qualitative and Quantitative Differences in T Cell Receptor Binding of Agonist and Antagonist Ligands

Immunity ◽  
1999 ◽  
Vol 10 (2) ◽  
pp. 227-237 ◽  
Author(s):  
S.Munir Alam ◽  
G.Mark Davies ◽  
Christina M. Lin ◽  
Tomasz Zal ◽  
Wade Nasholds ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Receptor Binding ◽  
Cell Receptor ◽  
Qualitative And Quantitative ◽  
Agonist And Antagonist

scholarly journals Geometrical characterization of T cell receptor binding modes reveals class‐specific binding to maximize access to antigen

2019 ◽  
Vol 88 (3) ◽  
pp. 503-513 ◽  
Author(s):  
Nishant K. Singh ◽  
Esam T. Abualrous ◽  
Cory M. Ayres ◽  
Frank Noé ◽  
Ragul Gowthaman ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Receptor Binding ◽  
Specific Binding ◽  
Cell Receptor ◽  
Binding Modes ◽  
Geometrical Characterization

scholarly journals Cd8− T Cell Transfectants That Express a High Affinity T Cell Receptor Exhibit Enhanced Peptide-Dependent Activation

2001 ◽  
Vol 194 (8) ◽  
pp. 1043-1052 ◽  
Author(s):  
Phillip D. Holler ◽  
Alice R. Lim ◽  
Bryan K. Cho ◽  
Laurie A. Rund ◽  
David M. Kranz
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Receptor ◽  
Cytotoxic T Lymphocyte ◽  
Cell Activity ◽  
Cell Receptor ◽  
Wild Type ◽  
High Affinity ◽  
Antigen Presenting

T cells are activated by binding of the T cell receptor (TCR) to a peptide-major histocompatibility complex (MHC) complex (pMHC) expressed on the surface of antigen presenting cells. Various models have predicted that activation is limited to a narrow window of affinities (or dissociation rates) for the TCR–pMHC interaction and that above or below this window, T cells will fail to undergo activation. However, to date there have not been TCRs with sufficiently high affinities in order to test this hypothesis. In this report we examined the activity of a CD8-negative T cell line transfected with a high affinity mutant TCR (KD = 10 nM) derived from cytotoxic T lymphocyte clone 2C by in vitro engineering. The results show that despite a 300-fold higher affinity and a 45-fold longer off-rate compared with the wild-type TCR, T cells that expressed the mutant TCRs were activated by peptide. In fact, activation could be detected at significantly lower peptide concentrations than with T cells that expressed the wild-type TCR. Furthermore, binding and functional analyses of a panel of peptide variants suggested that pMHC stability could account for apparent discrepancies between TCR affinity and T cell activity observed in several prior studies.

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