Brachygnathia Inferior in Cloned Dogs Is Possibly Correlated with Variants of Wnt Signaling Pathway Initiators

Abnormalities in animals cloned via somatic cell nuclear transfer (SCNT) have been reported. In this study, to produce bomb-sniffing dogs, we successfully cloned four healthy dogs through SCNT using the same donor genome from the skin of a male German shepherd old dog. Veterinary diagnosis (X-ray/3D-CT imaging) revealed that two cloned dogs showed normal phenotypes, whereas the others showed abnormal shortening of the mandible (brachygnathia inferior) at 1 month after birth, even though they were cloned under the same conditions except for the oocyte source. Therefore, we aimed to determine the genetic cause of brachygnathia inferior in these cloned dogs. To determine the genetic defects related to brachygnathia inferior, we performed karyotyping and whole-genome sequencing (WGS) for identifying small genetic alterations in the genome, such as single-nucleotide variations or frameshifts. There were no chromosomal numerical abnormalities in all cloned dogs. However, WGS analysis revealed variants of Wnt signaling pathway initiators (WNT5B, DVL2, DACT1, ARRB2, FZD 4/8) and cadherin (CDH11, CDH1like) in cloned dogs with brachygnathia inferior. In conclusion, this study proposes that brachygnathia inferior in cloned dogs may be associated with variants in initiators and/or regulators of the Wnt/cadherin signaling pathway.

​Labrador Retriever a Perfect Apartment Dog- A Review

Dogs are considered to be the most loyal companion of Mankind since ages. They are considered to be the decedents of wolfs. They hold their decent from antient extinct species of wolfs and recently available grey wolfs. Owing to its loyalty and affectionate nature they are the first domesticated animal in man’s world for 15000 years ago. Firstly, they were used by hunters during the time of hunting and were used as to gather the hunts, as in those days’ agriculture was not in practice. This long relationship between human and dogs made them quite loyal and adaptable to human needs. They can eat anything which a man can provide to them and happily accommodates with Human. There are large number of dogs breed which is available in various countries having their own individual traits. They vary in their shape, size, colours, behaviour and their sensory capabilities. Some of dogs are calm and some are aggressive in nature, due to which their utility changes, as they perform many roles for mankind, such as hunting, herding, pulling loads, protection, assisting police and the military, companionship, therapy and aiding disabled people. This relationship with mankind has earned them a title “Man’s best friend”. There are many popular dog breeds in India such as 1) Beagles 2) German Shepherd 3) Great Dane, 4) Labrador Retriever, 5) Boxer, 6) Rottweiller, 7) Pug, 8) Golden Retriever, 9) Cocker Spaniel 10) Dachshund etc.

INFLUENCE OF THE TYPE OF FEEDING ON THE REPRODUCTIVE ABILITIES OF SERVICE DOGS

The article presents the results of a study of indicators of reproductive ability of dogs of the German Shepherd breed when fed with natural products and dry food. The indices of reproductive function of dogs, multiple births, stillbirth and preservation of puppies for weaning were studied. Statistically significant differences were established in some indicators of the reproductive ability of the German Shepherd, which allows us to conclude about the possible influence of the type of feeding on these traits.

A Study into the Pawprints of Urban Dogs

The aim of the article is to present a comprehensive study on specific paw parameters and features, movement peculiarities, leg injuries, hairiness, etc. of dogs that lived in the city of Vilnius and its surroundings and left their marks on clay ware made by local craftsmen. The study was based on 238 clay objects found in Vilnius and dating from the late thirteenth to the eighteenth century. Dogs were found to leave the highest number of pawprints on bricks (90.8%), with significantly lower numbers on floor tiles (8%) and roof tiles (1.2%). The apparent increase in the number of traces is recorded from approximately the fifteenth century due to the steady increase in the quantity of clay products and the accessibility of the production environment to domestic animals. More than 450 dog pawprints in clay were examined using a trasology technique. Most frequently, one, two, or three traces were found on an artefact, while four, five, or six traces on one object were much less frequent. The analysis of trace features showed that 179 of the traces were those of the forepaws and 112 of the hind paws. The assessment of the deformation of the clay articles during firing and the comparison of the data obtained with the parameters of the paws of current breeds of dogs suggest that about 2% of the pawprints were left by small or young dogs. Mediumsized dogs left 70.5% of the traces, 25.7% belonged to dogs resembling current breeds of Doberman or Labrador retriever, 4.7% dogs similar to Borzoi or German shepherd, and 0.9% to Akita or Bobtail. An additional comparison of the pawprints with those of the present-day Lithuanian hounds showed that this type of dog could have left about 22% of the total number of the paw prints studied. It can be concluded that the results of the tracing studies revealed a significantly broader picture than the zooarchaeological material. Pawprint studies show that 25% of the dogs in the city of Vilnius during the period in question were long-haired and 75% were short-haired. The recorded paw pathologies provided information on various nail and toe injuries, tendon pathologies, and one possible case of patella dislocation. Meanwhile, the analysis of the dogs’ speed of movement suggests that at least some of the dogs in Vilnius between the late thirteenth and the eighteenth century were not tied and moved freely in the environment of the craftsmen who manufactured clay ware. In summary, the preliminary results of the pawprint analysis make a significant contribution to the broader knowledge of the history of dogs and provide unique data on urban dogs, which are scarcely recorded in written and zooarchaeological sources.

