Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm

The objective of this work was to evaluate the pathogenicity of 24 Beauveria isolates to Spodoptera frugiperda larvae, and characterize them molecularly through rDNA-ITS sequencing and RAPD markers. Sequencing of rDNA-ITS fragments of 570 bp allowed the identification of isolates as B. bassiana or B. brongniarti by sequence comparison to GenBank. Sixty seven polymorphic RAPD fragments were capable to differentiate 20 among 24 Beauveria isolates, grouping them according to the derived host insect and to pathogenicity against maize fall armyworm larvae. Three RAPD markers were highly associated to the pathogenicity against S. frugiperda, explaining up to 67% of the phenotypic variation. Besides identification and molecular characterization of Beauveria isolates, ITS sequence and RAPD markers proved to be very useful in selecting the isolates potentially effective against S. frugiperda larvae and in monitoring field release of these microorganisms in biocontrol programs.

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Molecular characterization of a strain-specific repeated DNA sequence in the fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae)

Insect Molecular Biology ◽

10.1111/j.1365-2583.1994.tb00159.x ◽

1994 ◽

Vol 3 (2) ◽

pp. 123-130 ◽

Cited By ~ 44

Author(s):

Y.-J. Lu ◽

G. D. Kochert ◽

D. J. Isenhour ◽

M. J. Adang

Keyword(s):

Molecular Characterization ◽

Dna Sequence ◽

Spodoptera Frugiperda ◽

Fall Armyworm ◽

Repeated Dna ◽

Repeated Dna Sequence ◽

Lepidoptera Noctuidae

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Molecular characterization of the Fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) on maize in southern Rajasthan, India

10.21203/rs.3.rs-652999/v1 ◽

2021 ◽

Author(s):

Sakthivel Ramesh Babu ◽

Perumal Pachippan ◽

Raja Manoharan ◽

Sonika Joshi ◽

Deepika Kalyan ◽

...

Keyword(s):

Molecular Characterization ◽

Spodoptera Frugiperda ◽

Fall Armyworm ◽

Caribbean Region ◽

Plant Identification ◽

African Region ◽

Tropical America ◽

Lepidopteran Pest ◽

Lepidoptera Noctuidae

Abstract The fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith) is a polyphagous Lepidopteran pest, a native to tropical and sub-tropical America and recently it has invaded the African and Asian countries. Presently, the mitochondrial Cytochrome Oxidase Subunit I (COI) - gene based molecular characterization of FAW samples from the maize fields of southern Rajasthan has revealed the occurrence of corn and rice strains there. The occurrence of such S. frugiperda population of Rajasthan region could be traced its origin from the Florida-Caribbean region or African region. Further, the Tpi gene region analysis showed that the S.frugiperda forms found in the maize fields are only the corn strains. In the Indian Rajasthan populations of FAW, the Tpi-variant2 category is the highest one and is then followed by the Tpi-variant1 and Tpi-variant3 was unique with C and T at Tpie4192 and Tpie4198, respectively. Further research is needed towards the confirmation of these tentatively identified strains of S. frugiperda that would in turn helpful for the proper monitoring, host-plant identification and the effective management of such pests.

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Molecular Characterization of the Bacillus thuringiensis (Berliner) Strains 344 and 1644, Efficient Against Fall Armyworm Spodoptera frugiperda (J. E. Smith)

Revista Brasileira de Milho e Sorgo ◽

10.18512/1980-6477/rbms.v7n3p195-209 ◽

2008 ◽

Vol 7 (3) ◽

pp. 195-209 ◽

Cited By ~ 3

Author(s):

F.H. Valicente ◽

U.G.P. Lana

Keyword(s):

Bacillus Thuringiensis ◽

Molecular Characterization ◽

Spodoptera Frugiperda ◽

Fall Armyworm

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Molecular characterization of Cry1F resistance in fall armyworm, Spodoptera frugiperda from Brazil

Insect Biochemistry and Molecular Biology ◽

10.1016/j.ibmb.2019.103280 ◽

2020 ◽

Vol 116 ◽

pp. 103280 ◽

Cited By ~ 14

Author(s):

Debora Boaventura ◽

Julia Ulrich ◽

Bettina Lueke ◽

Anderson Bolzan ◽

Daniela Okuma ◽

...

Keyword(s):

Molecular Characterization ◽

Spodoptera Frugiperda ◽

Fall Armyworm

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Molecular Characterization of Bacillus thuringiensis cyt Genes and Their Effect Against Fall Armyworm, Spodoptera frugiperda

Journal of Agricultural Science ◽

10.5539/jas.v6n7p128 ◽

2014 ◽

Vol 6 (7) ◽

Author(s):

Maria L. M. Costa ◽

Ubiraci G. P. Lana ◽

Emerson C. Barros ◽

Luciano V. Paiva ◽

Fernando H. Valicente

Keyword(s):

Bacillus Thuringiensis ◽

Molecular Characterization ◽

Spodoptera Frugiperda ◽

Fall Armyworm

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Molecular characterization of fall armyworm (Spodoptera frugiperda) resistant to Vip3Aa20 protein expressed in corn

10.11606/t.11.2017.tde-24082017-185006 ◽

2017 ◽

Author(s):

Júlio César Fatoretto

Keyword(s):

Molecular Characterization ◽

Spodoptera Frugiperda ◽

Fall Armyworm

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Virulence and Genetic Characterization of Six Baculovirus Strains Isolated From Different Populations of Spodoptera Frugiperda (Lepidoptera: Noctuidae)

10.21203/rs.3.rs-988050/v1 ◽

2021 ◽

Author(s):

Ingrid Zanella-Saenz ◽

Elisabeth A. Herniou ◽

Jorge E. Ibarra ◽

Ma.Cristina Del Rincón-Castro ◽

Ilse Alejandra Huerta-Arredondo

Keyword(s):

Biological Control ◽

Cell Cultures ◽

Spodoptera Frugiperda ◽

Fall Armyworm ◽

The United States ◽

Infection Mechanisms ◽

Different Populations

Abstract Fall armyworm (FAW), Spodoptera frugiperda (Smith, 1797), is a polyphagous, voracious, and economically important agricultural pest. Biological control of FAW is a strategy that must be further explored. This study evaluated six baculovirus strains isolated from infected FAW larvae from Mexico, Argentina, Honduras, and the United States. Five alphabaculoviruses (SfNPV-An2, SfNPV-Arg, SfNPV-Fx, SfNPV-Ho and SfNPV-Sin) and one betabaculovirus (SfGV-RV), were tested against FAW larvae, showing a wide diversity of virulence levels among strains when their estimated LC50s were compared, being SfNPVArg, SfNPV-Ho and SfNPV-Fx more virulent than SfNPV-An 2 , SfNPV-Sin and SfGV-RV. To determine any virulence difference in vitro studies of these isolates, Sf9 cell cultures were used. Interestingly, only ODVs from four of the test SfNPV strains showed infectivity on Sf9 cell cultures, and some differences in virulence were observed. Genomic restriction analyses and partial sequences of lef-8, lef-9 , and polh/granulin genes showed little variability among alphabaculoviruses, both, among them and with previously reported sequences. However, sequences from SfGV-RV were closer to previously reported sequences from the SfGVVG008 strain than the SfGV-Arg and SfGV-VG014 strains. The great difference in the in vivo virulence was not correlated with great similarity among the isolates. The characterization of these six baculoviruses isolates offers the basis for exploring their potential as biological control agents against S. frugiperda, as well the initial studies on their specific infection mechanisms, evolution, and ecology.

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