Characterization of erythromycin resistance in Campylobacter coli and Campylobacter jejuni isolated from pig offal in New Zealand

Campylobacter species are a group of spiral-shaped bacteria that can cause disease in humans and animals. We developed a high-affinity monoclonal antibody (MAb) probe that recognizes Campylobacter jejuni cells. Cell suspensions grown under microaerobic conditions at 42°C for 20 h on Bolton agar plates with lysed horse blood were used as live and heat-killed preparations, centrifuged at 8,000 × g for 20 min, and resuspended in carbonate buffer (pH 9.6) for coating on the enzyme-linked immunosorbent assay plates. BALB/c mice were immunized with C. jejuni sonicated cells at 107 CFU/ml to generate MAb-producing hybridoma clones. Of about 500 initial hybridoma clones, MAb 33D2, which reacted with C. jejuni and Campylobacter coli, was selected for further evaluation. MAb 33D2 is in the immunoglobulin subclass G2a and had relatively weaker reactivity with the C. coli strains tested. MAb 33D2 did not show any cross-reactions with the nine non-Campylobacter bacteria tested in the enzyme-linked immunosorbent assay and had a stronger affinity for C. jejuni as live versus heat-killed cells. In Western blot assays, MAb 33D2 recognized two major antigens of 62 and 43 kDa in extracts from C. jejuni cells but only one antigen of 62 kDa in extracts from C. coli cells.

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Molecular characterization of katA from Campylobacter jejuni and generation of a catalase-deficient mutant of Campylobacter coli by interspecific allelic exchange

Microbiology ◽

10.1099/13500872-141-6-1369 ◽

1995 ◽

Vol 141 (6) ◽

pp. 1369-1376 ◽

Cited By ~ 60

Author(s):

K. A. Grant ◽

S. F. Park

Keyword(s):

Molecular Characterization ◽

Campylobacter Jejuni ◽

Campylobacter Coli ◽

Deficient Mutant ◽

Allelic Exchange

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Cloning, nucleotide sequence and characterization of a gene encoding superoxide dismutase from Campylobacter jejuni and Campylobacter coli

Microbiology ◽

10.1099/13500872-140-5-1203 ◽

1994 ◽

Vol 140 (5) ◽

pp. 1203-1208 ◽

Cited By ~ 38

Author(s):

D. Purdy ◽

S. F. Park

Keyword(s):

Superoxide Dismutase ◽

Nucleotide Sequence ◽

Campylobacter Jejuni ◽

Campylobacter Coli ◽

Gene Encoding

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Characterization of campylobacter jejuni and campylobacter coli isolated from waste water

Zentralblatt für Bakteriologie Mikrobiologie und Hygiene Series A Medical Microbiology Infectious Diseases Virology Parasitology ◽

10.1016/s0176-6724(88)80095-6 ◽

1988 ◽

Vol 269 (2) ◽

pp. 188-196

Author(s):

Wolfgang Stelzer ◽

Hanspeter Mochmann ◽

Ursula Richter ◽

Hans-Joachim Dobberkau

Keyword(s):

Waste Water ◽

Campylobacter Jejuni ◽

Campylobacter Coli

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Comparative characterization of the virulence gene clusters (lipooligosaccharide [LOS] and capsular polysaccharide [CPS]) for Campylobacter coli, Campylobacter jejuni subsp. jejuni and related Campylobacter species

Infection Genetics and Evolution ◽

10.1016/j.meegid.2012.12.010 ◽

2013 ◽

Vol 14 ◽

pp. 200-213 ◽

Cited By ~ 24

Author(s):

Vincent P. Richards ◽

Tristan Lefébure ◽

Paulina D. Pavinski Bitar ◽

Michael J. Stanhope

Keyword(s):

Campylobacter Jejuni ◽

Capsular Polysaccharide ◽

Virulence Gene ◽

Gene Clusters ◽

Campylobacter Coli ◽

Comparative Characterization ◽

Campylobacter Species

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Inaccuracy of routine susceptibility tests for detection of erythromycin resistance of Campylobacter jejuni and Campylobacter coli

Clinical Microbiology and Infection ◽

10.1111/j.1469-0691.2009.02755.x ◽

2010 ◽

Vol 16 (1) ◽

pp. 51-56 ◽

Cited By ~ 13

Author(s):

M.T. van der Beek ◽

E.C.J. Claas ◽

D.J. Mevius ◽

W. van Pelt ◽

J.A. Wagenaar ◽

...

Keyword(s):

Campylobacter Jejuni ◽

Campylobacter Coli ◽

Erythromycin Resistance ◽

Susceptibility Tests

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Characterization of Campylobacter jejuni and Campylobacter coli strains isolated in the region of Nis, Serbia

Srpski arhiv za celokupno lekarstvo ◽

10.2298/sarh1012721m ◽

2010 ◽

Vol 138 (11-12) ◽

pp. 721-725 ◽

Cited By ~ 1

Author(s):

Biljana Miljkovic-Selimovic ◽

Lai-King Ng ◽

Lawrence Price ◽

Branislava Kocic ◽

Tatjana Babic

Keyword(s):

Campylobacter Jejuni ◽

Campylobacter Coli ◽

Infective Complication ◽

Strain Diversity ◽

Serotype O ◽

Heat Stable ◽

O 19 ◽

Chronic Sequelae ◽

Campylobacter Spp

Introduction. Campylobacter jejuni and Campylobacter coli represent one of the main causes of bacterial diarrhoea in humans. Although the disease is usually mild and self-limiting, severe chronic sequelae may occur, such as reactive arthritis, Guillain-Barr? and Miller Fisher syndromes. Serotyping is used as an epidemiological marker, while post-infective polyneuropathies are associated with several O serotypes. Objective. Strains of C. jejuni and C. coli were serotyped based on heat stable (HS) and heat labile (HL) antigens, as well as biotypes to determine strain diversity. Methods. Campylobacter spp. was isolated using selective blood media with antibiotics. Differentiation to the species level was done by a combination of biotyping tests and by a PCR-based RFLP test. The isolates were characterised by Penner and Lior serotyping methods. Results. The serotypes showed diversity without predominant serotypes. 24 HS serotypes were detected among 29 C. jejuni strains, and seven serotypes among nine C. coli strains. HL serotyping method successfully typed 62.5% of strains. Among 16 C. jejuni strains 14 serotypes were detected, and three among four C. coli strains. A C. jejuni strain associated with a patient with Guillain-Barr? syndrome was typed as biotype II, O:19. Conclusion. The biotyping and serotyping results have indicated that C. jejuni and C. coli strains in the region of Nis, Serbia are diverse and could be probably of unrelated sources of origin or reservoirs. The strain associated with the Guillain-Barr? syndrome patient was serotype O:19, one of the most common in this post-infective complication.

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