Surgical Treatment of Pediatric Dog-bite Wounds: A 5-year Retrospective Review

Introduction: Dog bites are a significant health concern in the pediatric population. Few studies published to date have stratified the injuries caused by dog bites based on surgical severity to elucidate the contributing risk factors. Methods: We used an electronic hospital database to identify all patients ≤17 years of age treated for dog bites from 2013–2018. Data related to patient demographics, injury type, intervention, dog breed, and payer source were collected. We extracted socioeconomic data from the American Community Survey. Data related to dog breed was obtained from public records on dog licenses. We calculated descriptive statistics as well as relative risk of dog bite by breed. Results: Of 1,252 injuries identified in 967 pediatric patients, 17.1% required consultation with a surgical specialist for repair. Bites affecting the head/neck region were most common (61.7%) and most likely to require operating room intervention (P = 0.002). The relative risk of a patient being bitten in a low-income area was 2.24, compared with 0.46 in a high-income area. Among cases where the breed of dog responsible for the bite was known, the dog breed most commonly associated with severe bites was the pit bull (relative risk vs German shepherd 8.53, relative risk vs unknown, 3.28). Conclusion: The majority of injuries did not require repair and were sufficiently handled by an emergency physician. Repair by a surgical specialist was required <20% of the time, usually for bites affecting the head/neck region. Disparities in the frequency and characteristics of dog bites across socioeconomic levels and dog breeds suggest that public education efforts may decrease the incidence of pediatric dog bites.

VEGF-B, VEGF-A, FLT-1, KDR, ERBB2, EGFR, GRB2, RAC1, CDH1 and HYAL-1 Genes Expression Analysis in Canine Mammary Gland Tumors and the Association with Tumor ClinicoPathological Parameters and Dog Breed Assessment

Canine mammary gland tumors (CMTs) are one of the most prevalent cancers in dogs and a good model for human breast cancer (BC), however gene expression analysis of CMTs is scarce. Although divergence of genes expression has been found in BC of different human races, no such research of different dog’s breeds has been done. The purpose of this study was to investigate expression of the VEGF-B, VEGF-A, FLT-1, KDR, ERBB2, EGFR, GRB2, RAC1, CDH1 and HYAL-1 genes of canine mammary carcinomas, compare the expression levels with clinicopathological parameters and analyze expression disparities between different breeds. Carcinomas and adjacent tissues were collected from female dogs to perform routine histopathology, immunohistochemistry (IHC) and quantitative real-time polymerase chain reaction (qRT-PCR). We found that VEGF-B and EGFR genes were overexpressed in the mammary gland carcinomas compared to adjacent tissue. VEGF-B gene expression had associations with different parameters (tumor size, grade, and absence of metastasis). Furthermore, differences in VEGF-B, FLT1, ERBB2, GRB2, RAC1, CDH1 and HYAL-1 genes expression have been found in different breed dogs (German Shepherd, Yorkshire Terrier) and mixed-breed dogs indicating that a dog’s breed could determine a molecular difference, outcome of cancer and should be accounted as a confounding factor in the future gene expression research.

The influence of Electroacupuncture on a dog diagnosed with osteoarthritis: A case report

Electroacupuncture is a specific branch of acupuncture that uses electrical stimulation through the selected acupoints. Osteoarthritis is considered a complex condition associated with painful joints and locomotor dysfunction. The aim of this case report was to bring scientific support regarding the effect of electroacupuncture in a dog with chronic joint degeneration. An 11-year-old male German shepherd referred to the University of Agricultural Sciences and Veterinary Medicine of Cluj with severe pain in his hind limbs and around his back. Diagnosis based on a western examination, neurological assessment and radiographs indicated chronic osteoarthritis with hip dysplasia. From Traditional Chinese Veterinary Medicine based diagnosis was Kidney Qi Deficiency leading to Bony Bi Syndrome. For the last two years, the treated dog with Mavacoxib (single dose every month) did not show any significant improvement. A combination of a fine needle with dry acupuncture, electroacupuncture (30-40Hz alternated with 80-100 Hz) and aqua-acupuncture using Zeel (AP) was performed. During the winter, weekly treatment was planned, after that, every two weeks treatment with electroacupuncture and dry needle, for five months until the present. Since we started the acupuncture treatment, the dog is more active and enjoys playing again. We have managed to stop the administration of NSAID’s and improve his life quality. In the present study, we evaluated the effect of complementary medicine on a dog with chronic pain and joint degeneration. Electroacupuncture is a complex technique that requires special training; if used wisely, it can be an excellent complementary therapy for veterinary patients’ pain control.

Autologous Bone Marrow Mononuclear Cells (BMMCs) for the Treatment of Uncomplicated Grade 2 Ununited Anconeal Process (UAP) in Six Dogs: Preliminary Results

The aim of this study was to report the results of autologous bone marrow mononuclear cell (BMMC) transplantation as a minimally invasive treatment for grade 2 UAP in dogs. This was an observational case series on six German shepherd dogs affected by grade 2 UAP as defined according to their clinical condition as well as radiographic and CT findings. Bone marrow was collected from the iliac crest and the mononuclear fraction was separated with density gradient centrifugation. Cells were suspended in fibrin glue before BMMC administration and implanted via transcutaneous injection under IB or CT guidance, using a spinal needle directly inserted into the ossification centre between the anconeal process and the olecranon. Clinical and radiographic follow-up was performed for up to 6 months. Microradiographic assessment was performed on one dog that died of other causes. A progressive reduction of pain within 3 weeks after BMMC administration was observed in all dogs, with gradually increased weight bearing on the affected limb. Radiographic and CT follow-up revealed the progressive fusion of the ossification centre at 90 days without any signs of secondary OA. The examination of microradiographs showed newly formed bone tissue in which a residue of calcified cartilage was present at the site of BMMC implantation. On the basis of these results, BMMC therapy for grade 2 UAP may be considered to be an effective and minimally invasive treatment option for dogs.