Molecular characterization of a strain-specific repeated DNA sequence in the fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae)

Abstract The fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith) is a polyphagous Lepidopteran pest, a native to tropical and sub-tropical America and recently it has invaded the African and Asian countries. Presently, the mitochondrial Cytochrome Oxidase Subunit I (COI) - gene based molecular characterization of FAW samples from the maize fields of southern Rajasthan has revealed the occurrence of corn and rice strains there. The occurrence of such S. frugiperda population of Rajasthan region could be traced its origin from the Florida-Caribbean region or African region. Further, the Tpi gene region analysis showed that the S.frugiperda forms found in the maize fields are only the corn strains. In the Indian Rajasthan populations of FAW, the Tpi-variant2 category is the highest one and is then followed by the Tpi-variant1 and Tpi-variant3 was unique with C and T at Tpie4192 and Tpie4198, respectively. Further research is needed towards the confirmation of these tentatively identified strains of S. frugiperda that would in turn helpful for the proper monitoring, host-plant identification and the effective management of such pests.

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Cytogenetic and molecular characterization of a highly repeated DNA sequence in the peach potato aphid Myzus persicae

Chromosoma ◽

10.1007/s004120050395 ◽

1999 ◽

Vol 108 (7) ◽

pp. 436-442 ◽

Cited By ~ 40

Author(s):

M. Mandrioli ◽

D. Bizzaro ◽

G. C. Manicardi ◽

D. Gionghi ◽

L. Bassoli ◽

...

Keyword(s):

Molecular Characterization ◽

Dna Sequence ◽

Myzus Persicae ◽

Repeated Dna ◽

Potato Aphid ◽

Highly Repeated Dna ◽

Repeated Dna Sequence ◽

Peach Potato Aphid

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Characterization of a major tandemly repeated DNA sequence (RBMII) prevalent among many species of waterfowl (Anatidae)

Genome ◽

10.1139/g92-159 ◽

1992 ◽

Vol 35 (6) ◽

pp. 1037-1044 ◽

Cited By ~ 4

Author(s):

Cort S. Madsen ◽

Kevin P. McHugh ◽

Siwo R. de Kloet

Keyword(s):

Dna Sequence ◽

Sequence Variation ◽

Sequence Divergence ◽

Repeated Dna ◽

Sequence Comparisons ◽

Nonrandom Distribution ◽

Repeated Dna Sequence ◽

Tandemly Repeated Dna Sequence ◽

Tandemly Repeated Dna

We have investigated the evolution of a 190 base pair tandemly repeated DNA sequence (RBMII) in 27 different species of waterfowl. In this paper we show that the RBMII sequence is present in many species belonging to 7 of the 11 Anatid tribes. Inter- and intra-tribal differences in repeat presence indicate that, although the RBMII sequence has been maintained among widely divergent species, it is rapidly evolving. Restriction enzyme analyses suggest very different hierarchical repeat organizations among different species. DNA sequence comparisons of 32 cloned monomer units from five different species revealed what appears to be a nonrandom distribution of sequence divergence, as well as large differences (up to 25-fold) in intraspecific sequence variation between relatively closely related species.Key words: repeated DNA, Anatidae, sequence variation.

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Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2008000400010 ◽

2008 ◽

Vol 43 (4) ◽

pp. 513-520 ◽

Cited By ~ 11

Author(s):

Andréa Almeida Carneiro ◽

Eliane Aparecida Gomes ◽

Claudia Teixeira Guimarães ◽

Fernando Tavares Fernandes ◽

Newton Portilho Carneiro ◽

...

Keyword(s):

Molecular Characterization ◽

Spodoptera Frugiperda ◽

Sequence Comparison ◽

Rapd Markers ◽

Fall Armyworm ◽

Its Sequence ◽

Its Sequencing ◽

Host Insect ◽

Rdna Its

The objective of this work was to evaluate the pathogenicity of 24 Beauveria isolates to Spodoptera frugiperda larvae, and characterize them molecularly through rDNA-ITS sequencing and RAPD markers. Sequencing of rDNA-ITS fragments of 570 bp allowed the identification of isolates as B. bassiana or B. brongniarti by sequence comparison to GenBank. Sixty seven polymorphic RAPD fragments were capable to differentiate 20 among 24 Beauveria isolates, grouping them according to the derived host insect and to pathogenicity against maize fall armyworm larvae. Three RAPD markers were highly associated to the pathogenicity against S. frugiperda, explaining up to 67% of the phenotypic variation. Besides identification and molecular characterization of Beauveria isolates, ITS sequence and RAPD markers proved to be very useful in selecting the isolates potentially effective against S. frugiperda larvae and in monitoring field release of these microorganisms in biocontrol programs.

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