Random amplification of polymorphic DNA (RAPD) of Salmonella: strain differentiation and characterization of amplified sequences

1996 ◽  
Vol 81 (6) ◽  
pp. 575-584 ◽  
Author(s):  
A.C. Hilton ◽  
J.G. Banks ◽  
C.W. Penn
Keyword(s):  
Strain Differentiation ◽  
Random Amplification

scholarly journals Random Amplification of Polymorphic DNA Analysis for Genetic Characterization of Two Breeds of Dogs, German Shepherd and Japanese Spitz

2013 ◽  
Vol 13 (2) ◽  
pp. 73-78
Author(s):  
Jarina Joshsi ◽  
Lumanti Manandhar ◽  
Patima Shrestha ◽  
Rani Gupta ◽  
Rojlina Manadhar ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Dna Analysis ◽  
Genetic Characterization ◽  
Random Amplified Polymorphic Dna ◽  
Neighbor Joining ◽  
German Shepherd ◽  
Polymorphic Loci ◽  
Random Amplification

Random amplified polymorphic DNA (RAPD) markers were used to study genetic diversity in dog samples belonging to populations of German Shepherd and Japanese Spitz. A total of twelve samples were typed using eight RAPD primers. Out of eight primers, three primers gave result in six individuals of dogs. The phylogenetic tree constructed by the neighbor joining method based on Nei. Original measures revealed highest genetic identity found in German Shepherd as 0.9444 and highest genetic distance as 1.2809. The analysis predicts the number of polymorphic loci as 15 and the percentage of polymorphic loci as 83.3. Nepal Journal of Science and Technology Vol. 13, No. 2 (2012) 73-78 DOI: http://dx.doi.org/10.3126/njst.v13i2.7717

scholarly journals Molecular characterization of Streptococcus mutans gtfB gene isolated from families

2018 ◽  
Vol 33 (1) ◽  
pp. 40
Author(s):  
Monica Moreira ◽  
Mariana Machado Fidelis do Nascimento ◽  
Mariane Moreira Poletto ◽  
Débora Do Rocio Kliisiowicz ◽  
Renata Rodrigues Gomes ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Dental Enamel ◽  
Extracellular Polysaccharides ◽  
Rrna Gene ◽  
Partial Sequencing ◽  
Dental Biofilm ◽  
Potential Marker ◽  
Random Amplification ◽  
Genetic Pattern

OBJECTIVE: Caries is a multifactorial infectious disease and the main etiological agent is the bacteria Streptococcus mutans due to its virulence factors, which enable the adherence to dental enamel and favours the formation of dental biofilm through the production of extracellular polysaccharides. The transmission of S. mutans can occur between people, often within families. The present study aimed to evaluate the intrafamily genetic pattern of S. mutans through partial sequencing of the gene that encodes the glucosyltransferase β (gtfB) enzyme.METHODS: We previously analyzed saliva samples from 40 individuals from nine families, and it was obtained 64 isolates biochemically characterized as S. mutans. The isolates were evaluated by random amplification of polymorphic DNA (RAPD). Those with greater similarity were characterized by partial sequence 16S rRNA gene and the gtfB gene sequencing.RESULTS: It was observed genetic similarity among strains isolated from individuals with caries activity; while isolates from individual without caries showed that they are genetically distinct, suggesting a different virulence pattern.CONCLUSION: The present results demonstrated that partial sequencing of the gtfB gene showed to be a potential marker to investigate genetic pattern and virulence of S. mutans, deserving further investigation in order to identify families at risk of caries.

Genotypic Characterization of Avian Escherichia coli by Random Amplification of Polymorphic DNA

2005 ◽  
Vol 4 (6) ◽  
pp. 378-381 ◽  
Author(s):  
Amitha R. Gomes . ◽  
L. Muniyappa . ◽  
G. Krishnappa . ◽  
V.V.S. Suryanarayana . ◽  
S. Isloor . ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genotypic Characterization ◽  
Random Amplification

Characterization of enterotoxigenic Escherichia coli by random amplification of polymorphic DNA

1996 ◽  
Vol 147 (3) ◽  
pp. 175-182 ◽  
Author(s):  
A.B.F Pacheco ◽  
B.E.C Guth ◽  
D.F de Almeida ◽  
L.C.S Ferreira
Keyword(s):  
Escherichia Coli ◽  
Enterotoxigenic Escherichia Coli ◽  
Random Amplification
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